Journal of Bangladesh Society of Physiologist 2020-07-30T07:51:33+00:00 Dr Sultana Ferdousi Open Journal Systems <p>Established in 2006 by the Bangladesh Society of Physiologist Full text articles available.</p><p>Journal of Bangladesh Society of Physiologist is included on <a title="DOAJ" href="" target="_blank">DOAJ</a>.</p> Homocysteine, vitamin B<sub>12</sub> and folic acid levels in metabolic syndrome 2020-07-30T07:51:20+00:00 Fatema Nurjahan Khaleda Akter Shelina Begum <p><strong>Background: </strong>Metabolic syndrome (MeS) is a risk factor for cardiovascular disease, chronic lung disease, fatty liver disease and kidney disease<strong>. </strong>Elevated serum homocysteine level is an independent risk factor for cardiovascular disease and lung disease. Vitamin B<sub>12</sub> and folic acid deficiency causes hyperhomocysteinemia.</p> <p><strong>Objective: </strong>To measure serum homocysteine, vitamin B<sub>12</sub> and folic acid levels in metabolic syndrome.</p> <p><strong>Methods: </strong>This cross sectional study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbagh, Dhaka from March 2019 to February 2020. Total 30 female metabolic syndrome patients aged 25 to 45 years and 30 age and sex matched apparently healthy female subjects were enrolled for this study. The patients were selected from the outpatient department of Endocrinology of BSMMU. Fasting plasma glucose, lipid profile, serum creatinine, serum alanine aminotransferase levels were measured by autoanalyzer and serum homocysteine, vitamin B<sub>12</sub> and folic acid levels were measured by Chemiluminescent immunoassay. For statistical analysis, independent sample ‘t’test was done.</p> <p><strong>Results: </strong>In this study, the mean value of serum homocysteine level was significantly higher in MetS than those of control group (p value &lt;0.05). In addition, 16.67% MetS patients had hyperhomocysteinemia. <strong>Conclusion: </strong>From this study, it may be concluded that elevated homocysteine is associated with MetS.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 1-5</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Relation of body mass index (BMI) with cardiovascular response to head up tilt in healthy subjects 2020-07-30T07:51:22+00:00 Sadia Afrin Rimi Shamima Sultana Iffat Rezwana Sultana Ferdousi <p><strong>Background: </strong>Tilt table test is used for the last few decades to detect cause in unexplained syncope. The response to tilting may vary physiologically with obesity.</p> <p><strong>Objective: </strong>To assess the relationship of BMI to cardiovascular response to tilting.</p> <p><strong>Methods: </strong>This experimental study was conducted from March 2019 to Feb 2020 on 90 healthy subjects with different BMI. Fifty one subjects of both gender with BMI 18.5-24.9 kg/m<sup>2</sup> were included in the non-obese group and 39 subjects of both gender with BMI of 25-29.9 kg/m<sup>2</sup> were included in overweight group and they were further subdivided into male and female. Head up tilting was done for 10 minutes at 600 by using a motorized tilt table. Systolic blood pressure (SBP), diastolic blood pressure (DBP) were recorded by an automatic sphygmomanometer. Heart rate (HR) and peripheral capillary oxygen saturation (SpO<sub>2</sub>) were measured by a pulse oximeter. For statistical analysis, Independent sample ‘t’ test, Pearson’s correlation test and Chi square tests were applied.</p> <p><strong>Results: </strong>Significantly smaller rise of heart rate was observed in overweight males and greater fall of systolic blood pressure was observed in overweight females.</p> <p><strong>Conclusion: </strong>This study concluded that over weight is associated with reduced orthostatic tolerance to head up tilt test in both genders.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 6-10</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Cyclophosphamide induced anemia in Wistar rats: can it be prevented by Sajna (Moringaoleifera)? 2020-07-30T07:51:23+00:00 Farhana Fatema Sharmin Nahar Qazi Shamima Akhter <p><strong>Background: </strong>Chemotherapy-induced anemia is a multifactorial challenge to the treatment of malignancy. Herbal medicine may help totreat such anemia due to its natural compatibility<em>. Moringaoleifera </em>(Sajna), is well known for preventing and curing anemia.</p> <p><strong>Objective: </strong>To evaluate the preventive effect of aqueous extract of <em>Moringaoleifera</em>leaf (AEMOL) on cyclophosphamide (CP) induced anemia in Wistar rats.