Association of serum vitamin D 3 with newly diagnosed type 2 diabetes mellitus

Vitamin D has an important role and supposed to be a risk factor in the development of pathogenesis of type 2 diabetes mellitus by affecting either insulin sensitivity or β-cell function, or both. The present study was conducted to evaluate the relation of serum vitamin D 3  and type 2 diabetes mellitus. Total 80 individuals were enrolled in this study. Forty newly diagnosed type 2 diabetic individual were enrolled as cases and 40 healthy glucose tolerant subjects were enrolled as controls. Serum vitamin D 3 was measured by chemiluminescence microparticle immunoassay. Fasting and 2 hours plasma glucose level were measured by hexokinase method using the Dimension clinical chemistry system. The mean serum vitamin D 3 level was significantly low in type 2 diabetes mellitus than in controls (p= 0,007). The individual with vitamin D 3 deficiency showed 3.4 times higher chances to develop type 2 diabetes mellitus compare to the individual with normal vitamin D status. Correlation test showed significant (r= -0.351) negative correlation (p=0.026)  between serum vitamin D 3 and type 2 diabetes mellitus. It can be concluded that vitamin D 3 deficiency is related to type 2 diabetes mellitus.


Introduction
Type 2 diabetes mellitus is a metabolic disorder that has become increasingly prevalent in the developing world. 1 The exponential increase is a great cause for concern as it is no longer a disease found solely in adults, but has become increasingly evident in children as well. 2 Vitamin D deficiency has been related to multiple types of health problems including different cancer, autoimmune and metabolic diseases like type 1 and 2 diabetes mellitus. 3 addition to genetics which predisposes individuals to develop type 2 diabetes mellitus, there are also many environmental factors which contribute greatly to its development like physical inactivity, poor nutrition and obesity.Recent evidence suggests that vitamin D deficiency (as measured by serum 25-hydroxyvitamin D3 concentration) may also contribute to the pathogenesis of type 2 diabetes mellitus. 4e pathogenesis of type 2 diabetes mellitus is quite complex.It involves many different pathways, organs, tissues and hormones. 5 keeping with the notion that type 2 diabetes mellitus cannot manifest without -cell failure.It is important to examine the role of vitamin D metabolites (1,25(OH)2D3) in pancreatic -cell function.The 1a(OH)ase enzyme, although originally thought to be present only in the renal tissue, has been identified also in the extra -renal tissues like pancreatic -cells. 6The identification of the 1a(OH)ase in the -cells suggests that 1,25(OH)2D3 may play a role in the overall -cell function.In vitro and in vivo studies have ascertained that 1, 25(OH)2D3 is necessary for insulin secretion and glucose homeostasis. 7These reports suggest that vitamin D deficiency affects the -cell function and that vitamin D supplementation improves the -cell function. 8rrently, the incidence of hypovitaminosis D is high.It has long been suspected as a risk factor for glucose intolerance. 9So, this study was conducted to know the association of serum vitamin D3 deficiency and type 2 diabetes mellitus.

Materials and Methods
This case-control study was conducted from January to December 2013.It was a convenient and purposive sampling.Forty newly diagnosed type 2 diabetic individuals of both sexes, age between 30 to 60 years, attending in the sample collection booth of the department were enrolled and 40 healthy glucose tolerant subjects were selected from the university

Abstract
Vitamin D has an important role and supposed to be a risk factor in the development of pathogenesis of type 2 diabetes mellitus by affecting either insulin sensitivity or -cell function, or both.The present study was conducted to evaluate the relation of serum vitamin D3 and type 2 diabetes mellitus.Total 80 individuals were enrolled in this study.Forty newly diagnosed type 2 diabetic individual were enrolled as cases and 40 healthy glucose tolerant subjects were enrolled as controls.Serum vitamin D3 was measured by chemiluminescence microparticle immunoassay.Fasting and 2 hours plasma glucose level were measured by hexokinase method using the Dimension clinical chemistry system.The mean serum vitamin D3 level was significantly low in type 2 diabetes mellitus than in controls (p=0.007).The individual with vitamin D3 deficiency showed 3.4 times higher chances to develop type 2 diabetes mellitus compare to the individual with normal vitamin D status.Correlation test showed significant (r= -0.351) negative correlation (p=0.026) between serum vitamin D3 and type 2 diabetes mellitus.It can be concluded that vitamin D3 deficiency is related to type 2 diabetes mellitus.; : -.

