TY - JOUR AU - Umamaheswari, Muthuswamy AU - Madeswaran, Arumugam AU - Asokkumar, Kuppusamy AU - Sivashanmugam, Thirumalaisamy AU - Subhadradevi, Varadharajan AU - Jagannath, Puliyath PY - 2011/12/17 Y2 - 2024/03/19 TI - Study of potential xanthine oxidase inhibitors: In silico and in vitro biological activity JF - Bangladesh Journal of Pharmacology JA - Bangladesh J Pharmacol VL - 6 IS - 2 SE - Research Articles DO - 10.3329/bjp.v6i2.9175 UR - https://www.banglajol.info/index.php/BJP/article/view/9175 SP - 117-123 AB - <p>In an attempt to develop potent anti gout agents, coumarin derivatives and polyphenolic compounds were selected for present study. The docking energy of 2-benzyl coumarin was found to be -7.50 kcal/mol which was less than that of the standard allopurinol (-4.47 kcal/mol). All the selected compounds were found to exhibit lower binding energy (-7.50 to -4.68 kcal/mol) than allopurinol. Docking results confirm that selected compounds showed greater inhibition of xanthine oxidase due to their active binding sites. In xanthine oxidase assay, IC<sub>50</sub> value of 2-benzyl coumarin was found to be 26 ± 1.16 µg/mL, whereas that of allopurinol was 24 ± 0.28 µg/mL. All the compounds exhibited IC<sub>50</sub> values ranging between 26 ± 1.16 to 58 ± 0.74 µg/mL.  In enzyme kinetic studies, coumarin derivatives showed competitive and polyphenolic compounds showed non competitive type of enzyme inhibition. It can be concluded that coumarin derivatives could be a remedy for the treatment of gout and related inflammatory disorders.</p> ER -