TY - JOUR AU - Al-Hemaid, Fahad M.A. AU - Ali, M. Ajmal AU - Lee, Joongku AU - Gyulai, Gábor AU - Pandey, Arun K. PY - 2014/06/23 Y2 - 2024/03/29 TI - Application of internal transcribed spacer of nuclear ribosomal DNA for identification of Echinops mandavillei Kit Tan JF - Bangladesh Journal of Plant Taxonomy JA - Bangladesh J. Plant Taxon VL - 21 IS - 1 SE - Articles DO - 10.3329/bjpt.v21i1.19256 UR - https://www.banglajol.info/index.php/BJPT/article/view/19256 SP - 33-42 AB - <p>The present study explored the use of internal transcribed spacers (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA (nrDNA) for identification of <em>Echinops mandavillei </em>Kit Tan,<em> </em>an endemic species to Saudi Arabia<em>. </em>The sequence similarity search using Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses of the ITS sequence of <em>E. mandavillei </em>Kit Tan showed high level of sequence similarity (98%) with <em>E. glaberrimus</em> DC. (section <em>Ritropsis</em>)<em>. </em>The novel primary sequence and the secondary structure of ITS2 of <em>E. mandavillei</em> could have a potential use for molecular genotyping.</p><p>DOI: <a href="http://dx.doi.org/10.3329/bjpt.v21i1.19256">http://dx.doi.org/10.3329/bjpt.v21i1.19256</a></p><p>Bangladesh J. Plant Taxon. <strong>21</strong>(1): 33-42, 2014 (June)</p> ER -