Integrated effects of manures and fertilizers on the yield and nutrient uptake by BRRI dhan 49

A field experiment was conducted at the Soil Science Field Laboratory of Bangladesh Agricultural University (BAU), Mymensingh during Aman season of 2011 to evaluate the effects of manures and fertilizers for maximizing the yield of BRRI dhan49. The experiment had six treatments, laid out in a randomized complete block design (RCBD) with four replications. The treatments were T0 = Control, T1 = STB-CF (HYG), T2 = CD + STB-CF (HYG), T3 = PM + STB-CF (HYG), T4= CoM + STB-CF (HYG) and T5 = Farmers’ practice (FP). Organic manures including cowdung, poultry manure and compost were applied to the experimental plots @ 5, 3 and 5 t ha -1 , respectively. The recommended dozes of N, P, K and S supplied from urea, TSP, MoP and gypsum were 90, 15, 60 and 15 kg ha -1 , respectively. Yield contributing characters like plant height, effective tillers hill -1 , panicle length and grains panicle -1 of BRRI dhan49 were significantly influenced by the application of manures and fertilizers. The highest grain yield of 4.87 t ha -1 was observed in the treatment T3 [PM + STB-CF (HYG)] and the lowest value of 3.61 t ha -1 was found in T0 (control). The straw yield ranged from 4.10 to 5.51 t ha -1 in different treatments. The NPKS uptake by BRRI dhan49 was markedly influenced by manures and fertilizers. Based on overall results, the treatment T3 [PM + STB-CF (HYG)] was found to be the best combination of manures and fertilizers for obtaining the maximum yield and quality of rice.

-1 , respectively.The recommended dozes of N, P, K and S supplied from urea, TSP, MoP and gypsum were 90, 15, 60 and 15 kg ha -1 , respectively.Yield contributing characters like plant height, effective tillers hill -1 , panicle length and grains panicle -1 of BRRI dhan49 were significantly influenced by the application of manures and fertilizers.The highest grain yield of 4.87 t ha -1 was observed in the treatment T3 [PM + STB-CF (HYG)] and the lowest value of 3.61 t ha Introduction Rice (Oryza sativa) is the staple food crop in Bangladesh and the cropping pattern of the country is predominately rice-based.In Bangladesh, rice dominates over all other crops and covers 77 % of the total cropped area and 93% farmers grow rice (BBS, 2011).The total area and production of rice in Bangladesh are about 11.7 million hectares and 31.98 million metric tons, respectively (BBS, 2011).
The soil fertility status in Bangladesh is gradually declining.The stagnating trend in the yield of major crops of the country has become an alarming issue for the scientist and policy makers (Bhuiyan, 1994).Low organic matter content of the soil, imbalanced use of chemical fertilizers, less use of organic manures and inadequate attention given for its improvement and maintenances are the major causes behind declining soil fertility in Bangladesh (Karim et al. 1994).
Soil organic matter (SOM) plays an important role in maintaining soil fertility and productivity.Organic matter acts as a reservoir of plant nutrients especially N, P and S and micro-nutrients and check leaching of the nutrients.The problem of nutrient deficiencies as well as nutrient mining caused by intensive cropping with HYV of rice and nutrient imbalance can be minimized by judicious application chemical fertilizers in combination with organic manures.Losses of SOM can only be replenished in the short term by application of organic matter such as manures.
Cowdung and poultry manure are the most popular and promising bulky organic manures produced from solid and liquid excreta of farm animals.They contain considerable amounts of essential nutrient elements required for plant growth.Hence, addition of a good amount of cowdung and poultry manure to the crop field is essential for soil fertility and productivity and maintenances of the SOM.Many farmer's use more amount of N fertilizer than needed while they use less amount of other fertilizers such as P, K and S.They seldom use micronutrient fertilizers e.g.Zn and B. This practice in turn produces a negative impact on crop production (Rijpma and Jahiruddin, 2004).Continuous use of inorganic fertilizer deteriorates soil properties and causes a nutrient imbalance of soil including micronutrient deficiency.Furthermore, chemical fertilizers pollute soil and water making environment even more harmful for both terrestrial as well as aquatic life.Application of inorganic fertilizer has always been expensive inputs for crop production, especially for developing country like Bangladesh.In near future, chemical fertilizer is likely to be even more costly.This situation in turn will pose a serious threat to food security of vast millions of people of Bangladesh.
The problem of nutrient deficiencies as well as nutrient mining caused by intensive cropping with high yielding varieties of rice and nutrient imbalance can be minimized by judicious application of nutrients through organic manures or chemical fertilizers.The present research work was, therefore, undertaken to find out effect of manures and fertilizers on the yield and nutrient uptake by BRRI dhan49.

