Bangladesh Rice Journal <p>Official journal of Bangladesh Rice Research Institute (BRRI). Full text articles available.</p> en-US <p class="ListParagraph">All rights reserved to Executive Editor, Bangladesh Rice Journal (BRJ), BRRI, Gazipur-1701.</p> (Dr. Md. Shahjahan Kabir) (Md Fahmid Uddin Khondoker) Thu, 30 Jul 2020 09:23:04 +0000 OJS 60 Molecular and Phenotypic Characterization for Cold Tolerance in Rice (Oryza sativa L.) <p>Cold stress affects growth and development of rice plants from germination to reproductive stages. Thirty breeding lines/varieties including tolerant and susceptible check varieties were evaluated for cold tolerance at seedling and reproductive stage using artificially induced low temperature situations to understand the genetic relationship among them and to identify promising donors for cold tolerance. Three breeding lines, BR8253-9-3-4-3, IR77497-31-2-3-1and IR2266-42-6-2 demonstrated higher cold tolerance for the seedling stage (LD score &lt;3.0). Molecular analysis revealed 68% genetic similarity among the genotypes. On average 64% of the breeding lines shared common alleles at any given locus ranging from 27% (RM22870) to 97% (RM186). UPGMA dendrogram showed that majority of the breeding lines showing 4-5 degrees of LD were grouped together. Two breeding lines, BR8258-7-1-5-2B2 and BR8258-7-1-5-2B3 having 86% common alleles showed low leaf discoloration (LD: 4) at seedling stage coupled with relatively low reduction in spikelet fertility (45.8% and 48.1%) and grain yield (43.3% and 39.1%). The gene diversity and PIC value revealed that seven SSR loci (RM1282, RM600, RM5349, RM6972, RM5847, RM5911, and RM511) might be the best markers to differentiate between tolerant and susceptible genotypes at reproductive stage. The breeding lines with tolerance to cold stress at the seedling and the reproductive stages identified from this study, and the SSR markers mentioned above could be useful for developing cold tolerance rice.</p> <p>Bangladesh Rice j. 2019, 23(2): 1-15</p> PS Biswas, H Khatun, M Anisuzzaman ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Response to Callus Induction and Regeneration of Newly Released BRRI Rice Varieties <p>A study was carried out for developing an efficient callus induction and regeneration system for three newly developed BRRI varieties namely BRRI dhan86, BRRI dhan87 and BRRI dhan89. Dehusked seeds were plated onto MS and N6 media with two hormone combinations for callus induction. Calli obtained from each callus induction medium were transferred to four different regeneration media. Callus induction frequency and regeneration ability were significantly influenced by rice varieties, and interactions of variety and media. Among the media compositions, the highest callus (59.44%) were obtained from C<sub>1</sub> (MS+2mg/l 2,4-D) followed by C<sub>2</sub> ( MS+2 mg/l 2,4-D+0.5 mg/l kinetin) , C<sub>3</sub> ( N6+2 mg/l 2,4-D) and C<sub>4</sub> (N6+2 mg/l 2,4-D+0.5 mg/l kinetin) medium. The highest regeneration (45.74%) was obtained from R<sub>2</sub> (MS+4 mg/ml BAP+1.2 mg/ml kinetin+0.5 mg/ml NAA), followed by R<sub>3</sub> (1 mg/ml BAP+1 mg/ml Kinetin+1 mg/ml NAA), R<sub>4</sub> (2 mg/ml kinetin+1 mg/ml NAA+300 mg casein hydrolysate) and R<sub>1</sub> (2 mg/ml BAP+1 mg/ml kinetin+1 mg/ml NAA). BRRI dhan86 showed the highest regeneration ability (53.06%) than the other two varieties. It is observed that all varieties performed better in C<sub>1</sub> medium for callus induction and R<sub>2</sub> medium for regeneration. This study also revealed that BRRI dhan86 was more responsive to callus induction and regeneration of green plants than the other two varieties.</p> <p>Bangladesh Rice j. 2019, 23(2): 17-25</p> SD Joya, S Sultana, J Ferdous, MA Qayum, ME Hoque ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Genetic Variability, Heritability, Correlation and Path Coefficient Studies for Yield and Yield Components of Some Promising Rice Hybrids <p>Eight promising hybrids along with three checks were evaluated for yield and yield contributing traits to observe their genetic variability, heritability, correlation and path coefficient analysis during T. Aman season 2014. The results indicated that the highest genotypic variance was recorded in spikelet panicle<sup>-1</sup> followed by effective tiller m<sup>-2</sup>. Similarly, the highest phenotypic variances were also found with these two characters. Phenotypic coefficient of variation (PCV) was slightly higher than genotypic coefficient of variation (GCV) for all the traits under this study. Hence, slight differences indicate less or minor environmental influence and greater role of genetic factors on the expression of the traits. High heritability was observed in all the characters studied except effective tiller m<sup>-2</sup>. Highly significant and positive correlations of grain yield with effective tiller m<sup>-2</sup>, spikelet panicle<sup>-1</sup> at genotypic level were observed. Spikelet fertility was found significant at both genotypic and phenotypic level. Path analysis revealed that spikelet fertility had highly positive direct effect on grain yield followed by effective tiller m<sup>-2</sup>. On the other hand, spikelet panicle<sup>-1</sup> showed positive indirect effect on grain yield. Thus, the results suggested that effective tiller m<sup>-2</sup>; number of spikelet panicle-1 and spikelet fertility (%) could be considered as effective selection criteria for the development of heterotic rice hybrids.