@article{Wahyuni_Sunarso_Prasetiyono_Satrija_2019, title={Exploration of anthelmintic activity of Cassia spp. extracts on gastrointestinal nematodes of sheep}, volume={6}, url={https://www.banglajol.info/index.php/JAVAR/article/view/41766}, abstractNote={<p><strong>Objective: </strong>This study aimed to explore the phytochemical constituents and anthelmintic activities of four <em>Cassia </em>spp. leaves against <em>Haemonchus contortus</em>.</p> <p><strong>Materials and Methods: </strong>The extracts were prepared from four species of <em>Cassia </em>spp. (<em>C. siamea</em>, <em>C. fistula</em>, <em>C. surattensis</em>, and <em>C. spectabilis</em>). Phytochemical screening of the extract was done based on the Harborne method. Evaluation of the anthelmintic activities against <em>H</em>. <em>contortus </em>was done <em>in vitro </em>using infective larvae (L<sub>3</sub>) migration inhibition assay (LMIA). Measurement of larvae migrating was conducted through a nylon filter with a pore size of 20 μm. The doses of <em>Cassia </em>spp. extract implemented were 25, 50, 100, and 200 mg/ml.</p> <p><strong>Results: </strong>Tannins, alkaloids, phenol hydroquinone, flavonoids, steroids, triterpenoids, and sapo­nins were present in all the extracts, whereas alkaloids were absent in <em>C</em>. <em>fistula</em>. No triterpenoids were found in <em>C. surattensis </em>and <em>C. spectabilis</em>. Movement of <em>H</em>. <em>contortus </em>larvae was significantly inhibited after exposure to <em>Cassia </em>extracts at various dosage levels (<em>p </em>< 0.05). The test results using LMIA on L<sub>3</sub> <em>H</em>. <em>contortus </em>showed the lowest inhibition in the negative control. Among the species of <em>Cassia</em>, the <em>C</em>. <em>surattensis </em>(at 200 mg/ml) showed the highest (<em>p </em>< 0.05) inhibition level on the larvae. The latter result corresponded to the effect of albendazole.</p> <p><strong>Conclusion: </strong>Compared to other <em>Cassia </em>spp., <em>C</em>. <em>surattensis </em>exhibited the highest inhibition against L3 <em>H</em>. <em>contortus</em>. However, the inhibition effect of <em>C</em>. <em>surattensis </em>was still lower as compared to albendazole.</p> <p>J. Adv. Vet. Anim. Res., 6(2): 236-240, June 2019</p>}, number={2}, journal={Journal of Advanced Veterinary and Animal Research}, author={Wahyuni, Sri and Sunarso, Sunarso and Prasetiyono, Bambang Waluyo Hadi Eko and Satrija, Fadjar}, year={2019}, month={Jun.}, pages={236–240} }