TY - JOUR AU - Lakshmi, V. AU - Saxena, A. AU - Mishra, S. K. AU - Mishra, M. AU - Srivastava, S. AU - Ghoshal, S. PY - 2008/09/22 Y2 - 2024/03/29 TI - Antiamoebic activity of marine sponge Haliclona exigua (Krikpatrick) JF - Bangladesh Journal of Pharmacology JA - Bangladesh J Pharmacol VL - 4 IS - 1 SE - Research Articles DO - 10.3329/bjp.v4i1.1083 UR - https://www.banglajol.info/index.php/BJP/article/view/1083 SP - 55-59 AB - <p>The methanol and methanol-chloroform (1:1) extracts of the freshly collected Haliclona exigua showed minumim inhibitory concentration (MIC) of 125 ?g/mL and 250 ?g/mL respectively in in vitro studies, but when both of these were tested in vivo in rats, only methanol-chloroform showed 80% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entameba histolytica. Therefore only methanol-chloroform extract was further fractionated into four fractions (n-hexane, chloroform, n-butanol soluble and n-butanol insoluble fractions). Out of these, only n-hexane and n-butanol soluble fractions showed 80% inhibition of trophozoites at 900 mg/kg dose. Further the chromatography of the n-butanol fraction yielded araguspongin-C which showed promising results at different doses.</p> ER -