Cardioprotective activity of fruit of Garcinia pedunculata on isoprenaline-induced myocardial infarction in rat

The present study reveals the protective effect of Garcinia pedunculata aqueous fruit extract in isoprenaline-induced myocardial infarction in Wistar rats. Isoprenaline (200 mg/kg) administration subcutaneous once daily for two consecutive days significantly (p<0.01) increased the CK-MB, AST, ALT, ALP activity and CRP (p<0.05) levels. Pretreatment with extract (400 mg/kg) orally for 14 consecutive days significantly ameliorated the effect of isoprenaline by reducing the activity of CK-MB and the levels of ALP, SGPT respectively. No significant changes were seen in the case of SGOT activity and CRP levels. Severe necrotic lesions in the myocardial tissue were observed in the isoprenaline-treated group. In extract-treated group, mild degenerative changes of myocardial tissues to nearly normal cytoarchitecture were seen. The results indicate the cardioprotective effect of G. pedunculata against isoprenalineinduced myocardial infarction rat model. Article Info Received: 9 September 2015 Accepted: 1 January 2016 Available Online: 25 January 2016 DOI: 10.3329/bjp.v11i1.24941 Cite this article: Mundugaru R, Udaykumar P, Senthilkumar S, Sudhakara. Cardioprotective activity of fruits of Garcinia pedunculata on isoprenaline induced myocardial infarction in albino rats. Bangladesh J Pharmacol. 2016; 11: 231-35. Cardioprotective activity of fruit of Garcinia pedunculata on isoprenaline-induced myocardial infarction in rat Ravi Mundugaru1, Padmaja Udaykumar2, Sivanesan Senthilkumar3 and Sudhakara Bhat1 Department of Pharmacology and Toxicology, SDM Centre for Research in Ayurveda and Allied Sciences, Udupi 574 118, India; Department of Pharmacology Father Muller Medical College, Mangalore 575 002, India; Department of Research and Development, Saveetha University, Thandalam, Chennai 602 105, India. This work is licensed under a Creative Commons Attribution 4.0 International License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor use as a cardiotonic activity, the present study has been carried out to elucidate cardioprotective activity of aqueous extract of G. pedunculata in isoprenalineinduced myocardial infarction model. Materials and Methods Plant material and extract preparation Fruits of G. pedunculata were collected from Assam region, India with the help of a botanist during March of 2012. It was authenticated in Pharmacognosy Laboratory, SDM Centre for Research in Ayurveda and Allied Sciences, Udupi. The voucher specimen (No. 13100501) has been deposited for further future reference. The powder of fruits of G. pedunculata was prepared in the Pharmacy attached to SDM College Ayurveda, Udupi, from authenticated plant material. The powder obtained from a single batch was used throughout the experimental study. Two fifty grams of the fruit powder of G. pedunculata was soaked in 2L of distilled water for 24 hours; it was filtered and concentrated in a water bath. The concentrated extract of G. pedunculata was used for the isoprenaline-induced cardioprotective activity. Experimental animals Male Wistar albino rats weighing 180-220 g were obtained from animal house attached to SDM Centre for Research in Ayurveda and Allied Sciences, Udupi. They were fed with standard diet and water ad libitum. The animals were housed in a controlled environment with 12:12 hours light and dark cycle, temperature of 25°C and relative humidity 50%. The experimental animals were acclimatized for two weeks in the laboratory condition before the experiment. The experiments were carried out in accordance with the guidelines of institutional ethical committee under the reference number SDMCRA/IAEC/2012-13DGM 03ab.


