CYP3A Genotypes in Bangladeshi Tuberculosis Patients

Authors

  • AA Maruf Department of Clinical Pharmacy & Pharmacology, Faculty of Pharmacy, University of Dhaka
  • MU Ahmed Department of Clinical Pharmacy & Pharmacology, Faculty of Pharmacy, University of Dhaka
  • M.A.K. Azad Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka
  • M Ahmed National Institute of Diseases of Chest and Hospital (NIDCH), Dhaka
  • A Hasnat Department of Clinical Pharmacy & Pharmacology, Faculty of Pharmacy, University of Dhaka

DOI:

https://doi.org/10.3329/bmrcb.v38i1.6978

Keywords:

Genetic polymorphisms, CYP3A4, CYP3A5, PCR-RFLP, Bangladeshi TB patients

Abstract

The purpose of this study is to investigate the genotype and allelic frequencies of CYP3A in Bangladeshi Tuberculosis (TB) patients which may help for individualized drug dosing and improved therapeutics. Genotyping was done using the extracted genomic DNA from 90 TB patients followed by amplification of target alleles by PCR. Amplified alleles were then digested by restriction enzymes followed by gel electrophoresis & sequencing to identify the targeted alleles namely CYP3A4*1B, CYP3A4*2, CYP3A4*4, CY3A4*5, CYP3A4*6, CYP3A4*10, CYP3A4*18, and CYP3A5*3. In TB patients, no samples were positive for CYP3A4*2, CYP3A4*4, CYP3A4*5, CYP3A4*6, CYP3A4*10, and CYP3A4*18 alleles. One sample was found to be heterozygous for CYP3A4*1B (1.11%). The wild homozygous (CYP3A5*1/*1) genotype frequency was 7.78%, the heterozygous (CYP3A5*1/*3) frequency was 42.22% and the homozygous mutant (CYP3A5*3/*3) frequency was 50% in Bangladeshi TB patients. The absence of the common polymorphic gene suggests that there will be no impact of CYP3A drug metabolizing enzymes on antituberculosis drugs.

DOI: http://dx.doi.org/10.3329/bmrcb.v38i1.6978

Bangladesh Med Res Counc Bull 2012; 38: 1-5

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Published

2012-04-22

How to Cite

Maruf, A., Ahmed, M., Azad, M., Ahmed, M., & Hasnat, A. (2012). CYP3A Genotypes in Bangladeshi Tuberculosis Patients. Bangladesh Medical Research Council Bulletin, 38(1), 1–5. https://doi.org/10.3329/bmrcb.v38i1.6978

Issue

Section

Research Papers