Screening of Extended Spectrum Beta-Lactamase Producing Bacteria in Clinical Liquid Waste
AbstractAdmixture of Clinical Liquid Waste (CLW) discharging into Ecological Water Bodies (EWB) causes significant pollution with resistant bacteria. The issue is significant in Bangladesh where CLW management is at early stage of development. Extended-Spectrum ?-Lactamase (ESBL) producing bacteria confer resistance to Broad Spectrum ?-Lactam Antibiotics (BSBLA) which has become a global concern now. The aim of this study was to characterise and assess the occurrence of ESBL producing bacteria in CLW of Bangladesh. Three CLW samples were collected from two leading hospitals in Dhaka City, Bangladesh in the year 2012. A total 166 isolates were retrieved and screened for ESBL production by the Double Disk Diffusion Synergy Test (DDST). Isolates with ESBL phenotype were further characterized by antibiotic susceptibility testing, PCR and sequencing of ?-lactamase genes. A total of 30 ESBL producers with Multi Drug Resistant (MDR) properties were isolated. Gene specific PCR against blaTEM, blaCTX-M, and blaSHV detected CTX-M as major ESBL genotype (83%) followed by occurrence of TEM-genotypes (40%). Prevalence of SHV was low in CLW samples (3%). More than 20% of CLW -ESBL isolates possessed both the TEM and CTX-M genotypes. The predominant ESBL isolates were phylogenetically identified as Escherichia spp. Seventy seven percent followed by Citrobacter spp. (17%), Klebsiella spp. (3%), and Yokenella spp. (3%). This investigation demonstrates that CLW possesses ESBL producing MDR bacteria which might act as potential threat to disseminate resistant determinants in the surrounding environment.
300 Online View
Copyright (c) 2017 M Anwar Hossain, Munawar Sultana, Nazratan Naeem, Sabia Sultana, Khandokar Fahmida Sultana, Sanjoy Kumar Mukharje
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms.
Authors retain copyright and grant the journal right of first publication.
Articles in the Bangladesh Medical Research Council Bulletin are licensed under a Creative Commons Attribution 4.0 International License (CC-BY) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).