Anti-diabetic activity of crude Pistacia lentiscus in alloxan-induced diabetes in rats

The purpose of this study was to investigate the anti-diabetic effect of crude Pistacia lentiscus gum (mastic gum) in alloxan-treated diabetic rat model. The crude P. lentiscus (100 mg/kg) showed significant (p<0.001) reduction in blood glucose as compared to control. Liver function test also showed significant changes (p<0.001) as compared to alloxan-treated group. The results of this study showed that crude P. lentiscus gum have considerable efficacy in curing diabetes and have hepatoprotective effect. Article Info Received: 4 May 2015 Accepted: 17 May 2015 Available Online: 1 July 2015 DOI: 10.3329/bjp.v10i3.23225 Cite this article: Rehman MSU, Kamran SH, Ahmad M, Akhtar U. Antidiabetic activity of crude Pistacia lentiscus in alloxaninduced diabetes in rats. Bangladesh J Pharmacol. 2015; 10: 543-47. This work is licensed under a Creative Commons Attribution 3.0 License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor. Anti-diabetic activity of crude Pistacia lentiscus in alloxan-induced diabetes in rats Muhammad Saad Ur Rehman, Sairah Hafeez Kamran, Mobasher Ahmad and Usman Akhtar University College of Pharmacy, University of the Punjab, Lahore, Pakistan. was used as a suspending agent. The measured amount of mastic gum powder was triturated with water in pestle and mortar, then a few drops of tween 80 were added until uniform paste like consistency achieved. Then water was added with continuous trituration until final suspension formed. The suspension was given to the animals orally by metal dozer. Animals: The research conforms to the Guide for the care and Use of Laboratory Animals by the US National institutes of Health (NIH publication No. 85-23, revised 1985). All animals were treated according to the procedures approved by the Institutional Ethical Committee at the University College of Pharmacy, University of the Punjab, Lahore, Pakistan. Male albino rats weighing 180 -210 g were used. They were acclimatized to animal house conditions. The rats were fed standard rat chow and had free access to water and food until treatment. Experimental design: Diabetes was induced by intraperitoneal administration of alloxan monohydrate (150 mg/ kg) (Rao and Rao, 2001). After 3 days, animals with 200400 mg/dL blood glucose or higher were selected and used in experiment. For acute study, rats were divided into four groups of five rats each; group I (normal rats); group II (diabetic untreated rats); group III (diabetic treated with standard drug glibenclamide); group IV (diabetic treated with crude Pistacia gum 100 mg/kg). Blood glucose was estimated at 6 hours and 24 hours after the dose. For chronic study, rats were divided into four groups of five rats each; Group I (normal rats); Group II (diabetic untreated rats); Group III (diabetic treated with glibenclamide); Group IV (diabetic treated with crude Pistacia gum 100 mg/kg). Blood glucose and serum ALT, AST were estimated at regular intervals, ie., 7th, 14th and 21st day during chronic treatment with extract and glibenclamide. Blood biochemistry: Blood samples were taken from all experimental groups. Serum was isolated from blood of all animal groups at different hours in acute study and at different days in chronic study. The amount of serum Blood glucose (GOD/POD method) (Sushruta et al., 2007) AST, ALT was estimated using commercial kits according to the manufacturer’s protocol. Statistical analysis: Different treatment group’s vs control groups was performed by one-way ANOVA with Bonferroni post-hoc test using Graphpad Prism. Pvalue of less than 0.05 was considered statistically significant. Phytochemical test of P. lentiscus Test for alkaloids: Draggondroff’s reagent was used for the detection of alkaloids in the methanolic extract of the plant. The methanolic extract of the plant was spotted on the TLC plate. The TLC plate was sprayed with Draggondroff’s reagent. Bright yellow spots appeared on the TLC plate which indicated the presence of alkaloid in the methanolic extract. Test for flavonoids: The methanolic extract of the plant was spotted on the TLC plate for the detection of flavonoids. The TLC plate was then sprayed with aluminum chloride. Bright yellow florescence appeared on long wavelength which indicated the presence of flavonoids in the methanolic extract. For the confirmation of presence of flavonoids in the P. lentiscus, the methanolic extract of the plant was spotted on the TLC plate. The TLC plate was then sprayed with antimony chloride. The plate was then observed in long UV wavelength. Bright fluorescence appeared which confirmed the presence of flavonoids. Test for steroids, phenols and sugars: Methanolic extract of P. lentiscus was spotted on TLC plate, the plate was then sprayed with anisaldehyde. Red colored spots appeared which indicated the presence of steroids, sugars and phenols in the methanolic extract of the plant. Methanolic extract of P. lentiscus was spotted on the TLC plate, concentrated sulphuric acid was applied to the plate. Brown color appeared confirming the presence of sugars in the methanolic extract. Test for lipids: For the detection of lipids in the methanolic extract of P. lentiscus, the extract was spotted on TLC plate, the plate was then sprayed with 2, 7-dichlorofluorescein. Fluorescence appeared on the plate indicating the presence of lipids. Test for terpenes: TLC plate was spotted with methanolic extract of the plant. Ceric sulphate was then sprayed on the TLC plate. The spot turned dark brown which indicated the presence of terpenes or terpenoids in the plant. Test for saponins: Methanolic extract of plant was mixed with equal quantity of water in a test tube. Test tube was shaken vigorously. No foam appeared on vigorous shaking. This indicated the absence of saponins in the plant extract.

