TY - JOUR AU - Rabbane, Md Golam AU - Ahmed, Md Feroz AU - Alam, Mohammad Shamimul AU - Hossain, Md Motaher PY - 2016/07/20 Y2 - 2024/03/28 TI - Culture, reproduction and embryogenesis of wild zebrafish (Danio rerio) in laboratory condition JF - Dhaka University Journal of Biological Sciences JA - Dhaka Univ. J. Biol. Sci VL - 25 IS - 2 SE - Articles DO - 10.3329/dujbs.v25i2.46336 UR - https://www.banglajol.info/index.php/DUJBS/article/view/46336 SP - 139-148 AB - <p>Zebrafish (<em>Danio rerio</em>: Hamilton, 1822) is a well-known model organism of biological research. Generally it is found in the fresh water ponds. However, the culture, breeding and embryonic development of wild <em>D. rerio </em>of Bangladesh is scarcely represented in the literature. Therefore, a laboratory experiment was conducted to study these aspects of local zebrafish. Water quality parameters for adult fish husbandry system were recorded. These were temperature (<sup>°</sup>C) 29.18 ± 1.53, pH 7.55 ± 0.144, TDS (ppm) 225 ± 11.136, (ORP) 48.93 ± 26.04, DO (ppm) 1.24 ± 0.591, conductivity (mS/cm) 0.450 ± 0.022, are resistivity (Mῼcm) 0.0026 ± 0.00049 for adult fish tanks. The parameters obtained from larvae rearing ranks were temperature (<sup>°</sup>C) 29.56 ± 0.777, pH 7.73 ± 0.421, TDS (ppm) 198.33 ± 39.627, (ORP) 51.37 ± 17.387, DO (ppm) 2.02 ± 0.694, conductivity (mS/cm) 0.397 ± 0.079, resistivity (MΩcm) 0.0026 ± 0.00044. After one month of rearing, adult male and female fishes were selected for natural breeding and spawning and obtained 87.5 ± 7.72% spawning success, 96.83±0.81% fertilization rate and 94.89 ± 0.27% hatching rate. The embryonic development for zygote, cleavage, blastula, gastrula, segmentation and pharyngula periods were observed and obtained proper development for each state at 28.5<sup>º</sup>C. The survival rate of hatchlings was 10 - 20% up to 20 days post fertilization. The higher spawning success, fertilization rate, hatching rate and survival rate indicate the possibility and potentiality of culturing zebrafish in laboratory conditions and could be established as a model animal for Bangladeshi researchers.</p><p>Dhaka Univ. J. Biol. Sci. <strong>25</strong>(2): 139-148, 2016 (July)</p> ER -