A simple biological marker to differentiate the types of Herpes Simplex Viruses in resource-limited settings

Authors

  • T Akter Department of Microbiology, Holy Family Red Crescent Medical College, Dhaka
  • S Tabassum Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka
  • A Nessa Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka
  • M Jahan Department of Virology, Bangabandhu Sheikh Mujib Medical University, Dhaka

DOI:

https://doi.org/10.3329/bmrcb.v38i1.10448

Keywords:

Herpes simplex

Abstract

Herpes simplex viruses (HSV) multiply readily on the chorioallantoic membrane (CAM) of embryonated hens egg and produce easily visible foci or pocks on this membrane. In the present study, pocks produced by the two antigenic types of HSV (1 & 2) were compared to evaluate the effectiveness of typing HSV isolates by pock size on CAMs. A total of 57 HSV isolates from both non-genital and genital samples were typed by the pock size produced on the CAMs of fertile hens eggs. Twenty two HSV isolates yielded visible pocks on CAM, of which 9 (40.9%) produced small pocks, while 13 (59.1%) produced large pocks. All pocks produced on CAM were confirmed by antigenic typing by the Direct Fluorescent Antibody (DFA) method. HSV isolates which produced small pocks were in complete (100%) concordance with HSV type-1, while those producing larger pocks were in full (100%) concordance with HSV type-2. Thus, the pock size on CAM of embryonated fertile hens egg may be used as a simple and relatively inexpensive biological marker for the differentiation of HSV types 1 & 2.

DOI: http://dx.doi.org/10.3329/bmrcb.v38i1.10448

Bangladesh Med Res Counc Bull 2012; 38: 23-26

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Published

2012-04-22

How to Cite

Akter, T., Tabassum, S., Nessa, A., & Jahan, M. (2012). A simple biological marker to differentiate the types of Herpes Simplex Viruses in resource-limited settings. Bangladesh Medical Research Council Bulletin, 38(1), 23–26. https://doi.org/10.3329/bmrcb.v38i1.10448

Issue

Section

Research Papers