Introduction
Myocardial infarction is the acute ischemic condition where profound necrosis of myocardial tissue occurs.This mainly results of imbalance between coronary blood supply and myocardial oxygen demand (de Bono et al., 1992).Isoprenaline is a synthetic catecholamine acts as a β adrenergic agonist.It has been reported to produce myocardial infarction in higher dose (Jordan et al., 1999;Panda and Naik, 2009).Isoprenaline undergoes auto-oxidation and generate highly cytotoxic free radicals.These free radicals stimulate per-oxidation of membrane phospholipids causing severe damage to myocardial membrane.Subsequently, the inflammation event takes place with infiltration of neutrophils into the infarcted area where it can promote myocardial cell damage through the release of proteolytic enzymes.It intern produces cytotoxic reactive oxygen species (Kannan and Quine, 2013).Thus, the model is used as a novel method to produce myocardial infarction in experimental animals.Amlavetasa Garcinia pedunculata is a medicinal herb that belongs to the family Clusiaceae.Tribal people of Assam region were widely using the dried fruits of the plant as cardiotonic along with their diet (Comalada et al., 2005).It has been reported that the dried fruits of GP are used for asthma, cough, bronchitis and fever (Jayaprakasha et al., 2006).The phytochemical analysis has shown the dried fruit rinds and pericarp contains (-) hydroxy citric acid, benzophenones, pedenculol, garcinol and cambogin (Kagyung et al., 2010).It has been screened for its anti-inflammatory, in vitro anti-oxidant and hepatoprotective activity (Patiri and Borah, 2007;Ravi Mundugaru et al., 2014).At present, there is an increased attention on phytochemicals and polyphenols such as alkaloids, flavonoids, xanthenes derived from different plant species.These chemicals possess excellent anti-oxidant properties and have potential therapeutic values in prevention and management of cardiovascular disease (Sahu et al., 1989).use as a cardiotonic activity, the present study has been carried out to elucidate cardioprotective activity of aqueous extract of G. pedunculata in isoprenalineinduced myocardial infarction model.

Plant material and extract preparation
Fruits of G. pedunculata were collected from Assam region, India with the help of a botanist during March of 2012.It was authenticated in Pharmacognosy Laboratory, SDM Centre for Research in Ayurveda and Allied Sciences, Udupi.The voucher specimen (No. 13100501) has been deposited for further future reference.The powder of fruits of G. pedunculata was prepared in the Pharmacy attached to SDM College Ayurveda, Udupi, from authenticated plant material.The powder obtained from a single batch was used throughout the experimental study.Two fifty grams of the fruit powder of G. pedunculata was soaked in 2L of distilled water for 24 hours; it was filtered and concentrated in a water bath.The concentrated extract of G. pedunculata was used for the isoprenaline-induced cardioprotective activity.

Experimental animals
Male Wistar albino rats weighing 180-220 g were obtained from animal house attached to SDM Centre for Research in Ayurveda and Allied Sciences, Udupi.They were fed with standard diet and water ad libitum.The animals were housed in a controlled environment with 12:12 hours light and dark cycle, temperature of 25°C and relative humidity 50%.The experimental animals were acclimatized for two weeks in the laboratory condition before the experiment.The experiments were carried out in accordance with the guidelines of institutional ethical committee under the reference number SDMCRA/IAEC/2012-13DGM 03ab.

Chemicals
Isoprenaline was purchased from Sigma Chemicals USA (product code-101128695).

Acute oral toxicity test
The acute oral toxicity study was carried out as per OECD guidelines 425 using AOT software.The aqueous extract of fruits of G. pedunculata was made into a suspension in 0.5% gum acacia and dosed in the following order 175, 550 and 2000 mg/kg body weight.After the dosing, the animals were observed for 14 days for mortality.The LD50 was determined by using AOT software.

Induction of myocardial infarction
Isoprenaline was dissolved in normal saline and injected subcutaneously at a dose of 200 mg/kg body weight once daily for 2-consecutive days (Priscilla and Prince, 2009;Kumaran and Prince, 2010).

Experimental design
Rats were randomly divided into three groups of six rats each.Group I received 0.5% gum acacia for 14 consecutive days and served as normal control group.Group II rats received 0.5% gum acacia for 14 consecutive days and in addition received isoprenaline 200 mg/ kg, subcutaneously at an interval of 24 hours.Group III rats were pre-treated with 400 mg/kg of extract for a period of 14 consecutive days and in addition received isoprenaline 200 mg/kg, subcutaneously on 14 th and 15 th day at an interval of 24 hours.
Twenty-four hours after the last dose of isoprenaline, animals were anesthetized and blood samples were collected from retro-orbital puncture for estimation of biochemical parameters such as CK-MB in serum was that was determined using CK-MB diagnostic kit and CRP was measured using a specific immunoassay kit (Immunospec Corporation, USA).The serum biochemical markers such as AST, ALT and ALP measured according to standard methods (Bradly et al., 1972;Wilkinson et al., 1969).Rats were then sacrificed under deep anesthesia and the heart was rapidly isolated washed with ice-cold saline.The tissue was then transferred to 10% formalin for histological examination.The tissue was embedded in paraffin.The section was cut at 5 µm thickness and stained with hematoxyline-eosin stain and mounted in diphenyl xyline.The histopathological changes of heart tissue were observed under a compound microscope and their micro-photographs were taken (Bancroft and Gamble, 2002).