</p> <p><strong>Method</strong>: The experimental study was carried out in the Department of Physiology, Dhaka Medical College, Dhaka from July 2015 to June 2016. Thirty Wistar ratsof both sexes weighing 175±25 gm were grouped as A[normal saline (1ml/kg); oral],and B [CP (3mg/kg);i. p.], C[AEMOL (300mg/kg);oral], D [AEMOL(300mg/kg); oral and CP(3 mg / kg) i. p. simultaneously for 14 consecutive days] and E [AEMOL(300mg/kg); oralfor 14 consecutive days and CP(30mg/ kg); i. p. on 12<sup>th</sup>, 13<sup>th</sup>,14<sup>th</sup> day] with 6 rats in each group.All the rats had free access to food and distilled water during the period of acclimatization (seven days) and experiment(fourteen days).Total count(TC)) of RBC, hemoglobin (Hb) level and packed cell volume (PCV)were estimated to assess anemia in all rats before and after experiment. The statistical analyses were done by one way ANOVA followed by Bonferroni post hoc test where p&lt;0.05 was accepted as level of significance.</p> <p><strong>Result: </strong>The mean TCof RBC, Hb% and PCV were significantly (p&lt;0.001) lower in CP treated rats (group B), in comparison to all other rats of different groups (A, C, D and E). In addition, all these three variables were significantly higher in AEMOLtreated group(C) compared to saline treated rats (A)but almost similar in rats with two groups of different dose schedule of AEMOL and CP (D, E).</p> <p><strong>Conclusion:</strong>From the study, it can be concluded that CP induced anemia can be prevented by AEMOL used either simultaneously or in an anticipating manner.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 11-16</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Alteration of serum calcium and magnesium level in transfusion dependent thalassemic patients with combined iron chelator therapy 2020-07-30T07:51:25+00:00 Fatema Tuz Munira Shelina Begum SFH Urmi <p><strong>Background: </strong>Regular blood transfusion and iron chelation is the primary treatment of thalassemia patientsto maintain their life. Iron chelatormay alter serum total calcium and magnesium level in TDT patients.</p> <p><strong>Objective: </strong>To evaluateany alteration of serum total calcium and magnesium level in transfusion dependent thalassemia patients treated with iron chelator.</p> <p><strong>Method: </strong>The present cross sectional study was carried out in the department of Physiology, BSMMU, Dhaka between September 2017 to February 2019. Thirty cases of TDT, aged 5-40 year were included in the study group. Age and sex matched 30 healthy subjects were also studied as control. All the TDT patients were selected from the outpatient Department of Hematology and Transfusion Medicine, BSMMU, Dhaka. Serum total calcium, magnesium and ferritin levels were measured by colorimetric method. For statistical analysis independent sample t test and Chi-Square test and Pearson correlation coefficient test were used.</p> <p><strong>Result: </strong>The mean serum total calcium and magnesium levels were significantly (p&lt;0.05) lower and serum ferritin level was significantly (p&lt;0.001) higher in study group compared to that of control. Again, in this study group 6.7 % TDT patients had hypocalcemia and 13.3% TDT patients had hypomagnecemia.</p> <p><strong>Conclusion: </strong>This study may conclude that low serum total calcium and magnesium level may be associated with TDT patients treated by combined deferoxamine (DFO) &amp; deferiprone (DFP) iron chelator.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 17-22</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Vitamin D<sub>3</sub> supplementation on plasma antioxidant enzymes in D<sub>3</sub> deficient patients with COPD - a randomized controlled trial 2020-07-30T07:51:26+00:00 Salma Anjum Taskina Ali Kazi Saifuddin Bennoor Md Ali Hossain Md Saiful Islam Maksuda Bintey Mahmud Samia Hassan Salsa Bil Nahar Sharkia Khanam Rosy Naznin Sultana Khalada Akter <p><strong>Background: </strong>Free radical is a crucial factor for progression of COPD. Antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT) have been well known to reduce morbidity of chronic disease. Vitamin D<sub>3</sub> has antioxidant effect in human body.</p> <p><strong>Objectives: </strong>To assess the effects of D3 supplementation on plasma SOD and CAT levels in D<sub>3</sub> deficient COPD patients.