@article{Talib_Kashif_Tahir_Ahmed_Khaliq_Mubarik_Abbas_Jahan_Afzal_2019, title={Association of interleukin-6 polymorphism (-634C/G) in the promoter region with diabetic nephropathy in type 2 diabetes mellitus}, volume={18}, url={https://www.banglajol.info/index.php/BJMS/article/view/42878}, DOI={10.3329/bjms.v18i4.42878}, abstractNote={<p><strong><em>Background</em></strong><strong>:</strong>Long term microvascular complications of DM includes: nephropathy, retinopathy, and neuropathy. Increased level of IL-6 and its polymorphism have been associated with diabetic nephropathy.The present study was designed to determine serum level of IL-6 and C/G polymorphism at − 634 position in the promoter region of IL-6 gene in patients with type 2 diabetes mellitus (T2DM) with and without nephropathy.</p> <p><strong><em>Method: </em></strong>This comparative study was comprised of 164 subjects, divided into two groups of 82 subjects in each. The subjects who had diabetes for more than 5 years without nephropathy were labeled as group-I and the subjects who had diabetes for more than 5 years with nephropathy were labeled as group-II. IL-6 polymorphism was detected by RFLP and its level was determined by ELISA technique. Data was analyzed using SPSS 20.0.</p> <p><strong><em>Results: </em></strong>In group-I, 14 (17.1%) patients had CC, 47 (57.3%) had CG and 21 (25.6%) had GG polymorphism. In group-II, 9 (11%) patients had CC, 69 (84.1%) had CG and 4 (4.9%) had GG polymorphism. Pearson’s correlation test determined significant association of IL-6 polymorphism (CC,CG,GG) in both the groups (p <strong><</strong>0.001). In patients of CC polymorphism, median levels of IL-6 was high in group-I (4.2) as compared to group-II (3.2),whereas in patients of CG polymorphism, median levels of IL-6 was high in group-II (3.8) as compared to group I (3.7) while in GG polymorphism, median levels of IL-6 was same in both the groups. On comparison of IL-6 polymorphism and level of IL-6 there was no statistically significant difference between the two groups (p1=0.304, p2=0.425 respectively).</p> <p><strong><em>Conclusions: </em></strong>Statistically significant difference in the frequencies of allele (CC,CG,GG) between two groups is pointing towards the protective role of GG allele as it was predominant in T2DM patients without nephropathy.</p> <p>Bangladesh Journal of Medical Science Vol.18(4) 2019 p.741-747</p>}, number={4}, journal={Bangladesh Journal of Medical Science}, author={Talib, Rakshanda and Kashif, Muhammad and Tahir, Romeeza and Ahmed, Nadeem and Khaliq, Saba and Mubarik, Bushra and Abbas, Afia and Jahan, Shah and Afzal, Nadeem}, year={2019}, month={Aug.}, pages={741–747} }