TY - JOUR AU - Nallamuthu, Ilaiyaraja AU - Parthasarathi, Ambica AU - Khanum, Farhath PY - 2013/11/14 Y2 - 2024/03/29 TI - Thymoquinone-loaded PLGA nanoparticles: antioxidant and anti-microbial properties JF - International Current Pharmaceutical Journal JA - Int Curr Pharm J VL - 2 IS - 12 SE - Original Articles DO - 10.3329/icpj.v2i12.17017 UR - https://www.banglajol.info/index.php/ICPJ/article/view/17017 SP - 202-207 AB - <p>The aim of the present study was to synthesize and characterize the Thymoquinone (TQ) encapsulated PLGA (poly (dl-lactide-co-glycolide) nanoparticles, and further evaluate for its antioxidant and anti-bacterial activities. TQ is a potential active ingredient of <em>Nigella sativa</em> seed and possess a spectrum of therapeutic properties. Nanoparticles were prepared according to solid-in-oil-in-water (s/o/w) solvent evaporation method. Dynamic laser light scattering (DLS) and SEM studies indicated a mean particle size of &lt; 200 nm. The success of encapsulation was confirmed by FTIR technique, and the encapsulation efficiency (EE) of TQ was determined to be 62%. <em>In vitro</em> drug release study showed a maximum release of TQ at 75% and 54 % respectively for artificial intestinal and gastric juices over the period of 7 days. DPPH radical scavenging activity of the nanoparticles was found to be 71% at 1 mg/ml concentration. It also exhibited antibacterial property against <em>E. coli, Staphylococcus aureus </em>and<em> Salmonella typhi</em> strains, tested using well diffusion method. In conclusion, our study shows that PLGA encapsulated TQ nanoparticle with sustained release property has preserved antioxidant as well as anti-microbial activity, and therefore suggesting its therapeutic applications in various food samples.<strong></strong></p><p>DOI: <a href="http://dx.doi.org/10.3329/icpj.v2i12.17017">http://dx.doi.org/10.3329/icpj.v2i12.17017</a></p> International Current Pharmaceutical Journal, November 2013, 2(12): 202-207 ER -