</p> <p><strong>Method: </strong>A double blinded placebo controlled randomized clinical trial was carried out on 30 vitamin D<sub>3</sub> deficient male, smoker and stable COPD patients of age &gt;40 years. All the patients were randomly allocated to ‘Study’ (n=15) or ‘Control’ (n=15) and their baseline plasma SOD and CAT were measured. Study patients received 80,000 IU (2 oral capsules) of D<sub>3</sub> per week for first 13 weeks. Subsequently, after checking their serum 25(OH)D or Ca2+, they received 40,000 IU (1 oral capsule) of D<sub>3</sub> either per 1 week or per 2 weeks or per 6 weeks or no further supplementation for next 13 weeks. All the ‘Control’ patients received two oral capsules of placebo weekly for consecutive 26 weeks. Additionally, all patients of both groups were also advised to have sunlight exposure (within 11 to 14 hrs) at least for 5 to 15 minutes daily. After 26 weeks of follow up, both enzymes, serum 25(OH)D or Ca<sup>2+</sup>of all patients were measured by spectrophotometry. Data were analyzed by paired sample and independent sample ‘t’ test.</p> <p><strong>Results: </strong>The baseline plasma SOD (14.80±3.86U/ml vs 11.95±4.03U/ml) and CAT (11.0±7.59U/ml vs14.41±4.83U/ml) in study and control was almost similar (p&gt;0.05). However, the mean SOD (18.98±2.98U/ml) and CAT (32.75±9.70U/ml) significantly increased (p&lt;0.01; p&lt;0.001) after 26 weeks of follow up only in ‘Study’ patients. In addition, both enzymes were significantly (p&lt;0.001) higher in the D<sub>3</sub> supplemented patients than those of placebo treated patients after 26 weeks of follow up.</p> <p><strong>Conclusion: </strong>The present study reveals that vitamin D<sub>3</sub> supplementation increases the plasma antioxidant enzymes in D<sub>3</sub> deficient patients with COPD.</p> <p><strong>Trial registration: </strong> identifier NCT04011930</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 23-32</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Role of blood cells in diagnosis of asthma-COPD overlap among patients with COPD 2020-07-30T07:51:28+00:00 Md Monimul Islam Md Ali Hossain Kazi Saifuddin Bennoor Taskina Ali <p><strong>Background: </strong>As asthma-COPD overlap (ACO) and COPD share some common clinical features, this makes difficult to distinguish them from each other. Since, both of them are characterized by airway inflammation, any inflammatory biomarker such as total count (TC) of white blood cells (WBC), neutrophil, eosinophil and platelet can be used for that purpose.</p> <p><strong>Objective: </strong>To assess the role of TC of WBC, neutrophil, eosinophil and platelet in diagnosis of ACO among patients with COPD.</p> <p><strong>Methods: </strong>This cross sectional study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University, Dhaka from 2019 March to Feb 2020. In this study, 63 male stable patients (age range: 40 to 80 years) of COPD were enrolled. Among them, 51 patients were finally selected according to exclusion criteria and divided into two groups, ACO (n=26) and COPD (n=25). TC of WBC, neutrophil, eosinophil and platelet of all patients were measured by standard lab procedure. For statistical analysis, Chi-Square and Fisher exact test, Shapiro- Wilk test, Mann-Whitney U test were done as applicable. More over, receiver operating characteristic (ROC) curve (AUC) and optimal cutoff values, ROC curve analysis was performed.</p> <p><strong>Results: </strong>In the present study, TC of WBC and neutrophil count were significantly (p&lt;0.01) lower, whereas, blood eosinophil count was significantly (p&lt;0.05) higher in ACO patients than those of COPD. In addition, highest area under the receiver operating characteristic curve was found in case of blood neutrophil count, followed by TC of WBC and eosinophil count. Moreover, at optimal cut off value, the sensitivity, specificity, positive predictive value, negative predictive value of blood count of total WBC were calculated as, 72.00, 61.50, 64.28, 69.56, respectively. However, the aforementioned indices were observed for blood neutrophil count, as 72.00, 76.90, 75.00, 74.07 and for blood eosinophil count, as 38.50%, 92.00%, 83.33%, 58.97%, respectively.</p> <p><strong>Conclusion:</strong>The present study reveals that blood count of total WBC, neutrophil and eosinophil, but not the platelet, play substantial role in diagnosis of ACO among the patients with COPD.