@article{Puja_Sawitri_Maharani_Heryani_Dharmayudha_Gunawan_2019, title={Preservation of semen from Kintamani Bali dogs by freezing method}, volume={6}, url={https://www.banglajol.info/index.php/JAVAR/article/view/41747}, abstractNote={<p><strong>Objective: </strong>To explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa.</p> <p><strong>Materials and Methods: </strong>Sample was collected from four mature Kintamani Bali dogs. Each ejacu­late was prepared for cryopreservation with two different semen extenders; egg yolk Tris extender and coconut water-based extender. For each extender, three different glycerol concentrations were used; 4%, 6%, and 8%. Each of the six aliquots was loaded into 0.5 ml cryotube, placed on a styrofoam box 5 cm over liquid nitrogen for 10 min, and immersed in liquid nitrogen up to 8 min. Then, the frozen cryotubes were transferred into liquid nitrogen container. The cryotubes were thawed in a water bath at 38.5°C for 120 sec. After equilibration and thawing, each sample was assessed for motility parameters and for DNA fragmentation.</p> <p><strong>Results: </strong>The addition of 6% glycerol to extenders revealed the most effective addition of glycerol on motility and sperm DNA fragmentation after equilibrium and post-thawing.</p> <p><strong>Conclusion: </strong>It is concluded that both extenders with the addition of 6% glycerol are safe to be used as an extender in Kintamani Bali dog semen preservation, and DNA fragmentation of Kintamani Bali dog spermatozoa was not influenced by the freezing procedure.</p> <p>J. Adv. Vet. Anim. Res., 6(2): 158-162, June 2019</p>}, number={2}, journal={Journal of Advanced Veterinary and Animal Research}, author={Puja, I Ketut and Sawitri, Ni Made and Maharani, Nisa and Heryani, Luh Gde Sri Surya and Dharmayudha, Anak Agung Gde Oka and Gunawan, I Wayan ico Fajar}, year={2019}, month={Jun.}, pages={158–162} }