TY - JOUR AU - Thongkao, Kanittada AU - Sudjaroen, Yuttana PY - 2020/03/12 Y2 - 2024/03/28 TI - Screening of antibiotic resistance genes in pathogenic bacteria isolated from tiny freshwater shrimp (Macrobrachium lanchesteri) and “Kung Ten”, the uncooked Thai food JF - Journal of Advanced Veterinary and Animal Research JA - J. Adv. Vet. Anim. Res VL - 7 IS - 1 SE - Original Articles DO - UR - https://www.banglajol.info/index.php/JAVAR/article/view/46368 SP - 83-91 AB - <p><strong>Objective: </strong>This study aimed to isolate and identify of pathogenic bacteria in tiny freshwater shrimp (<em>Macrobrachium lanchesteri</em>) and in Kung Ten, which is an unusual Thai cuisine that eaten alive shrimp directly. Antimicrobial susceptibility test and identification of antibiotic resistance genes for isolated bacteria were conducted.</p><p><strong>Materials and Methods: </strong>Eighty of fresh shrimp samples and forty of Kung Ten salads were collected from four fresh markets, which were located in Bangkok and Nonthaburi province (<em>N </em>= 120). The isolation, identification, and antimicrobial susceptibility test of pathogenic bacteria were done following the Clinical and Laboratory Standards Institute guidelines. Antibiotic-resistant bacteria were screened for β-lactamase relating genes, such as <em>Amp</em>C (<em>MOX </em>and <em>ACC </em>genes), <em>bla</em>CTX-M, and <em>Int1 </em>genes.</p><p><strong>Results: </strong>The number of bacterial isolates in tiny freshwater shrimp and Kung Ten salad was 136 and 65, respectively. <em>Aeromonas caviae</em>, <em>A. hydrophilla</em>, <em>Proteus penneri</em>, <em>Proteus vulgaris</em>, and <em>Klebsiella pneumoniae </em>were commonly found. Ampicillin, amoxicillin/clavulanic, cefuroxime, tet­racycline, and trimethoprim/sulfamethoxazole resistance were observed, and common antibiot­ic-resistant bacteria were <em>A. caviae</em>, <em>P. vulgaris</em>, <em>Enterobacter Aerogenes</em>, and <em>K. pneumoniae</em>. <em>A. caviae</em>, <em>P. penneri</em>, <em>K. Pneumoniae</em>, and <em>A. hydrophilla </em>were positive for <em>MOX </em>gene; <em>bla</em>CTX-M, and <em>Int1 </em>genes; <em>ACC </em>and <em>Int1 </em>genes; and <em>ACC </em>gene, respectively.</p><p><strong>Conclusion: </strong>Raw or uncooked shrimps in Kung Ten salad may a risk in foodborne diseases due to positive for pathogenic bacterial isolates. However, hygienic control on food preparation is diffi­cult to apply because of the difficulty of changing in local Thai food behavior.</p><p>J. Adv. Vet. Anim. Res., 7(1): 83-91, March 2020</p> ER -