Validated stability indicating gradient RP-HPLC method for the estimation of antihypertensive drugs in bulk and pharmaceutical dosage forms
DOI:
https://doi.org/10.3329/icpj.v1i11.12058Keywords:
Olmesartan medoxomil, Hydrochlorothiazide, INERTSIL ODS C18, Orthophosphoric acid, Acetonitrile, ICH GuidelinesAbstract
A rapid and precise RP-HPLC method for determination of Olmesartan medoxomil and Hydrochlorothiazide in bulk and pharmaceutical dosage forms. Olmesartan medoxomil & Hydrochlorothiazide are found to be degraded together under different set of conditions as followed according to ICH guidelines and the degradants so formed along with olmesartan & hydrochlorothiazide are separated by using INERTSIL ODS C18 3V (150 x 4.6, 5µ) using mobile phase 1ml triethanolamine in one litre water and the pH was adjusted to 2.5 with orthophosphoric acid and acetonitrile using a gradient program with a flow rate of 1ml/min, throughout the gradient program with a detection wavelength of 225nm for both the compounds with a injection volume of 10µl. The method was validated for selectivity, linearity, accuracy, robustness, precision and specificity. The results were indicating the method was selective in analysis of both olmesartan medoxomil and hydrochlorothiazide in the presence of degradation products formed under various stress conditions.
DOI: http://dx.doi.org/10.3329/icpj.v1i11.12058
International Current Pharmaceutical Journal 2012, 1(11): 336-341
Downloads
152
141
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- The journal holds copyright and publishes the work under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).