Structure Elucidation of Soluble Dietary Fiber from Cauliflower

  • Nilufar Nahar Department of Chemistry, Dhaka University, Dhaka-1000
  • AKM Ahsan Habib Department of Chemistry, Dhaka University, Dhaka-1000
  • SM Mizanur Rahman Department of Chemistry, Dhaka University, Dhaka-1000
  • Tofail Ahmed Chowdhury Department of Chemistry, Dhaka University, Dhaka-1000
Keywords: Soluble Dietary Fiber, Cauliflower, Arabinogalactan, Rhamnogalactglucuronan

Abstract

Arabinogalactan and rhamnogalactglucuronan were isolated from soluble dietary fiber (SDF) of cauliflower. The isolated SDF was fractioned into neutral and acidic parts by ion-exchange column chromatography using DEAESepharose Cl-6B gel. Structure elucidation of the isolated SDF polysaccharides were determined sugar analysis, 1HNMR, H-H COSY and TOCSY spectroscopic studies. Neutral fraction of cauliflower was found to be composed of galactose, arabinose and rhamnose whereas the acidic part was found to contain rhamnose, galactose, galacturonic and glucuronic acids. The main chain of the neutral fraction was found to be a 1,4-?-galactan where terminal rhamnose and arabinose residues were attached to the main chain at the 3-position of by ?-linkage.The main chain of the acidic fraction was composed of 1,4-b-linked galacturonic acid and 1,2-b-linked rhamnopyranose residues. ?- Glucuronic acid and ?-galactose were attached to the main chain of galacturonic acid at its 3-position.

Dhaka Univ. J. Sci. 64(1): 83-88, 2016 (January)

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Author Biography

Nilufar Nahar, Department of Chemistry, Dhaka University, Dhaka-1000


Published
2016-06-28
How to Cite
Nahar, N., Habib, A., Rahman, S., & Chowdhury, T. (2016). Structure Elucidation of Soluble Dietary Fiber from Cauliflower. Dhaka University Journal of Science, 64(1), 83-88. https://doi.org/10.3329/dujs.v64i1.28529
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Articles