Bangladesh Journal of Medical Microbiology Official journal of the Bangladesh Society of Medical Microbiologists (BSMM). Full text articles available.<br />BJMM is now accepting online submissions. Please register with the journal and use the 5 step online submission process. Bangladesh Society of Medical Microbiologists en-US Bangladesh Journal of Medical Microbiology 2070-1810 Use of low quality in Vitro Diagnostics in resource poor countries: who will bell the cat? Bangladesh J Med Microbiol 2016; 10 (1): 1-2 Tilovatul Khondaker Nazia Hasan Khan Arifa Akram Nusrat Mannan Saif Ullah Munshi ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 1 2 10.3329/bjmm.v10i1.31430 Detection Of Mutated gyrA Gene From Nalidixic Acid Resistant Salmonella Typhi And Paratyphi A Isolated From Enteric Fever Patients In A Tertiary Care Hospital Of Bangladesh <p>A cross sectional study for the detection of empirically used antibiotic resistant Salmonella was carried out in the department of microbiology of Rajshahi Medical College (RMC) and PCR and DNA sequencing were done in the department of microbiology of Dhaka Medical College during the period from August 2014 to July 2015.Total 323 blood samples were collected from suspected enteric fever patients from medicine and paediatric units of RMC hospital and cultured on brain heart infusion broth for isolation of Salmonella. Identification of Salmonella was done by biochemical tests and final identification was done by specific antisera. Antimicrobial sensitivity test was done by disc diffusion technique. PCR was used to detect gyrA gene. Sequencing of the gyrA gene was done to see mutation. Culture yielded growth of Salmonella typhi in 11 (36.67%) and Salmonella paratyphi A in 19(63.33%) samples. Among 11 Salmonella typhi, 8(72.73%) were resistant to nalidixic acid, 2(18.18%) to ciprofloxacin and one (9.09%) to ofloxacin. Among 19 Salmonella paratyphi A, all (100%) were resistant to nalidixic acid, 4(21.05%) to ofloxacin, and 3 (15.79%) to ciprofloxacin. All the Salmonella typhi and Salmonella paratyphi A were gyrA gene positive. All the nalidixic acid resistant strains of Salmonella typhi and Salmonella paratyphi A were mutated at amino acid position 83 by replacing serine with phenylalanine. None of the nalidixic acid sensitive Salmonella strain had mutated gyrA gene.</p><p>Bangladesh J Med Microbiol 2016; 10 (1): 3-7</p> Maiz-ul Ahad Suman Md. Abdullah Siddique S. M. Shamsuzzaman Abu Rayhan Khandakar Faiz Ahmed Khondaker Sharmin Akter Sumi Roushan Jahan ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 3 7 10.3329/bjmm.v10i1.31431 Detection of Extended Spectrum Beta-lactamase (ESBL) Producing Gram Negative Bacteria from Clinical Specimens of Sir Salimullah Medical College and Mitford Hospital. <p>Detection of Extended spectrum beta lactamase (ESBL) enzyme producing bacteria in hospital settings is vital as ESBL genes are transmissible. This study was carried out to determine the distribution of ESBL producing gram negative isolates at a tertiary care hospital in Dhaka city which deals with the patients hailing from relatively low socioeconomic status.Onehundred and twenty four gram negative bacteria isolated from different clinical specimens from outpatient and inpatient departments of Sir Salimullah Medical College and Mitford Hospital (SSMC &amp; MH) were tested for ESBL by E test ESBL method in the department of microbiology of Sir Salimullah medical college (SSMC) from March 2013 to August 2013.Out of 124 gram negative bacteria 69 (55.65%) were positive for ESBL. Among the ESBL producers, Esch.coli was the highest (46.38%) which was followed by Serratia spp (11.59%), Enterobacter spp (10.14%), Proteus spp, (8.70%), Acinetobacter spp.(7.24%) and Klebsiella spp.(5.79%). Out of 32 Esch.coli isolated from outpatient department, 10 (31.25%) were positive for ESBL. On the other hand out of 27 Esch. coli isolated from inpatient department, 22 (81.48%) were positive for ESBL. The difference was statistically significant (p&lt;0.001).So the present study reveals that the distribution of ESBL producers is more among the hospitalized patients than the patients of the community.