was used as a suspending agent. The measured amount of mastic gum powder was triturated with water in pestle and mortar, then a few drops of tween 80 were added until uniform paste like consistency achieved. Then water was added with continuous trituration until final suspension formed. The suspension was given to the animals orally by metal dozer.

Animals:
The research conforms to the Guide for the care and Use of Laboratory Animals by the US National institutes of Health (NIH publication No. 85-23, revised 1985). All animals were treated according to the procedures approved by the Institutional Ethical Committee at the University College of Pharmacy, University of the Punjab, Lahore, Pakistan. Male albino rats weighing 180 -210 g were used. They were acclimatized to animal house conditions. The rats were fed standard rat chow and had free access to water and food until treatment.
Experimental design: Diabetes was induced by intraperitoneal administration of alloxan monohydrate (150 mg/ kg) (Rao and Rao, 2001). After 3 days, animals with 200-400 mg/dL blood glucose or higher were selected and used in experiment. For acute study, rats were divided into four groups of five rats each; group I (normal rats); group II (diabetic untreated rats); group III (diabetic treated with standard drug glibenclamide); group IV (diabetic treated with crude Pistacia gum 100 mg/kg). Blood glucose was estimated at 6 hours and 24 hours after the dose. For chronic study, rats were divided into four groups of five rats each; Group I (normal rats); Group II (diabetic untreated rats); Group III (diabetic treated with glibenclamide); Group IV (diabetic treated with crude Pistacia gum 100 mg/kg). Blood glucose and serum ALT, AST were estimated at regular intervals, ie., 7th, 14th and 21st day during chronic treatment with extract and glibenclamide.
Blood biochemistry: Blood samples were taken from all experimental groups. Serum was isolated from blood of all animal groups at different hours in acute study and at different days in chronic study. The amount of serum Blood glucose (GOD/POD method) (Sushruta et al., 2007) AST, ALT was estimated using commercial kits according to the manufacturer's protocol.
Statistical analysis: Different treatment group's vs control groups was performed by one-way ANOVA with Bonferroni post-hoc test using Graphpad Prism. Pvalue of less than 0.05 was considered statistically significant.
Phytochemical test of P. lentiscus Test for alkaloids: Draggondroff's reagent was used for the detection of alkaloids in the methanolic extract of the plant. The methanolic extract of the plant was spotted on the TLC plate. The TLC plate was sprayed with Draggondroff's reagent. Bright yellow spots appeared on the TLC plate which indicated the presence of alkaloid in the methanolic extract.
Test for flavonoids: The methanolic extract of the plant was spotted on the TLC plate for the detection of flavonoids. The TLC plate was then sprayed with aluminum chloride. Bright yellow florescence appeared on long wavelength which indicated the presence of flavonoids in the methanolic extract.
For the confirmation of presence of flavonoids in the P. lentiscus, the methanolic extract of the plant was spotted on the TLC plate. The TLC plate was then sprayed with antimony chloride. The plate was then observed in long UV wavelength. Bright fluorescence appeared which confirmed the presence of flavonoids.
Test for steroids, phenols and sugars: Methanolic extract of P. lentiscus was spotted on TLC plate, the plate was then sprayed with anisaldehyde. Red colored spots appeared which indicated the presence of steroids, sugars and phenols in the methanolic extract of the plant.
Methanolic extract of P. lentiscus was spotted on the TLC plate, concentrated sulphuric acid was applied to the plate. Brown color appeared confirming the presence of sugars in the methanolic extract.
Test for lipids: For the detection of lipids in the methanolic extract of P. lentiscus, the extract was spotted on TLC plate, the plate was then sprayed with 2, 7-dichlorofluorescein. Fluorescence appeared on the plate indicating the presence of lipids.
Test for terpenes: TLC plate was spotted with methanolic extract of the plant. Ceric sulphate was then sprayed on the TLC plate. The spot turned dark brown which indicated the presence of terpenes or terpenoids in the plant.
Test for saponins: Methanolic extract of plant was mixed with equal quantity of water in a test tube. Test tube was shaken vigorously. No foam appeared on vigorous shaking. This indicated the absence of saponins in the plant extract.

