TY - JOUR AU - Biswas, Animesh AU - Bari, MA AU - Roy, Mohashweta AU - Bhadra, SK PY - 2009/03/17 Y2 - 2024/03/28 TI - In vitro Regeneration of Aristolochia tagala Champ. A Rare Medicinal Plant of Chittagong Hill Tracts JF - Journal of Bio-Science JA - J bio-sci. VL - 15 IS - 0 SE - Articles DO - 10.3329/jbs.v15i0.2204 UR - https://www.banglajol.info/index.php/JBS/article/view/2204 SP - 63-67 AB - <p>An efficient regeneration protocol through <em>in vitro </em>direct organogenesis was developed for a valuable medicinal plant <em>Aristolochia tagala </em>Champ. using nodal segments as explants. Multiple shoot buds were induced directly from nodal explants cultured on MS (Murashige and Skoog 1962) basal medium supplemented with 2.0 mg/l BAP (N6- benzylaminopurine) and 0.5 mg/l NAA (a-naphthalenacetic acid). The average number of shoots induced per culture was found to be six. Excised shoot roots were cultured on half-strength MS medium containing 0.5 mg/l IBA. The rooted plantlets were transferred to natural environment after proper acclimatization.</p> <p> <strong>Key words: </strong><em>Aristolochia tagala</em>, rare medicinal plant, direct organogenesis.</p> <p>doi: 10.3329/jbs.v15i0.2204</p> <p>J. bio-sci. 15: 63-67, 2007   </p> <br /><p> </p><p> </p> ER -