TY - JOUR AU - Ding, Ruru AU - Zhu, Ziying AU - Teng, Mengting AU - Ma, Lin AU - Hu, Jiaying AU - Hu, Jiangbiao AU - Zhang, Peng PY - 2019/01/07 Y2 - 2024/03/28 TI - A potential estrogen receptor inhibitor compound 34 induces apoptosis via ROS-independent intrinsic apoptosis in MCF-7 cells JF - Bangladesh Journal of Pharmacology JA - Bangladesh J Pharmacol VL - 14 IS - 1 SE - Research Articles DO - 10.3329/bjp.v14i1.38871 UR - https://www.banglajol.info/index.php/BJP/article/view/38871 SP - 1-8 AB - <p>This study aimed to investigate the anti-tumor effects of compound 34 on MCF-7 cells in vitro, and explore its mechanisms. MTT results showed that compound 34 selectively inhibited estrogen receptor-positive cells proliferation. Hoechst 33342 staining showed nuclear pyknosis, nuclear debris associated with apoptotic bodies. JC-1 staining showed the loss of mitochondrial membrane potential. Although compound 34 increased intracellular reactive oxygen species (ROS), compound 34-induced apoptosis was not prevented by pretreatment with ROS scavengers. Western blotting showed apoptosis-related protein like cytochrome c and cleaved PARP protein increased. Furthermore, docking studies exhibited that compound 34 could bind into ERĪ±. In summary, compound 34 selectively inhibited estrogen receptor positive cells proliferation and induced apoptosis in MCF-7 cells via ROS-independent intrinsic apoptosis in MCF-7 cells. It may be a potential targeted drug of estrogen receptor for therapeutic application of breast cancer.</p><p><strong>Video Clip of Methodology:</strong></p><p>Assay of Cell Proliferation: 5 min 5 sec&nbsp; &nbsp;<a href="https://www.youtube.com/watch?v=HF-6f3Tfckk">Click to watch</a>&nbsp;&nbsp;</p> ER -