Effect of Vitamin E on Serum Urea Level on Gentamicin Induced Nephrotoxicity in Long Evans Rats

  • Md Shameem Ahmed Professor and Head, Dept. of Anatomy, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
  • Mohammad Ashraf Ahmed Assistant Professor, Dept. of Pharmacology, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
  • Md Rezwanur Rahman Professor, Dept. of Biochemistry, Delta Medical College, Dhaka, Bangladesh
  • - Ashrafuzzaman Professor and Head, Dept. of Anatomy, Chittagong Medical College, Chittagong, Bangladesh
  • Mushtari Jahan Dental Surgeon, Dhaka, Bangladesh
  • Md Abdul Matin Associate Professor, Dept. of Paediatrics, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
  • Aysha Yasmin Associate Professor and Head, Dept. of Physiology, KhwajaYunus Ali Medical College, Enayetpur, Sirajgonj, Bangladesh
  • Marjina Khatun Assistant Professor, Dept. of Community Medicine, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
  • Farhana Afroz Assistant Professor, Dept. of Paediatrics, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
  • Kuazi Dil Afroz Associate Professor, Dept. of Pathology, Kumudini Women’s Medical College, Mirzapur,Tangail, Bangladesh
Keywords: Gentamicin; vitamin E nephrotoxicity

Abstract

Background: The kidneys have an important role in eliminating the final products of metabolic activities, excreting the drugs and chemicals. A variety of frequently used drugs have been demonstrated to produce nephrotoxic effects.

Objective: This study was carried out to observe the effect of vitamin E on gentamicin-induced nephrotoxicity by assessing serum urea level in Long Evans rats.

Materials and method: The experimental study was carried out on 40 healthy Long Evans rats of both sex with the weight ranges from 172-255 gm and the age ranges from 7 to 10 weeks. The rats were divided into four groups - Group A (normal control) received normal saline, group B, C and D received gentamicin for 6 days, rats of group C received vitamin E capsule for total 9 days with gentamicin whereas group D received vitamin E capsule for total 10 days with gentamicin. Serum urea level was measured at the end of the experiment.

Results: The (mean±SD) serum urea levels in group A, B, C and D were 4.79±0.32, 12.41±1.22, 7.56±1.11 and 7.15±1.09 mmol/L respectively. The differences between groups were highly significant (p<0.001) for group A & B, A & C, A & D, B & C, B & D whereas the difference between C & D (p>0.01) was not significant. Serum urea level of the normal saline control group (group A) was within the normal limit (4.79 mmol/L). Serum urea level in gentamicin treated rats (group B) was more in comparison to gentamicin and vitamin E treated rats (group C & D) and pretreatment with longer duration group (group D) showed lower serum urea value than shorter one (group C) though the groups showed no significant difference.

Conclusion: Vitamin E treatment showed some protective effect against gentamicin-induced nephrotoxicity. The results also indicated that effectiveness of vitamin E depends on duration of pretreatment that means the pretreatment duration must be increased to a suitable period for better protection against gentamicin-induced nephrotoxicity.

Delta Med Col J. Jan 2019 7(1): 11-15

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Abstract
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PDF
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Published
2019-03-19
How to Cite
Ahmed, M. S., Ahmed, M. A., Rahman, M. R., Ashrafuzzaman, -, Jahan, M., Matin, M. A., Yasmin, A., Khatun, M., Afroz, F., & Afroz, K. (2019). Effect of Vitamin E on Serum Urea Level on Gentamicin Induced Nephrotoxicity in Long Evans Rats. Delta Medical College Journal, 7(1), 11-15. https://doi.org/10.3329/dmcj.v7i1.40613
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Original Articles