</p> <p>Bangladesh Rice j. 2019, 23(2): 27-34</p> A Akter, MJ Hasan, MA Latif, MU Kulsum, PL Biswas, MH Rahman, RR Majumder, LF Lipi, MR Quddus, F Akter, A Ara ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Adaptation of Promising Rice Genotypes for Broadcast Aus Season <p>Rice cultivation in Aus season is eco-friendly and essential for sustainable food security of Bangladesh utilizing rain water rather than extracting underground water. Four rice genotypes (BR6855-3B-12, BR6855-3B-13, BR6848-3B-12 and BR6976-2B-11-1) along with check variety BRRI dhan43 were evaluated at farmers’ field during broadcast Aus season. The objectives were to investigate the adaptability of the rice genotypes and find out promising genotype(s) for Broadcast Aus rice. The experiment was conducted at farmers’ field in eight agro-ecological zones of Bangladesh. Randomized complete block design (RCB) was used with three replications. Standard and uniform management practices were followed for all the locations. Data were taken on grain yield, days to 80% maturity, plant height, panicles m<sup>-2</sup>, 1000-grain weight, grains panicle<sup>-1</sup>, spikelet sterility (%), Phenotypic acceptance at vegetative and maturity stage, pest incidence; feedback of farmers and extension personnel. Genotypes, environments and interaction of genotypes by environments had significant effect on grain yield, yield components and agronomic parameters. Analysis of variance indicated that there were significant differences between the check (control) and improved genotypes except the genotype BR6976-2B-11-1 which was similar with the check BRRI dhan43 for all parameters. Correlation analysis was also performed to establish extent of association between yield and major yield components. Grain yield positively and significantly correlated with the agronomic parameters except spikelet sterility. Across the locations, BR6848-3B-12 produced significantly the highest grain yield (4.88 t ha<sup>-1</sup>) at Feni while the lowest grain yield (1.58 t ha<sup>-1</sup>) was obtained in BR6976-2B-11-1 followed by BRRI dhan43 (1.64 t ha<sup>-1</sup>) at Kushtia. BR6848-3B-12 showed yield advantage of 1.0 t ha<sup>-1</sup> over the check variety BRRI dhan43 with similar growth duration (104 day). AMMI stability value, yield stability index and GGE Bi-plot model indicated that BR6855-3B-12 is the most adaptable, suitable and stable genotype for broadcast Aus rice across the locations, and finally it is released in 2017 as BRRI dhan83 for broadcast Aus season.</p> <p>Bangladesh Rice j. 2019, 23(2): 35-48</p> B Karmakar, MAA Mamun, MS Rahman, MA Islam, MR Islam, MHR Mukul, - Shamsunnaher, A Zahan, R Barua, MR Biswash, S Parveen, S Akter, NY Shaikh, B Ahmed ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Effect of Nitrogen Rates and Leaf Clipping on Forage and Grain Yield, and Seed Quality of Transplant Aman Rice <p>Sustainable fodder production is difficult from limited cultivable land occupied for food grain production. This paper presents the results of the experiment designed to test the hypothesis that forage could be produced along rice grain from the same rice field. The aim of the present study was to investigate the influence of N-rates and leaf clipping on forage and grain yield; and seed quality of transplant Aman (wet season) rice. Four nitrogen (N) rates (N<sub>1</sub>=46, N<sub>2</sub>=69, N<sub>3</sub>=92 and N<sub>4</sub>=115 kg N ha<sup>-1</sup>) and four times of leaf clipping viz, C<sub>0</sub>=No leaf clipping, C<sub>1</sub>=leaf clipping at 25 DAT (Days after transplanting), C<sub>2</sub>=40 and C<sub>3</sub>=55 DAT were evaluated following split-plot design with three replications. BRRI dhan41 was used in the experiment. N rates, leaf clipping times and its interaction had significant effects on forage and grain yield, yield components and seed quality except thousand grain weight. The results revealed that forage yield increased with increasing N rates and leaf clipping times. Grain yield was higher in higher N rates and early leaf clippings, however, it was decreased in the late clipping (55 DAT). The highest mean grain yield (5.25 t ha<sup>-1</sup>) was obtained from the treatment combination of 115 kg N ha<sup>-1</sup> (N4) and no leaf clipping (N<sub>4</sub>C<sub>0</sub>) which was statistically similar (5.18 t ha<sup>-1</sup>) to N<sub>3</sub>C<sub>0</sub> (5.18 t ha<sup>-1</sup>), N<sub>3</sub>C<sub>1</sub> (5.10 t ha<sup>-1</sup>) and N<sub>4</sub>C<sub>1</sub> (5.06 t ha<sup>-1</sup>). The lowest mean grain yield (3.17 t ha<sup>-1</sup>) was obtained from N<sub>1</sub>C<sub>3</sub>. Grain yield was reduced by 3, 6 and 24% in C<sub>1</sub>, C<sub>2</sub> and C<sub>3</sub>, respectively compared to C<sub>0</sub> (No leaf clipping). Qualitative characters (germination and vigour index) of seed increased with increased N rates, however it decreased in the delayed leaf cuttings. Application of 92 to 115 kg N ha<sup>-1</sup> with early leaf clipping (25 to 40 DAT) might be allowed to attain moderate forage yield, higher grain yield, and quality seed of transplant Aman rice.</p> <p>Bangladesh Rice j. 2019, 23(2): 49-57</p> Banani Karmaker, Biswajit Karmakar ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Genetic Diversity of Bacterial Blight Resistant Rice (Oryza sativa L.) Genotypes from INGER <p>Genetic diversity of 65 rice genotypes was studied from IRBBN (International Rice Bacterial Blight Nursery) of INGER (International Network for Genetic Evaluation of Rice) materials through Mahalanobis D2 statistic for grain yield and yield contributing characters. The genotypes were grouped into five clusters. The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the clusters. The cluster III contained the highest number of genotypes (23) and the clusterv contained the lowest (8). The highest intra-cluster distance was noticed for the cluster I and lowest for cluster III. The highest inter-cluster distance was observed between cluster I and V, followed by cluster IV and V, cluster II andV and the lowest between cluster I and IV. Regarding inter-cluster distance, the genotypes of cluster V showed high genetic distance from all other clusters. The genotypes from cluster V could be hybridized with the genotypes of other clusters for producing transgressive segregants. Based on canonical vector analysis, panicle number per plant had maximum contribution towards genetic divergence. The highest cluster means for yield, grain/panicle and spikelet fertility were obtained from cluster V. The highest means for 1000 grain weight, second higher yield and the lowest growth duration were found in cluster II, while the lowest mean value for yield and 1000 grain weight and higher mean value for growth duration were found in cluster IV. The crosses between the genotypes/parents of cluster V and cluster II, cluster V and cluster I would exhibit high heterosis as well as higher level of yield potential. Therefore, more emphasis should be given for selection of the genotypes from clusters II and V for future breeding programme.</p> <p>Bangladesh Rice j. 2019, 23(2): 59-64</p> F Akter, MZ Islam, A Akter, SK Debsharma, A Shama, M Khatun ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Custom Hire Service Business of Rice Combine Harvester in Haor Basin of Bangladesh <p>Mechanization is a vital issue to modernize agriculture. The entrepreneurship development is imperative to provide service to the farmers at an affordable price, especially in <em>haor </em>areas. The study was conceived to estimate the combine harvester's rental charge and payback period. Data were collected from 86 rice fields harvested by a whole-feed combine harvester (Model: Zoomlion) in Mithamain upazila under the Kishoreganj district representing <em>haor </em>area of Bangladesh. Land size, operational time, loss time, repair time, idle time, daily area coverage and constraints of harvester machine were also recorded to predict the business viability of combine harvester. The rental charge and payback period was calculated following standard protocol to make business venture profitable. The perception of farmers regarding the prospect of using combine harvester in harvesting has been recorded through personal interviews. The field capacity of combine harvester was observed as 0.20 ha hr<sup>-1</sup>. Field efficiency of combine harvester depended on the land size. The daily area coverage can be increased after careful selection of the plots. Fuel consumption was found 4.18 l ha<sup>-1</sup>. Operational consolidation is the best approach to reduce the time of movement resulting in increased daily area coverage. Seasonal use of combine harvester in one locality of the study area was observed for 22 days. The rental charge of the machine was estimated as 10,000 Tk ha<sup>-1</sup> and payback period was 3.5 years for the area coverage of 40 ha rice fields. Farmers would be able to save 40% harvesting cost for the rental charge of Tk 10,000 ha<sup>-1</sup>. Combine harvester rental service has emerged as a viable business model in the <em>haor </em>basin. Whole-feed (Zoomlion model) combine harvester appeared as an effective, economical, and labour-saving harvesting machine in the <em>haor </em>region land tenure system.