Enhanced detection rate of typhoid fever among clinically suspected patients in a tertiary referral hospital in Dhaka, Bangladesh using nested polymerase chain reaction technology

Authors

  • Shafinaz Khan Dr. Sirajul Islam Medical College, Mougbazar, Dhaka
  • Ruhul Amin Mia Department of Microbiology, Bangabandhu Sheikh Mujib Medical College, Dhaka
  • Sumaiya Khatun Department of Microbiology, City Medical College, Gazipur.

DOI:

https://doi.org/10.3329/bmrcb.v41i3.29971

Keywords:

Typhoid fever, Nested polymerase chain reaction, Diagnosis

Abstract

A nested polymerase chain reaction (PCR) specific for Salmonella enterica subspecies enteric serovar Typhi was used for the detection of the pathogen in blood. This study was done during the period of March 2013 to February 2014. A total of 80 clinically suspected cases of typhoid fever were included in the study. Blood was collected from all participating individuals. Nested PCR targeting the flagellin gene (fliC) of Salmonella Typhi & blood culture were done for each of the cases. The positivity rate of PCR & blood culture was 70%& 20% respectively. The positivity rate of PCR was significantly higher than blood culture (P< 0.05). With the nested PCR, S. Typhi DNAs were detected from blood specimens of 67.2% (43/64) patients among the suspected typhoid fever cases on the basis of clinical features but with negative cultures. We conclude that the PCR technique could be used as a novel diagnostic method of typhoid fever, particularly in culture-negative cases in an endemic country like Bangladesh.

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Published

2016-10-17

How to Cite

Khan, S., Mia, R. A., & Khatun, S. (2016). Enhanced detection rate of typhoid fever among clinically suspected patients in a tertiary referral hospital in Dhaka, Bangladesh using nested polymerase chain reaction technology. Bangladesh Medical Research Council Bulletin, 41(3), 138–143. https://doi.org/10.3329/bmrcb.v41i3.29971

Issue

Section

Research Papers