TY - JOUR AU - Aan, Goon Jo AU - Karim, Noralisa Abdul AU - Zainudin, Mohd Shahril Aszrin AU - Makpol, Suzana PY - 2019/08/30 Y2 - 2024/03/29 TI - Tocotrienol-Rich Fraction Modulates Genes Expression in Oxidative Stress-induced Caenorhabditis e legans JF - Bangladesh Journal of Medical Science JA - Bangladesh J Med Sci VL - 18 IS - 4 SE - Original Articles DO - 10.3329/bjms.v18i4.42874 UR - https://www.banglajol.info/index.php/BJMS/article/view/42874 SP - 711-721 AB - <p><strong><em>Objective</em></strong>: The study was done to determine the effect of Tocotrienol rich fraction (TRF) on the expression of RNAs in <em>C. elegans </em>under oxidative stress.</p><p><strong><em>Methods: </em></strong>The nematodes were divided into 4 groups and treated accordingly: control; TRF; hydrogen peroxide (H202); TRF treatment before and after H202-induction (TRF+H202+TRF). Expressions of RNAs were analyzed with Affymetrix Genechip <em>C. elegans </em>Genome Array and Genespring GX11 software where differentially expressed genes were further analyzed using gene ontology (GO). Selected genes (unc-15, cit-1.2, ftn-1, rsks-1, unc-4 and daf-12) were analyzed with RT-qPCR to validate the results.</p><p><strong><em>Results: </em></strong>TRF modulated the expression of 314 genes involved in determination of adult lifespan, regulation of growth and lipid modification. A total of 440 genes involved in RNA metabolic processes, transcription, growth and differentiation of muscle and nerve cells were differently expressed following H202 induction. TRF treatment before and after H202- induction resulted in 438 differentially expressed genes involved in RNA metabolic processes, transcription, response to xenobiotic stimulus and protein amino acid phosphorylation.</p><p><strong><em>Conclusion</em></strong>: TRF modulates the expression of genes involved in the regulation of lifespan in <em>C. elegans</em>.</p><p>Bangladesh Journal of Medical Science Vol.18(4) 2019 p.711-721</p> ER -