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 33-38</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Effect of music therapy on heart rate variability in generalized anxiety disorder: A poincaré analysis 2020-07-30T07:51:30+00:00 Bithi Mallik Sultana Ferdousi Shamima Sultana <p><strong>Background: </strong>Poincare plot analysis of heart rate variability (HRV) is a complex nonlinear method for assessing cardiac autonomic nerve function (CANF) in generalized anxiety disorder (GAD).</p> <p><strong>Objective: </strong>This study aimed to observe the effect of music therapy on HRV by poincaré plot analysis in GAD patients.</p> <p><strong>Methods: </strong>This prospective Interventional study was done in 2019 on 60 newly diagnosed GAD patients aged 20- 40 years, both male and female. Among them, 30 patients were under music therapy with Raga Bhairabi based Rabindra Sangeet for 3 months and 30 patients were without music therapy and both patient groups were studied over three months. Healthy controls, who were enrolled in this study, were Age, sex and BMI matched. Poincaré plot (non linear measure) of HRV of all GAD patients and control were recorded at baseline and after three months of follow up. HRV were recorded by Power Lab 8/ 35. For statistical analysis Bonferroni’s Post Hoc test and paired sample ‘t’ test were done.</p> <p><strong>Results:</strong>Standard deviation of the points perpendicular to the axis of line of identity(SD1), standard deviation of the points along the axis of line of identity (SD2) SD and SD1/SD2 were significantly lower in all GAD patients compared to healthy control at baseline. After 3 months of music therapy, significant increment of these parameters occurred in GAD patients, whereas no changes of these except SD1 were noted in patients without music therapy after 3 months. Moreover, these parameters were not significantly different in patients under music therapy compared to control at the end of three months.</p> <p><strong>Conclusion</strong>: This study concluded that music therapy can improve cardiac autonomic dysfunction in GAD patients.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 39-45</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement## Relationship of heart rate variability with iron status in metabolic syndrome 2020-07-30T07:51:31+00:00 Halima Akter Munni Shelina Begum <p><strong>Background: </strong>Clustering of some most dreadful cardiovascular risk factors gives rise to metabolic syndrome (MetS). Higher iron status and impaired cardiac autonomic status may important play role in increased risk of cardiovascular morbidity in this group of patients.</p> <p><strong>Objective: </strong>To observe the relationship of HRV with iron status in patients with MetS.</p> <p><strong>Methods: </strong>This cross-sectional study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka from March, 2019 to February, 2020. For this study, 35 MetS female patients aged 25 to 45 years were enrolled in MetS group and equal number of age and sex matched apparently healthy subjects constituted control group. For evaluation of iron status, serum iron, serum ferritin, total iron binding capacity (TIBC) and transferrin saturation (Tsat) were measured by autoanalyzer. HRV was assessed by Powerlab 8/35, AD instruments, Australia. Data were expressed as mean±SD. Statistical analysis was done by Independent sample ‘t’ test and Pearson’s correlation coefficient test as applicable.</p> <p><strong>Results: </strong>In this study, resting pulse rate, systolic blood pressure (SBP), diastolic blood pressure (DBP) were significantly (p&lt;0.001) higher and mean heart rate, standard deviation of the RR intervals (SDRR),mean R-R interval, standard deviation of the difference betweensuccessive RR intervals (SDSD), square root of mean squared differences of successive RR intervals (RMSSD), proportion of difference of successive RR interval greater than 50 ms (pRR50) were significantly (p&lt;0.001) lower in MetS patients compared to control. Among the parameters of iron status, serum ferritin was significantly (p£&lt;0.05) higher and TIBCwas significantly(p&lt;0.05) lower in MetS patients in comparison to control. On correlation analysis, only the TIBC showed significant positive correlation with mean RR interval, SDRR, CVRR, SDSD, RMSSD, pRR50 (p&lt;0.05) in MetS patients.</p> <p><strong>Conclusion: </strong>This study reveals that poor parasympathetic activity is related to higher iron status in metabolic syndrome patients.</p> <p>J Bangladesh Soc Physiol. 2020, June; 15(1): 46-53</p> 2020-07-07T00:00:00+00:00 ##submission.copyrightStatement##