@article{Bajpai_Singh_Mehta_2016, title={Chemical characterization and mode of action of Ligustrum lucidum flower essential oil against food-borne pathogenic bacteria}, volume={11}, url={https://www.banglajol.info/index.php/BJP/article/view/25487}, DOI={10.3329/bjp.v11i1.25487}, abstractNote={<p class="Abstract">Current research analyzes the chemical composition of <em>Ligustrum lucidum</em> flower essential oil obtained by the hydrodistillation, and examines its anti-microbial mode of action against food-borne pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the oil resulted in the determination of 44 different compounds, representing 85.2% of the total oil. The oil (1 mg/disc) showed significant antibacterial effect as diameters of inhibition zones (14.6 ± 0.2 – 19.7 ± 0.3 mm), as well as minimum inhibitory and minimum bactericidal concentrations values (250–1000 and 250–2000 µg/mL), respectively. Based on the susceptibility, <em>L. lucidum</em> flower oil revealed its mode of action on membrane integrity as confirmed by increased release of extracellular ATP (2.5 and 2.2 pg/mL), leakage of potassium ions (950 and 900 mM/L), loss of 260-nm absorbing materials (4.2 and 3.9 optical density), and increase in relative electrical conductivity (10.6 and 9.8%) against <em>Staphylococcus aureus</em> KCTC-1621 (Gram-positive) and <em>Salmonella enterica</em> ATCC-4731 (Gram-negative), respectively.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">MIC determination: 10 min 32 sec   <a href="https://youtube.com/v/7G3Yr8O_6kY">Full Screen</a>   <a href="https://youtube.com/watch?v=7G3Yr8O_6kY/">Alternate</a></p>}, number={1}, journal={Bangladesh Journal of Pharmacology}, author={Bajpai, Vivek K. and Singh, Siddhartha and Mehta, Archana}, year={2016}, month={Jan.}, pages={269–280} }