@article{Kisworo_Depamede_2018, title={Antibody against synthetic peptide of prolactin-inducible protein homologue precursor (PIP-HP) of Bali Cattle (Bos javanicus) saliva as a potential biomarker for immunoassay development}, volume={5}, url={https://www.banglajol.info/index.php/JAVAR/article/view/37164}, abstractNote={<p><strong>Objective</strong>: The present study aimed to produce and analyze antibody against a synthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva.</p><p><strong>Materials and Methods</strong>: The synthetic amino acid sequence of the PIP-HP (VIRELGICPDDWAVIPIKANRF) was developed, conjugated to bovine serum albumin and was used to immunize Indonesian local rabbits. Positive sera that specific against the PIP-HP were pooled and purified sequentially by implying ammonium sulfate precipitation and protein A affinity methods. Purified antibody was then employed to analyze of PIP-HP in the ruminants’ saliva by means of westernblot assays.</p><p><strong>Results</strong>: A polyclonal antibody specific to asynthetic amino acid sequence fragment of PIP-HP of Bali cattle saliva was successfully produced. Our results show that the antibody potentially to be used to develop an immuno-diagnostic kit. Furthermore, the antibody was also able to inhibit the growth of both <em>Escherichia coli </em>and <em>Staphylococcus aureus </em>cultures significantly (<em>P</em><0.001) at concentration of 0.3 mg/mL.</p><p><strong>Conclusions</strong>: In this study, antibody against synthetic peptide of PIP-HP (VIRELGICPDDWAVIPIKANRF) of Bali cattle saliva origin has been produced successfully. Further studies need to be carried out since the antibody has potential as biomarker for non-invasive immunoassays development.</p><p><span><a href="http://doi.org/10.5455/javar.2018.e264">http://doi.org/10.5455/javar.2018.e264</a>     </span></p>}, number={2}, journal={Journal of Advanced Veterinary and Animal Research}, author={Kisworo, Djoko and Depamede, Sulaiman Ngongu}, year={2018}, month={Jun.}, pages={182–187} }