TY - JOUR AU - Goswami, B AU - Hoque, MI AU - Khan, S AU - Sarker, RH PY - 2020/09/24 Y2 - 2024/03/29 TI - In vitro regeneration of three varieties of Brassica campestris L. grown in Bangladesh JF - Bangladesh Journal of Scientific and Industrial Research JA - Bangladesh J. Sci. Ind. Res. VL - 55 IS - 3 SE - Articles DO - 10.3329/bjsir.v55i3.49391 UR - https://www.banglajol.info/index.php/BJSIR/article/view/49391 SP - 181-188 AB - <p>A reproducible <em>in vitro </em>regeneration system was developed for three varieties of <em>Brassica campestris </em>L. namely Agrani, BINA Sarisha-10 and BINA Sarisha-6 using hypocotyl and cotyledonary leaf with petiole as explants. MS medium supplemented with 2.0 mg/l BAP and 0.2 mg/l IAA was found to be the best for the multiple shoot formation for all the three varieties. Among three varieties, BINA Sarisha-6 showed best response in terms of shoot regeneration as well as number of shoots per explant (9.0) using hypocotyls as explants. In case of Agrani and BINA Sarisha-10 highest number of shoot per explants were found 8.2 and 7.0, respectively. Interestingly <em>in vitro </em>regenerated shoots of BINA Sarisha-6 and BINA Sarisha-10 were produced <em>in vitro </em>flowers on shoot regeneration media. Best root induction in BINA Sarisha-6, Agrani and BINA Sarisha-10 was achieved on MS media supplemented with 0.5 mg/l IBA. After proper hardening, the <em>in vitro </em>regenerated plantlets were successfully transplanted into soil.</p><p><em>Bangladesh J. Sci. Ind. Res.</em><strong>55(3)</strong><strong>,</strong> 181-188, 2020</p> ER -