Platelet Rich Fibrin for the Treatment of Cleft like Gingival Recession : A Case Report

Background: Gingival recession exposes the tooth’s root with aesthetic disfigurement, frequently brings a patient to a dentist’s office for the objectionable aesthetics caused by the disease. This case report addressed the management of localized cleft like gingival recession in the tooth no 31 with the aid of Platelet Rich Fibrin (PRF). Methods: Mucosal flap was retracted from tooth no 41 to 33 followed by curettage and root planning on the effected tooth (where there was gingival recession). Allogenic bone graft was applied over the affected area, covered with freshly prepared autogenous PRF membrane, flap was repositioned and sutured. Results: Within six month after surgery previously denuded root surface of the treated incisor became entirely covered with mucosa and good aesthetics was regained; which after 4 years, maintained stable gingival contour and aesthetics. Conclusion: The result in the presented case indicate that PRF can be applied when cleft like gingival recession due to malocclusion is necessarily to be treated uneventfully with good aesthetics.


Introduction
Gum recession commonly manifestedas tooth looking longer than normal, occurs when the edge of the gingival margins moves away from the crown of the tooth.The two main predisposing factors are tooth malposition and tooth to alveolar bone relationship. 1If left untreated, the supporting tissue and bone structures of the teeth can be severely damaged, and may ultimately result in tooth loss.Studies have shown that where the shape and position of teeth results in a thin alveolar bone, these areas are predisposed to fenestrations, dehiscence, and gingival recession. 2ther aetiologic factors that have been implicated are tooth brushing trauma, mechanical trauma including biting habits, local irritants including plaque and calculus, iatrogenic restorations, associated high frenal attachments and periodontal disease. 3However, what frequently brings a patient to a dentist's office is not pain, but rather Treatments typically result in aesthetic improvement, elimination of sensitivity and a decreased risk of developing root caries. 4anagement of gingival recession may include several methods such as pocket depth reduction, the gingival grafting, Pinhole Surgical Technique (PST™), Guided Tissue Regeneration (GTR) using collagen membrane and more recently with Platelet-rich plasma (PRP) or Platelet-rich fibrin (PRF) to accelerate soft-tissue and hard-tissue healing. 5,6,7,8se presentation A nine years old boy, came to the Orthodontics Department of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka with the complaint of compromised esthetics at tooth no 31 along with bleeding during tooth brushing on his clinical examination a cleft like gingival recession that measured 6 mm with surrounding red festoon seen on tooth no 31.(figure 1).He had mixed dentition with mild crowding.Guided Tissue Regeneration using PRF membrane along with

Clinical procedures
Preparation of PRF: Fifty ml blood is drawn into five10-ml test tubes without any anticoagulant and centrifuged immediately at 2,700 rpm for 12 min using a tabletop centrifugal mechine. 9The resultant product consists of the three layers and the PRF clot was formed in the middle layer of the test tube (figure 2).PRF was obtained in the form of a membrane by squeezing out the fluids in the fibrin clot. 9rgical procedure:At first the surgical area of the patient's mouth was prepared using chlorhexidine mouthwash.Bilateral incisive nerve block was given to anesthetize the surgical area.A crestal incision was made from tooth no 41 to 33, mucosal flap was retracted and curettage -root planning done in the effected tooth no 31.(figure 3) 0.5cc allogenic bone graft was applied over the affected area, covered with freshly prepared autogenous PRF.Finally flap repositioned and sutured.(figure 4)

Results
Within six month after surgery impressive attachment had developed, previously denuded root surface of the treated incisor became entirely covered.(figure 5) The marginal tissue felt well attached; probing depth found minimal and good esthetics was retained.A follow up period of 4 years, stable gingival contour and aesthetics maintained.(figure 6)

Discussion
PRF described first by Choukrounet al. 10 has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over the traditionally used methods.The free gingival graft, most widely used procedure because of using autogenous graft, is very sensitive.Graft's dimensions and border characteristics impact root coverage success while stabilization and immobilization of the graft also has a crucial role.There is increased discomfort and potential for postoperative bleeding from the donor area by virtue of a large wound that heals by secondary intention. 5Platelet-Rich Plasma (PRP) is a platelet concentrate that is being used widely to accelerate soft-tissue and hard-tissue healing.Sanchez et al. 11 have elaborated on the potential risks associated with the use of PRP.The preparation of PRP involves the isolation of PRP from autologous blood, a double centrifugation technique is required after which gel formation is accelerated using calcium chloride and bovine thrombin.It has been discovered that the use of bovine thrombin may be associated with the development of antibodies to the factors V, and XI, resulting in the risk of life-threatening coagulopathies.Bovine thrombin preparations have also been shown to contain factor V, which could result in the stimulation of the immune system when challenged with a foreign protein.
But PRF eliminates the redundant process of adding anticoagulant as well as the need to neutralize it.Because of the absence of an anticoagulant, blood begins to coagulate as soon as it comes in contact with the glass surface.Here the conversion of fibrinogen into fibrin takes place slowly with small quantities of physiologically available thrombin present in the blood sample itself.Thus, a physiologic architecture that is very favorable to the healing process is obtained due to this slow polymerization process.

Conclusion
The result in the presented case indicates that cleft like gingival recession is necessarily to be treated uneventfully with good aesthetics with the application of the PRF with bone graft.However, source of trauma should be eliminated by proper adjustment of the occlusion for stable result as done in this case.

Figure 1 :
Figure 1: Preoperative figure showing a 6mm cleft like gingival defect on tooth no 31

Figure 2 :Figure 3 :
Figure 2: Prepared three layer including middle layer consisting of PRF after centrifuge

Figure 4 :
Figure 4: Retracted & sutured flap immediately after bone graft and PRF placement

Figure 5 :Figure 6 :
Figure 5: Six months after surgery.Repair of the defect with good esthetics

1
Department of Conservative Dentistry & Endodontics, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh; 2 Department of Orthodontics, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh allogenic bone graft was planned.Selective grindings of some teeth was carried out to