Materials and Methods
The experiment was carried out in the Soil Science Field Laboratory of Bangladesh Agricultural University, Mymensingh during the Aman season of 2011.The soil belongs to Sonatala series under the Agroecological Zone (AEZ) of Old Brahmaputra Floodplain.The experimental soil was silt loam in texture having pH 6.18, organic matter content 2.15%, total N 0.124%, available P 6.51 ppm, exchangeable K 0.074 me/100 g soil, available S 14.85 ppm and CEC 12.5 me/100 g soil.BRRI dhan49, a high yielding variety of rice was used in this experiment as test crop.The experiment was laid out in a randomized complete block design (RCBD) with three replications.The experiment contained six treatments: T 0 = Control, T 1 = STB-CF (HYG), T 2 = CD + STB-CF (HYG), T 3 = PM + STB-CF (HYG), T 4 = COM + STB-CF (HYG) and T 5 = Farmers' practice (FP).Here, STB=Soil Test Basis, CF=Chemical fertilizer, CD = Cowdung, PM = Poultry manure, COM=Compost, FP=Farmers' practice, HYG=High yield goal.
Organic manures including cowdung, poultry manure and compost were applied to the experimental plots @ 5, 3 and 5 t ha -1 , respectively.The nutrient content of manures has been depicted in Table 1.The recommended dozes of N, P, K and S supplied from urea, TSP, MoP and gypsum were 90, 15, 60 and 15 kg ha -1 , respectively.Triple superphosphate (TSP), muriate of potash (MoP), and gypsum were applied as basal dose to all the experimental plots.The amounts of N, P, K, and S coming from cowdung, poultry manure and compost were deducted from recommended N, P, K and S fertilizer doses on IPNS basis.Cowdung, poultry manure and compost were incorporated in the plots as per treatments 10 days before transplanting of the rice seedlings.The manure was mixed thoroughly with the soil.Urea was applied in three equal splits.The first dose of urea was applied 15 days after transplanting of rice seedlings.The rest doses of urea were top dressed 30 (active tillering stage) and 45 days after transplanting (panicle initiation stage).Thirty-day old seedlings of BRRI dhan49 was transplanted in the plots maintaining a plant spacing of 20cm x 20cm.Three healthy seedlings were transplanted in each hill.Intercultural operations such as irrigation and weeding were done as and when necessary.The crop was harvested at full maturity.Grain yield was recorded on 14% moisture basis and straw yield on sun dry basis.Five hills were randomly selected from each plot at maturity to record the yield contributing characters.Grain and straw samples were analysed for N, P, K and S concentration following standard methods (Page et al., 1982).The NPKS uptake by grain and straw was calculated from the nutrient contents and yield data.All the data were statistically analyzed by F-test and the mean differences were adjudged by DMRT at 5% level (Gomez and Gomez, 1984).