Results
The effect of treatment with glibenclamide and crude P. lentiscus in diabetic rats were compared and shown in Figure 1 and 2. Blood glucose levels in diabetic rats were significantly higher than treated group at 0,6,24 hour interval (p<0.001). After 6 hour, there was decreased in blood glucose (280.8 ± 9.0) but after 24 hour crude Pistacia gum showed significant decrease (195.2 ± 20.4) as compared to diabetic untreated (352.4 ± 23.6) rats as shown in Figure 1.
To further evaluate the role of crude P. lentiscus on liver after continuous administration of 21 days, serum level of AST and ALT were analyzed in all groups as shown in Figure 3. Serum level of ALT was significantly lowered in crude P. lentiscus treated group at 21 st day (12.8 ± 0.8) as compared to diabetic untreated (34.0 ± 1.8) and glibenclamide-treated groups (22.1 ± 1.9). In addition, serum ALT level was also significantly lowered in crude P. lentiscus-treated group at 21 st day (6.6 ± 0.7) as compared to diabetic untreated (41.0 ± 2.2) and glibenclamide-treated groups (14.9 ± 1.1). Figure 1: Acute glucose activity in serum of all animal groups; ***p<0.001 for diabetic plus crude Pistacia gum 24 hours vs diabetic untreated 24 hours; ***p<0.001 for diabetic plus glibenclamide 24 hours vs diabetic untreated 24 hours Figure 2: Chronic glucose activity in serum of all animals groups; ***p<0.001 for diabetic plus crude Pistacia gum; diabetic plus glibenclamide 7 days vs diabetic untreated 7 days; ***p<0.001 for diabetic plus crude Pistacia gum; diabetic plus glibenclamide 14 days vs diabetic untreated 14 days; a p>0.05 diabetic plus glibenclamide 14 days vs normal 14 days; ## p<0.01 for diabetic plus crude Pistacia gum 14 days vs normal 14 days; ***p<0.001 for diabetic plus crude Pistacia gum; diabetic plus glibenclamide 21 days vs diabetic untreated 21 days; a p>0.05 diabetic plus glibenclamide; diabetic plus crude Pistacia gum 21 days vs normal 21 days

Discussion
In current study, we have investigated the anti-diabetic potential in alloxan-induced diabetes. Alloxan induced diabetes produces selective cytotoxicity in β-cells of pancreas through the generation of free radicals resulting in reduced synthesis and release of insulin (Sakurai et al., 2001). In acute study, mastic gum showed significant decrease in blood glucose that was comparable to glibenclamide by rapid normalization of blood glucose levels while high glucose level was observed in alloxan-treated groups throughout the total time period of study. It is possible that mastic gum and glibenclamide bring about release of insulin from the βcells of pancreas, resulting in normalization of blood glucose. Then we study the chronic administration of mastic gum for 21 days for its anti diabetic effect. It showed highly significant decrease in blood glucose as shown in Figure 2 at different day's interval.
The increase in the plasma concentration of AST, ALT indicated that in diabetic patient there is induced hepatic dysfunction. Liver is necrotized in diabetic patients (Larcan et al., 1979). The concentration of these enzymes increased in plasma mainly due to the leakage from the cytosol of liver into blood stream (Navarro et al., 1993) which is a good indicator of hepatotxic effect of alloxan. In current study, treatment of diabetic rats with mastic gum caused reduction in the concentration of these enzymes in plasma at different days interval compared to the mean values of diabetic untreated rats.
The reduction in AST, ALT is due to outflow of enzymes into the blood as a consequence of alloxan toxicity which causes liver damage. However, crude Pistacia gum treatment groups for 21 days could restore the activities of the above enzymes to their normal levels. This Study shows that crude P. lentiscus gum acts as anti diabetic and heptoprotective effect.