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Statistical analysis
The statistical analysis was done using the software SPSS.The results were expressed as mean ± SD (Standard Deviation) and median (range).Comparison was done by unpaired t-test, X 2 test and Pearson's correlation test.The p value of <0.05 was considered statistically significant.

Results
The mean ± SD of serum vitamin D concentration in the newly diagnosed type 2 diabetes mellitus was 17.7 ± 4.5 ng/mL with the range of 8-28.2 ng/mL and in non-diabetic controls were 21.6 ± 7.7 ng/mL with the range of 13.6-49.2ng/mL.Serum vitamin D concentration was found to be significantly low (p=0.007) in type 2 diabetic cases as compared to non-diabetic controls (Table I).
The prevalence of hypovitaminosis D in newly diagnosed type 2 diabetes mellitus was found to be more (27.5%)as compared to non-diabetic controls (10%).Hypovitaminosis D was significantly high (p=0.045) in type 2 diabetes mellitus than the normal glucose tolerant individuals.

Discussion
Vitamin D3 deficiency is an increasingly recognized health concern related to skeletal and non-skeletal outcomes.Recent studies have found a relationship between vitamin D3 status and diabetes and may be a risk factor in the pathogenesis of type 2 diabetes mellitus by affecting either insulin sensitivity orcell function, or both. 12 this study, the mean serum vitamin D level was significantly lower in type 2 diabetes than in controls.Tracy and Mazen (2010) 13 found that Vitamin D3 deficiency has been linked to type 2 diabetes mellitus, with circulating levels of 25(OH)D3 significantly (p<0.008)lower in type 2 diabetics than in controls, although both groups had mean values well below the level considered ideal.This finding conforms to other similar studies done by Targher  et al. (2006), 14 Sung et al. (2012), 12  Chiu et al. (2004). 8povitaminosis D was found to be higher in diabetic patients (27.5%) than in controls (10%).Our study finding is consistent with the observation of Targher et al. (2006). 14tamin D3 deficiency has been suspected as a risk factor for impaired glucose tolerance and diabetes among adults.In the current study, we found that plasma 25(OH)D3 concentrations were inversely associated with fasting glucose.The inverse association between plasma 25(OH)D3 and fasting glucose is consistent with previous epidemiologic studies done by Targher et al. (2006), 14

Conclusion
Vitamin D3 deficiency is inversely correlated with fasting blood glucose thus associated with type 2 diabetes mellitus.The individual with vitamin D3 deficiency showed 3.4 times high-risk to develop type 2 diabetes mellitus compare to the individual with normal vitamin D status.
opyright of this arti le is retai ed y the author s [Atri utio CC-By .] Availa le at: .a glajol.ifo A Jour al of Ba ga a dhu Sheikh Muji Medi al U i ersity, Dhaka, Ba gladesh Association of serum vitamin D3 with newly diagnosed type 2 diabetes mellitus Taznuva Anwar, Md.Matiur Rahman, Forhadul Hoque Mollah and Subrata Kumar Biswas employees, colleagues and patients' attendants of both sex, age between 30 to 60 years were enrolled as controls.The blood sample was collected from each study subject with all aseptic precaution.Fasting plasma glucose and 2 hours plasma glucose were measured by hexokinase method using the Dimension clinical chemistry system. 10Serum vitamin D3 was estimated by chemiluminescence m i c r o p a r t i c l e i m m u n o a s s a y ( C i 4 1 0 0 ARCHITECT). 11Hypovitaminosis D was defined as a serum 25(OH)D level below 20 ng/mL.

Table I Comparison of vitamin D3 status between case and control
8igure 1: Correlation of vitamin D with fasting blood glucose in type 2 diabetes mellitus blood glucose in our study.Chiu et al. (2004)8found that circulating 25(OH)D3 concentrations were inversely related to 2 hours plasma glucose in oral glucose tolerance test which is inconsistent with the current study.However, inconsistent results between the studies may be attributed to different populations studied and different time points of glucose measurement during oral glucose tolerance test.