Yield contributing characters
Yield contributing characters such as plant height, effective tillers hill -1 , panicle length and grains panicle -1 were influenced significantly due to application of organic manures and chemical fertilizers (Table 2).All the treatments significantly increased the plant height over control and the highest value (94.25 cm) was recorded due to the application of poultry manure @ 3 t ha -1 in combination with chemical fertilizers (T 3 ) which was statistically similar with T 2 [CD + STB-CF (HYG)].The lowest plant height (78.88 cm) was obtained in control (T 0 ).The maximum number of effective tillers hill -1 (13.50) was obtained in the treatment T 3 [PM + STB-CF (HYG)] and the minimum value of 9.00 was found in control.The longest panicle length of 25.55 cm was recorded in T 3 which was statistically similar with T 2 and T 4 .The shortest panicle length of 19.33cm was recorded in the control (T 0 ).The treatments T 1 [STB-CF (HYG)] and T 5 (FP) also increased the panicle length over the control.The number of grains panicle -1 ranged from 112.2 to 133.7.The treatment T 3 produced the highest number of grains panicle -1 which was statistically similar with T 2 .The lowest value was obtained in the treatment T 0 (112.2).The 1000-grain weight was not influenced significantly due to application of organic manures and chemical fertilizers.The 1000-grain weight ranged from 19.27 to 23.35 g.  Results in Table 3 show that the grain yield of BRRI dhan49 was significantly influenced due to different treatments.The grain yield ranged from 3.61 to 4.87 t ha -1 . The lowest grain yield was obtained in the control (T 0 ).The highest grain yield was found in T 3 [PM + STB-CF (HYG)] which was statistically similar with T 1 [STB-CF (HYG)] and T 4 [COM + STB-CF (HYG)].The grain yield due to different treatments may be ranked in the order of T 3 > T 1 > T 2 > T 4 > T 5 > T 0 .The percent increase in grain yield of BRRI Dhan49 over control due to different treatments ranged from 18.28 to 34.90.The treatment T 3 and T 5 gave the highest (34.90%) and the lowest (18.28%) yield increase over control, respectively.It reveals that BRRI dhan49 responded better to the nutrients supplied from organic manures rather than to chemical fertilizers.This might be due to the quick release of nutrients from poultry manure.

Straw yield
Straw yield of BRRI dhan49 was also influenced significantly by different treatments under study (Table 3).The straw yield ranged from 4.10 to 5.51 t ha -1 .It was observed that the treatment T 3 [PM + STB-CF (HYG)] produced the highest straw yield.The lowest straw yield was obtained in T 0 (control).The straw yields due to different treatments may be ranked in the order of T 3 > T 2 > T 4 > T 1 > T 5 >T 0 .The treatments under study resulted in 22.43% to 34.39% increase in straw yield over control.It appears that the treatment T 3 gave the highest straw yield increase of 34.39% over control.Table 2 also indicates that organic manures served as the better source of nutrients in producing straw yields of rice.Nitrogen uptake: The N uptake by grain and straw as well as total N uptake by BRRI dhan49 was significantly influenced by different treatments (Table 4).The N uptake by grain and straw ranged from 41.83 to 63.01 kg ha  , respectively (Table 4).The highest P uptake by grain was recorded in T 3 and the lowest P uptake by grain was found in the control.In case of straw, the highest P uptake was recorded in T 3 which was statistically similar T 2 .The lowest value was recorded the control (T 0 ).The treatments T 1 and T 5 were statistically similar in their effects on P uptake by straw.
Potassium uptake: Application of manures and chemical fertilizers significantly affect K uptake as well as total P uptake by BRRI dhan49 (Table 4).The K uptake by grain varied from 7.20 to 11.80 kg ha -1 while the values for straw ranged from 44.98 to 70.36 kg ha -1 . The highest K uptake of 11.80 kg ha -1 by grain was recorded in T 3 treatment which was statistically similar with the treatments T 2 treatments.In case of straw, the highest K uptake of 70.36 kg ha -1 was also recorded in T 3 treatment which was statistically similar with the treatments T 2 but significantly superior to all other treatments.Sulphur uptake: Sulphur uptake by grain and straw as well as total S uptake by BRRI dhan49 was significantly increased due to the application of organic manures and chemical fertilizers (Table 4).Sulphur uptake by grain and straw ranged from 4.28 to 6.71 kg ha -1 and 3.80 to 6.52 kg ha -1 , respectively.In case of grain, the highest S uptake was recorded in T 3 .The lowest uptake was recorded in the control.The treatment T 3 was statistically identical with T 2 but significantly different from all other treatments.In case of straw, the highest S uptake by BRRI dhan49 was recorded in T 3 .The lowest uptake was recorded in control.

