@article{Hoosen_Pool_2018, title={An in vitro study to elucidate the effects of Septilin TM on immune pathways}, volume={17}, url={https://www.banglajol.info/index.php/BJMS/article/view/35877}, DOI={10.3329/bjms.v17i2.35877}, abstractNote={<p><strong><em>Objective: </em></strong>The use of herbal immunomodulatory preparations to prevent and treat immunological complications is increasing in popularity. Rigorous <em>in vitro, in vivo </em>and clinical trial studies are needed to ensure safety, quality and efficacy for the wellbeing of patients. Septilin<sup>TM</sup>, a proprietary herbal medicinal product has been reported to have immunomodulatory effects. This study investigated the <em>in vitro </em>effects of Septilin<sup>TM</sup> on biomarkers of specific immune pathways by using whole blood culture assays (WBC).</p><p><strong><em>Materials and Methods: </em></strong>Stimulated and unstimulated WBC have been incubated with the product. Enzyme linked immunosorbent assays have been used to screen for IL-6, IL-10, and IFN γ as biomarkers for inflammation, humoral immunity, and cell mediated immunity, respectively.</p><p><strong><em>Results: </em></strong>Septilin<sup>TM</sup> had no effect on the release of IL-6 production by (lipopolysaccharide) LPS stimulated WBC across all concentrations tested. However, Septilin<sup>TM</sup> induced significant higher levels (P<0.001) of IL-6 release in unstimulated WBC across all concentrations between 0µg/ml-258µg/ml. Septilin<sup>TM</sup> had no effect on the release of IL-10 release in unstimulated WBC across all concentrations. However the presence of Septilin<sup>TM</sup> in phytohaemagglutinin (PHA) stimulated WBC induced significant higher release (P<0.01) of IL-10 release at concentrations between 64.5µg/ml- 258µg/ml when compared to the control. The presence of Septilin<sup>TM</sup> in unstimulated WBC had no effect on the release of IFN γ production across all concentrations. The presence of Septilin<sup>TM</sup> in PHA stimulated WBC release of IFN γ is inconclusive.</p><p><strong><em>Conclusion: </em></strong>This study shows that Septilin<sup>TM</sup> has immunomodulatory effects on WBC <em>in vitro</em>.</p><p>Bangladesh Journal of Medical Science Vol.17(2) 2018 p.238-244</p>}, number={2}, journal={Bangladesh Journal of Medical Science}, author={Hoosen, Mujeeb and Pool, Edmund John}, year={2018}, month={Mar.}, pages={238–244} }