TY - JOUR AU - Hossain, Mohammad Musharof PY - 2019/12/23 Y2 - 2024/03/28 TI - Morpho-molecular characterization of Ceratobasidium sp.: A mycorrhizal fungi isolated from a rare epiphytic orchid Gastrochilus calceolaris (J. E. Sm.) D. Don JF - Bangladesh Journal of Plant Taxonomy JA - Bangladesh J. Plant Taxon VL - 26 IS - 2 SE - Articles DO - 10.3329/bjpt.v26i2.44584 UR - https://www.banglajol.info/index.php/BJPT/article/view/44584 SP - 249-257 AB - <p>A mycorrhizal fungus, <em>Ceratobasidium</em> sp. GC (NCBI Gene Bank Accession no&nbsp;GQ369961) associated with the roots of an epiphytic orchid <em>Gastrochilus calceolaris</em> was&nbsp;investigated by cultural morphology, microscopic features and molecular analysis of&nbsp;Internal Transcribed Spacer (ITS) regions sequences of nuclear ribosomal DNA. The&nbsp;colony appearance of the fungal endophyte was fluffy growth pattern and the colour of&nbsp;the young colony was milky white on both surfaces that turned in to brown at maturity on&nbsp;the upper surface and deep brown on reverse surface. The microscopic features of the&nbsp;fungus i.e. hyphal diameter, multi-nucleate vegetative cells, right angle branching pattern&nbsp;with slight constriction at branching point and a dolipore septum near the branching point&nbsp;were observed. All the characters corroborated the identity with anamorphic <em>Rhizoctonia&nbsp;</em>like fungi. The ITS regions sequences of nrDNA and phylogenetic analysis based on the&nbsp;neighbor-joining method showed clustered with <em>Rhizoctonia</em> like fungi, and the&nbsp;maximum identity (98.28%) with <em>Ceratobasidium</em> RR and <em>Ceratobasidium</em> FPUB&nbsp;isolated from <em>Rhynchostylis retusa</em> and <em>Dactylorrhiza hetagera</em>, respectively. Thus, the&nbsp;ITS of nrDNA sequences validated the morphological data. This is the first report of&nbsp;orchid mycorrhizal fungi from Bangladesh.</p> ER -