Discussion
In the present study the effects of manures and fertilizers on the growth parameters and yield as well as nutrient uptake by BRRI dhan49 have been elaborated.From the results it is observed that the yield contributing characters such as plant height, number of effective tillers hill , and 1000-grain weight are higher in T 3 treatment where poultry manure was applied in combination with fertilizers on IPNS basis as compared to those observed in other treatments.Organic manures were found better source of nutrients in respect of their effects on yield attributes of rice.Among the manures, poultry manure showed superior performance on plant height.This might be due to slow release of nutrients from poultry manures and efficient utilization of nutrients by plants.These results are in agreement with Parvez et al. (2008) who observed that the plant height of rice was significantly influenced by the application of organic manures and fertilizers.The effect of poultry manure and cowdung was more pronounced in producing the number of effective tillers hill -1 as compared to chemical fertilizers.These results are well corroborated with the findings of Rajni Rani et al. (2001) who found increased number of effective tillers hill -1 with the integrated use of vermicompost, PM and nitrogenous fertilizers in rice.The results reveal that cowdung, compost and poultry manure and compost influenced markedly the panicle length.These results are in agreement with Singh et al. (2005) who found increased panicle length with the application of urea, cowdung, and Azospirillum, individually or in combinations.A significant increase in panicle length due to of organic manures and fertilizers N, S, Zn and B was also noted by Hoque (1999).Mondal et al. (1990) observed that the number of spikelet's panicle -1 of rice was increased with the increasing NPK rates and FYM application.Similar results were also reported by Chander and Pandey (1996).Razzaque (1996) noted a significant increase in grains panicle -1 due to application of organic manures and fertilizer nitrogen.The effect of poultry manure was more pronounced in producing filled grains panicle -1 . Umanah et al. (2003) reported that poultry manure increased the grains per panicle.Hoque (1999) noted significant increase in filled grains panicle -1 with the application of organic manures and fertilizers.These results are well corroborated with the findings of Rahman et al. (2009) who found an insignificant response of urea-N and manures on 1000-grain weight of BRRI dhan29.
Poultry manure was found more effective in producing grain yields of BRRI dhan49 as compared to cowdung and chemical fertilizers.These results are in agreement with the findings of Rajni Rani et al. (2001), Rahman et al. (2009) and Parvez et al. (2008).Poultry manure also demonstrated superior effect in producing straw yield of rice as compared to cowdung and chemical fertilizers.Ahmed and Rahman (1991) reported that the application of organic manure and chemical fertilizers increased straw yield of rice.
It is clear that the application of organic manures had positive influences on the N uptake by BRRI dhan49.Significant increase in N uptake by rice grain and straw with the application of organic manures and fertilizers was reported by Hoque (1999).Dongarwar et al. (2003) observed that the P uptake by rice grain was increased with the combined application of manures and fertilizers.Rahman et al. (2009) also found similar results with a trial on BRRI dhan29 using urea and manures.All the treatments receiving poultry manure and cowdung significantly increased the total K uptake by rice.It was observed that the K uptake by grain was much less than that by straw.These results are well corroborated with Meena et al. (2003) who reported that application of organic manure and chemical fertilizers significantly increased the K uptake by rice.The S uptake by rice was also influenced significantly due to application of manures and fertilizers.These results are in agreement with Akter et al. (2012) and Malika (2011) who found positive effects on S uptake by rice with application of manures and fertilizers.

Conclusion
From the present study it is observed that the application of manures and fertilizers showed better performance in respect of grain yield and yield contributing characters, nutrient uptake as compared to the application of fertilizers only.The performance of poultry manure was better than that of cowdung and compost for the growth and yield of rice.Application of poultry manure @ 3 t ha -1 in association with chemical fertilizers will be rewarding for the maximization of rice yield.

Figure
Figure(s) in a column having common letter (s) do not differ significantly at 5% level of significance.Here, STB = Soil Test Basis, CF = Chemical fertilizer, CD = Cowdung, PM = Poultry manure, CoM = Compost, FP = Farmers' practice, HYG = High yield goal, SE = Standard error of means; CV = Coefficient of variation The maximum N uptake by grain was recorded in T 3 [PM + STB-CF (HYG)].The treatment T 3 was statistically similar with T 1 [STB-CF (HYG)], T 2 [CD + STB-CF (HYG)], and T 4 [COM+ STB-CF (HYG)] but significantly different from other treatments.The lowest grain N uptake of 41.83kg ha -1was recorded in the control.The highest N uptake by straw was recorded in T 3 which was statistically similar with the treatments T 2 and T 4 but significantly different from all other treatments.The minimum N uptake by straw of 23.53 kg ha -1 was recorded in the control.

Table 1
Nutrient contents in cowdung, compost and poultry manure

Table 2 .
Effect of manures and fertilizers on the yield components of BRRI dhan49

Table 3 .
Grain and straw yields of BRRI dhan49 as influenced by manures and fertilizers

Table 4 .
The uptake of NPKS by BRRI dhan49 as influenced by integrated use of manures and fertilizers