</p> <p>Bangladesh Rice j. 2019, 23(2): 65-75</p> AKMS Islam, MGK Bhuiyan, M Kamruzzaman, MA Alam, MA Rahman ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Identification of Ideal Trial Sites and Wide Adaptable T. Aus Rice Genotypes Suitable for Bangladesh <p>GGE biplot study is an effective tool for many crops including rice to identify mega-environments, ideal trial site and suitable genotypes for general and specific adaptation. An attempt was made to find promising T. Aus rice genotypes having suitable grain quality, better agronomic parameters and ideal test location for T. Aus rice growing areas. In this study, a total of 11 promising genotypes along with two popular cultivars BR26 and BRRI dhan48 were tested across six locations; Cumilla, Gazipur, Rajshahi, Rangpur in T. Aus 2016 and 2017 whereas Habiganj and Kushtia only in T. Aus 2017 season. From GGE biplot study, BRRI RS, Rajshahi (E8) was the most discriminating and ideal location for evaluating T. Aus rice genotypes in Bangladesh condition while BRRI RS, Cumilla 2016 (E1) showed the least discriminating ability and the least representative location. From two years combination data, BR9011-19-1-2 (G6) recorded the highest average grain yield (5.11±0.68 t ha<sup>-1</sup>) but BR9011-46-2-2 (G2) was the most stable genotype having grain yield (4.97±0.62 t ha<sup>-1</sup>) and other stable genotypes with above average yield were BR9011-67-4-1 (G5), BR9011-34-3-2 (G1), BRRI dhan48 (G13), BR9039-28-3-2 (G9) and BR9039-9-1-3 (G8) indicated that these genotypes adapted to favourable environments. BR26 (G12) was found highly variable and less stable across the test environments. In addition, agronomic trait (plant height, growth duration) and grain quality traits were also considered for suitability and wider adaptation in T. Aus growing areas in Bangladesh. BR9011-46-2-2 (G2) and BR9011-19-1-2 (G6) were the better genotypes in most of the locations but, considering all of the parameters, the newly developed rice breeding line BR9011-67-4-1 (G5) has been identified as suitable genotype to release as a new variety for sustainable T. Aus rice production in Bangladesh.</p> <p>Bangladesh Rice j. 2019, 23(2): 77-85</p> M Khatun, MME Ahmed, MA Syed, F Akter, S Das, ME Haq, SS Dipti ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000 Agroclimatic Indices of Rice (Oryza sativa L.) Influenced by Different Planting Times <p>Meteorological variables have an important role in rice production. Considering this study was carried out in Aman season of 2016 at the research field of Bangladesh Rice Research Institute, Gazipur. The research was conducted in order to investigate the effects of planting time on various agrometeorological indices viz., accumulated growing degree days (GDD), heliothermal units (HTU), photo thermal units (PTU), and heat use efficiency (HUE) the yield of advanced/variety grown under different time of transplanting in the Gazipur climatic zone (AEZ, 28). The research was conducted as a split plot using randomized complete block design (RCBD) with three replications. In this research, three planting times (1st Aug, 16th Aug, and 31<sup>th</sup> Aug) and the Advanced lines/varieties BR (Bio) 9786-BC2-119-1-1, BR (Bio) 9786-BC2-132-1-3 and BRRI dhan49 (check variety) were used in the experiment. The results showed that planting time significantly affected on grain yield. Among the genotypes, BR (Bio) 9786-BC2-119-1-1 and BR (Bio) 9786-BC2-132-1-3 produced significantly higher grain yield than check variety BRRI dhan49 in all the planting times. The variety (BRRI dhan49), transplanted at the first August, took maximum calendar days (136 days) to attain different phenological stages till physiological maturity coupled with highest growing degree days (2420 degree day) for maturity, lowest heat use efficiency (2.25 kg ha<sup>-1</sup> c<sup>-1</sup> day<sup>-1</sup>), highest heliothermal units (15141.01 degree day hours) and lowest photothermal units (29678.81 degree day hours) resulting lower yield. Among the advanced lines the line BR (Bio) 9786-BC2-119-1-1 took higher calendar day (134 days) to attain different phenological stages till physiological maturity coupled with higher growing degree days (2393 degree day) for maturity, heat use efficiency (2.76 kg ha<sup>-1</sup> c<sup>-1</sup> day<sup>-1</sup>), heliothermal units (13759.75 degree day hours) and photothermal units (30112.51 degree day hours) resulting higher yield. Taken together, the results indicate that the advanced line, BR (Bio) 9786-BC2-119-1-1, could be select as a best advanced line to release as a variety due to its higher grain yield.</p> <p>Bangladesh Rice j. 2019, 23(2): 87-94</p> A Sultana, NY Shaikh, MS Zahan, R Akter, SA Islam, M Issak ##submission.copyrightStatement## Fri, 17 Jul 2020 00:00:00 +0000