TY - JOUR AU - Ali, Saqib AU - Igoli, John AU - Clements, Carol AU - Semaan, Dima AU - Alamzeb, Muhammad AU - Rashid, Mamoon Ur AU - Shah, Syed Qaisar AU - Ferro, Valerie A. AU - Gray, Alexander I. AU - Khan, Muhammad Rafiullah PY - 2013/08/01 Y2 - 2024/03/28 TI - Antidiabetic and antimicrobial activities of fractions and compounds isolated from Berberis brevissima Jafri and Berberis parkeriana Schneid JF - Bangladesh Journal of Pharmacology JA - Bangladesh J Pharmacol VL - 8 IS - 3 SE - Research Articles DO - 10.3329/bjp.v8i3.13888 UR - https://www.banglajol.info/index.php/BJP/article/view/13888 SP - 336-342 AB - <p>Methanol extracts of the roots of <em>B. brevissima</em> and <em>B. parkeriana</em> were examined for antidiabetic and antimicrobial activities. Six compounds i.e berberine, 8-oxo-berberine, dehydrocheilanthifoline, columbamine, jatrorrhizine and gluta-mic acid were isolated from the active fractions of these species for the first time. The antidiabetic activity was determined against Protein Tyrosine Phosphatase 1B (PTP 1B), a negative insulin regulator while the antimicrobial activity was performed against four bacterial strains: <em>Mycobacterium marinum</em> ATCC BAA535, sporadic Methicillin Resistant <em>Staphylococcus aureus</em> (SMRSA), endemic Methicillin Resistant <em>Staphylococcus aureus</em> (EMRSA) and <em>Escherichia coli</em> ATCC.8739 at different concentrations.  Amongst the isolated compounds 8-oxo-berberine was found to be the most active antidiabetic (29 % of the positive control) while jatrorrhizine was the most active and selective antimicrobial against SMRSA and EMRSA (-1.2% of the DMSO (D) control).</p> ER -