IMMUNOHISTOCHEMICAL STUDY OF THE POSTNATAL DEVELOPMENT OF LYMPHOID TISSUES AND MUCOSA OF BROILERS
The experiment was carried out in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. A total 15 (fifteen) day-old "Cobb-500" chickens (broilers) of both sexes were purchased from "Kazi Farm Ltd." Mymensingh. For immunohistochemical staining purpose, the bursa of Fabricius, cecal tonsils and ileum was collected from postnatal broiler chickens after sacrificing by cervical subluxation method. Sample (n=3) was collected every 7 days interval starting from day 3 up to 32 days of age. Immunohistochemical study revealed that in the bursa of Fabricius, the Igs positive cells (IgA, IgG and IgM) were found principally beneath the capsule, around the follicles and in the cortex and also medulla. In all stages of development (from day 3 to day 32) IgM positive cells were more followed by IgG and IgA positive cells. In the cecal tonsils, Igs positive cells (IgA, IgG and IgM) were distributed within the lymphatic nodules, lamina propria and in the core of the villi. Early in the postnatal development (at day 3), no IgA positive cells were found in the cecal tonsils and IgM positive cells were more than IgG positive cells. At day 32, IgA positive cells were more than IgG and IgM positive cells. With the advancement of age, the Igs positive cells (IgA, IgG and IgM) were increased significantly. In the early postnatal development (at day 3) no immunoglobulin positive cells were found in the ileum. From the later stage (at Day 11), Igs positive cells (IgA, IgG and IgM) were found distributed in the lamina propria, around the intestinal glands and in the core of the villi of ileum. Immunoglobulin positive cells (IgA, IgG and IgM) were increased gradually with increasing age and IgA positive cells were more than IgG and IgM positive cells at day 32.