</p><p>Bangladesh J Med Microbiol 2016; 10 (1): 8-12</p> Shikha Paul Sanya Tahmina Jhora Prashanta Prasun Dey Bilkis Ara Begum ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 8 12 10.3329/bjmm.v10i1.31435 Antibody Responses In Bangladeshi Children Following Measles Vaccination. <p>Measles is a highly contagious vaccine preventable viral disease which mainly affects children. Infection with wild measles virus induces an immune response that provides life long protection. Measles has been targeted for global eradication. In Bangladesh, there is insufficient data about the antibody responses in children following measles vaccination. In the present study, the antibody response of a single dose of measles vaccine was investigated among 77 children of different age groups. The humoral immune response immunoglobin IgG (IgG) was detected by a commercial Enzyme-linked Immunosorbent Assay (ELISA). Among the study population, detectable antibody titer was observed in 75.3% children while 24.7% showed detectable titers. The mean antibody concentration was highest (2.75 ± 1.10 IU/ml) in the 13-24 months age group, decreased gradually with age, and was lowest (0.77 ± 0.13 IU/ml) in the 85-96 months age group. Thereafter, the mean antibody concentration gradually increased again in the 97-108 months (1.20 ± 0.13 IU/ml) and in the 109-120 months (1.45 ± 0.13 IU/ml) age groups. The mean antibody titer was statistically significant in relation to age (p&lt;0.01) but not to gender (p&lt;0.95). This study showed that around 25% children remained antibody negative indicating challenges ahead for eradication of measles from Bangladesh.</p><p>Bangladesh J Med Microbiol 2016; 10 (1): 13-17</p> Sharmin Sultana Shahina Tabassum Afzalun Nessa Munira Jahan ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 13 17 10.3329/bjmm.v10i1.31447 Isolation of Acinetobacter species and their antimicrobial resistance pattern in a tertiary care hospital in Dhaka, Bangladesh <p>Acinetobacter species are important opportunistic and nosocomial pathogens capable of causing both community and health careassociated infections (HAIs). The clinical specimens obtained from patients admitted in Holy Family Red Crescent Medical College and Hospital (HFRCMCH) from July 2013 to June 2015. All laboratory works were performed in department of microbiology and immunology of HFRCMCH. The infection rate was maximum in blood (67.7%) followed by urine (12.9%), tracheal aspirate (8.8%) and wound swab (3.32%). Most of the Acinetobacter were isolated from Intensive care unit (ICU) and Neonatal intensive care unit ( NICU). Acinetobacter displayed higher resistance to many antibiotics like Ampicillin which showed 96% resistance, Ceftriaxone 72%, Amikacin 72%, Imipenem 55%, Meropenem 60%, Tetraclycline 64%, Ciprofloxacin 60% and Cotrimoxazole showed 38% resistance. In conclusion, there was a high resistant rate to available and common antibiotics. It seems that infection control strategies may help to control the evolving problem of Acinetobacter infections and prevent an epidemic nosocomial life threatening infections.</p><p>Bangladesh J Med Microbiol 2016; 10 (1): 18-21</p> Jannatul Ferdous Mohammad Murshed Sabeena Shahnaz Syeda Sharmin Duza Prothoma Rahman Siddique ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 18 21 10.3329/bjmm.v10i1.31448 Study of Bacterial pathogens in Urinary Tract Infection and their antibiotic resistance profile in a tertiary care hospital of Bangladesh <p>Analyzing antibiotic susceptibility pattern of uropathogens help to overcome the therapeutic difficulties created by the rising antimicrobial resistant bacteria and guides in choosing appropriate antibiotics. Hence, we aimed at evaluating the pathogens causing UTI and study their antibiogram. Midstream urine samples were collected, cultured and appropriate biochemical tests were performed for proper identification of urinary pathogens in BSMMU from January 2013 to December 2013. The most common isolated Gram negative uropathogens were Escherichia coli (63.93%) followed by Klebsiella pneumoniae (17.09%), other bacterial species, named Pseudomonas spp. Enterobacter Acinetobacter spp. Citrobacter spp Proteus spp. Morganella. Among Gram positive organism S. aureus S. saprophyticus S. agalectiae and Enterococci were found. Urinary tract infections were more prevalent in women than men (61.68% vs. 38.32%). High level of sensitivity was found to imipenem, amikacin, nitrofurantoin, ceftriaxone, gentamicin, cefuroxime in most of the isolates. Almost all the test organisms exhibited multiple antibiotic resistances. The high multiple antibiotics resistance identified makes it necessary for antibiotic susceptibility testing to be conducted prior to antibiotic(s) prescription.</p><p>Bangladesh J Med Microbiol 2016; 10 (01): 22-26</p> Sanjida Khondakar Setu Abu Naser Ibne Sattar Ahmed Abu Saleh Chandan Kumar Roy Mushtaque Ahmed Sikder Muhammadullah Md Humayun Kabir ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 22 26 10.3329/bjmm.v10i1.31449 Epidemiology, Transmission, Complications, Laboratory Diagnosis & Management of Zika Virus Infection: A Systematic Review Bangladesh J Med Microbiol 2016; 10 (1): 27-31 S. M. Shamsuzzaman Shahin Ara Begum Sazzad Bin Shahid ##submission.copyrightStatement## 2017-02-13 2017-02-13 10 1 27 31 10.3329/bjmm.v10i1.31450