Bangladesh Journal of Medical Biochemistry

Relation of Serum Bilirubin in Physiological Range with Spot Urine Albumin to Creatinine Ratio and eGFR Among Diabetic Patients with or without Nephropathy

T Tahfim, MA Muttalib, T Rahman, AS Riya, M Alam, M Hasan — Mon, 19 Feb 2024 00:00:00 +0000

Recent studies have suggested a potential role of bilirubin as an antioxidant to protect diabetic patients from hyperglycemia-induced oxidative stress thereby preventing diabetic complications. Diabetic nephropathy is a serious microvascular complication which is determined by increased urinary albumin to creatinine ratio and reduced eGFR. The present work aimed to study the relation of the simple biochemical parameter of serum bilirubin level with the estimated glomerular filtration rate (eGFR) and spot urine albumin to creatinine ratio in diabetic patients with or without nephropathy. This cross sectional study was done in the department of Biochemistry and Molecular Biology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) Academy from July 2017 to June 2018. We have taken 63 diabetic patients without nephropathy and 63 diabetic patients with nephropathy.Data about clinical and biochemical parameters (blood pressure, BMI, fasting serum glucose, estimated GFR, serum bilirubin and spot urine ACR etc.) were measured by appropriate methods. We used student’s t test and Pearson’s correlation coefficient to detect the relationship of serum bilirubin with other variables All statistical tests were considered at 5% level of significance. We found diabetic patients with nephropathy have significantly lower serum bilirubin (p<0.001) than diabetic patients without nephropathy (0.46±0.18 and 0.64± 0.21 mg/d respectively). Pearson’s correlation test showed that serum bilirubin positively correlated with eGFR (r=0.312, p=0.013) in diabetic patients with nephropathy. This study concluded that low serum bilirubin level is associated with eGFR in diabetic patients with nephropathy.

Bangladesh J Med Biochem 2019; 12(2): 16-22

Peripheral Blood Film Patterns in Thalassemia and Hemoglobinopathy Carrier

S Sadiya, WA Khan, B Banu, G Sarwardi, Y Rahman — Mon, 19 Feb 2024 00:00:00 +0000

Thalassemia and hemoglobinopthies are the commonest genetic disorders of hemoglobin. The disease has autosomal recessive pattern of inheritance. Screening of thalassemia and hemoglobinopathy carriers is a burning issue to prevent new cases. Thalassemia and hemoglobinopathies can be prevented by genetic testing followed by genetic counseling, if we can detect carriers of thalassemia or hemoglobinopathies by an easy method. The objective of this study was to see the peripheral blood film pattern among the carriers of thalassemia and hemoglobinopathies. It was a cross-sectional study conducted from September 2015 to November 2015. A total of 437 random specimens were collected from three tribal groups. Two mL of venous blood were collected in EDTA tube. Hemoglobin variants were studied by HPLC method using Variant Hemoglobin Testing System (Variant II Beta Thalassemia Short Program). Statistical analysis was carried out using SPSS statistical package (version 23.0). Data were analyzed by frequency distribution. In this study, total 437 respondents were examined for blood test. Male to female ratio was 1:1. The study included cases from 2 years to 82 years where mean age was 18.3±12.0 years. In clinical analysis, Hb ranged from 8.5 to 19.0 gm/dL and 187 cases were found normochromic, 110 cases anisochromic 140 cases hypochromic. Among them, 38.2% were normal individuals, Hb E Trait were 34.8%, Hemoglobin E disease were 21.7% and Beta Thalassemia trait were 5.3%.Peripheral blood film and hemoglobin status may give an idea about thalassaemia and hemoglbinopathy trait or carrier. In the suspected cases, High Performance Liquid Chromatography (HPLC) may be done to confirm the diagnosis.

Bangladesh J Med Biochem 2019; 12(2): 23-30

Dyslipidemia in Chronic Kidney Disease

N Sultana — Mon, 19 Feb 2024 00:00:00 +0000

Globally chronic kidney (CKD) disease is one of the major health problems. Global prevalence of CKD is 8−16%. Prevalence of chronic kidney disease in Bangladesh is 26%. Patients with chronic kidney disease (CKD) exhibit significant alterations in lipoprotein metabolism. In this review different lipid parameters are shown from different studies and the pathogenesis of CKD induced dyslipidemia is discussed.

Bangladesh J Med Biochem 2019; 12(2): 31-38