Statistical analysis
The experimental data were expressed as mean ± SEM.Statistical analysis was carried out by one-way analysis of variance followed by Dennett multiple t-test.A level for p˂0.05 was considered to be statistically significant.

Results
In acute oral toxicity study in any dose up to 2,000 mg/ kg of aqueous extract of fruits of the plant G. pedunculata did not produce any mortality.This indicates that LD50 is much more than 2,000 mg/kg and hence 1/5 th of the dose was selected for the present cardioprotective study.
A significant elevation in the serum CK-MB, SGOT, SGPT, ALP activity and C-reactive protein levels were observed in isoprenaline-treated myocardial infarcted rats as compared to Group I control rats (Table I).The extract pre-treated group significantly reduced the activity of serum biomarkers such as CK-MB, SGPT and ALP and caused mild to moderated reduction of SGOT activity and CRP levels in serum (Table I).

Histological examination of heart tissue
Microscopic examination of the section of heart from different groups was carried out to assess the impact of isoprenaline injection on cardiac cytoarchitecture and its modulation by test drug administration.
Sections of heart from normal control rats exhibited normal cytoarchitecture.The myofibrillar structure was normal with striations, branching and continuity with adjacent myofibrils.
Examination of sections of heart from isoprenaline group exhibited remarkable changes in the cytoarchitecture.In most of the rats moderate to severe necrotic lesions were found especially in the apical region.The basal region was not affected to great extent.In the area surrounding this region myocarditis, interstitial edema and focal cell infiltration were observed.Loss of myocardial fibres was observed in some areas.Hemorrhage and fatty changes were observed in some sections.Severe necrotic lesions were observed in all the rats in this group (Figure 1).
In G. pedunculata extract-treated isoprenaline injected group, the severity of the degenerative changes was found to be moderate and less in comparison to the control isoprenaline group.Moderate necrosis with cell infiltration was observed in sections from two rats.Mild to moderate necrosis and degenerative changes in the form myocarditis and fatty changes were observed in sections from the remaining rats.

Discussion
In the present study, there is a significant elevation in serum CK-MB, ALT, AST and ALP levels in isoprenaline treated group.Pre-treatment with G. pedunculata extract for 14 days showed a marked decrease in the elevated serum biomarkers.This suggests the test drug has the potential to inhibit free radical generation and subsequent cell damage.The earlier report suggests that the test drug has strong anti-oxidant potential.This might be the possible mechanism of the protective outcome of the present study.
Subsequently after myocardial infarction caused by isoprenaline inflammatory process involved in the myocardial tissue damage and cellular necrosis.It is reported that the infarcted area undergoes local necrosis and myocyte apoptosis resulting in complementary activation, free radical generation and accumulation of cellular debris.This is followed by infiltration of neutrophils and macrophages into the infarcted area, where they can promote myocardial cell damage by release of proteolytic enzymes and cytokines.Pretreatment with extract showed a marked decrease in the serum levels of CRP.It is also reported that extract has good anti-inflammatory activity (Ravi Mundugaru et al., 2014).
Histological examination of sections of heart from isoprenaline treated group exhibited remarkable changes in the cytoarchitecture.In most of the rats moderate to severe necrotic lesions were found especially in the apical region than basal.In the area surrounding this region myocarditis interstitial edema, focal cell infiltration, hemorrhages and fatty changes were observed.Extract-treated group showed maximum protective effect by reduced histological changes as compared to isoprenaline-treated myocardial infarcted rats.

Conclusion
Aqueous extract of fruits of G. pedunculata protected experimentally induced myocardial infarction in rats as revealed by the amelioration of histological changes and biochemical and inflammatory markers of cardiac tissue damage.J Am Coll Cardiol. 2002;39: 1917-23.

Figure 1 (
Figure 1 (A, B): Photomicrographs of representative heart sections from normal control group treated with gum acacia shows normal cytoarchitecture.Myocardium (MC); (C, D): Photomicrographs of representative heart sections from isoprenaline group shows moderate to severe necrotic lesions (NC), myocarditis interstitial edema (Oed), focal cell infiltration (Fc), hemorrhages (Hg) and fatty changes were observed; (E, F): Photomicrographs of representative heart sections from test drug + Isoprenaline injected rats shows mild moderate necrotic lesions (NEC) and myocardial interstitial edema (Oed).The test drug showed a maximum protective effect by reduced histological changes

Table I Effects of fruit extract of G. pedunculata on biochemical parameters
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