TY - JOUR AU - Hussain, Musaddique AU - Raza, Shahid Masood AU - Janbaz, Khalid Hussain PY - 2015/03/31 Y2 - 2024/03/28 TI - Pharmacological basis for the folkloric uses of Buxus wallichiana in gastrointestinal, respiratory and vascular disorders JF - Bangladesh Journal of Pharmacology JA - Bangladesh J Pharmacol VL - 10 IS - 2 SE - Research Articles DO - 10.3329/bjp.v10i2.22285 UR - https://www.banglajol.info/index.php/BJP/article/view/22285 SP - 260-266 AB - <p><em>In vitro</em> study was carried out to explore the pharmacological basis of crude extract of <em>Buxus wallichiana </em>for its folkloric uses in gastrointestinal, respiratory and vascular disorders. In jejunum preparations, crude extract (0.03 ± 1.0 mg/mL) caused a transient spasmogenic effect followed by the spasmolytic effect at higher doses (3.0–10 mg/mL). In atropinized jejunum preparation, crude extract inhibited the spontaneous and K<sup>+ </sup>(80 mM)-induced contraction, suggesting that spasmolytic effect is mediated through the Ca<sup>+2</sup>-channel blockade. The Ca<sup>+2</sup>-channel blockade effect was confirmed when pretreatment of tissue with extract produced a dose-dependent shift in Ca<sup>+2 </sup>concentration-response curves to the right, similarly as verapamil. Furthermore, crude extract exhibited non-specific relaxant effect on carbachol- (1 µM) and K<sup>+ </sup>(80 mM)-induced tracheal contractions, suggesting the coexistence of anticholinergic and Ca<sup>+2</sup>-antagonistic properties. Moreover, it relaxed the K<sup>+ </sup>(80 mM)- and phenylephrine (1 µM)-induced contraction in rabbit aorta, suggesting the Ca<sup>+2</sup>-channel blockade. These findings may validate the folkloric uses of <em>B. wallichiana</em> in constipation, bronchitis, asthma and hypertension.</p> ER -