Bangladesh Journal of Medical Biochemistry 2018-05-17T12:29:53+00:00 Professor Liaquat Ali Open Journal Systems Official journal of the Bangladesh Society of Medical Biochemists (BSMB). Full text articles available. Point-of-Care Testing (POCT): Importance in Emergency Medical /Health Care 2018-05-17T12:29:11+00:00 ASM Giasuddin <p>Abstract not available</p><p>Bangladesh J Med Biochem 2017; 10(2): 43-44</p> 2018-05-17T12:29:11+00:00 ##submission.copyrightStatement## Enhanced Lipid Profile in Plasma and Erythrocytes of Hypertensive Type-2-Diabetes Mellitus Subjects in South-Western Nigeria 2018-05-17T12:29:15+00:00 AJ Akamo RN Ugbaja OB Onunkwor O Ademuyiwa DA Ojo OA Talabi CA Erinle DO Babayemi DI Akinloye EA Balogun <p>The growing burden of hypertension and type 2 diabetes mellitus (T2DM) in Nigeria and related cardiovascular complications is becoming a public health concern. Cardiovascular risk factors were evaluated in control subjects (n=150) and patients (n=470) [hypertensive nondiabetics (n=179), normotensive diabetics (n=132), hypertensive diabetics (n=159)] attending at the Medical Out-Patient Clinic of the State Hospital, Abeokuta, Nigeria. Cholesterol, triacylglycerols and phospholipids were determined spectrophotometrically in plasma, erythrocytes and lipoproteins. The presence of either or both diseases resulted in significant (p&lt;0.05) perturbations in blood lipids of the male and female patients. Dyslipidemia was characterised by increased concentrations of cholesterol and triacylglycerols in plasma, erythrocytes, low density lipoprotein (LDL) and very low-density lipoprotein (VLDL). The increase was more pronounced in hypertensive diabetics. High density lipoprotein (HDL) cholesterol values of the male and female patients were between 35% to 43% and 37% to 43% respectively lower than their control counterparts, while that of HDL triacylglycerols was between 8% to 10% and 6% to 23% respectively lower than their control counterparts. Plasma and erythrocyte phospholipid content increased significantly (p&lt;0.05) in all the patients when compared with their control counterparts except in the erythrocytes of the normotensive diabetic male, where significant decrease was observed. Our findings suggest that enhanced hypercholesterolemia, hypertriacylglycerolemia and hyperphospholipidemia in plasma and erythrocytes may be responsible for increased cardiovascular complications in the comorbidity since the combined dyslipidemia are more pronounced in comorbidity of hypertension and T2DM than when either of the two conditions occurs in isolation.</p><p>Bangladesh J Med Biochem 2017; 10(2): 45-57</p> 2018-05-17T12:29:15+00:00 ##submission.copyrightStatement## Serum Hepatic Enzymes Level in Type 2 Diabetes Mellitus in a Tertiary Level Hospital 2018-05-17T12:29:25+00:00 K Fatema D Parvin S Islam MSR Hossain MA Rumman F Afroz F Quamrunnahar N Sultana <p>Diabetes mellitus is one of the most common non-communicable diseases globally. Magnitude of DM in developing countries including Bangladesh is rising rapidly. Several studies across the world have reported the association of higher serum hepatic enzyme levels with type 2 DM which may be an expression of excess deposition of fat in the liver. Thus, serum hepatic enzyme levels may serve as a marker of insulin resistance in the liver and may predict the prognosis and complications in type 2 DM individuals. The present crosssectional study was conducted in the Department of Biochemistry, Dhaka Medical College, Dhaka from July' 2015 to June' 2016 to find out the association of serum hepatic enzyme levels with type 2 DM individuals in a tertiary level hospital. A total number of 100 subjects were selected with the age ranging from 30 to 60 years. Among them, 50 diagnosed type 2 DM individuals were included in the Group A and 50 apparently healthy individuals were selected as Group B for comparison. The study parameters were fasting plasma glucose (FPG), HbA<sub>1c</sub>, ALT, AST, ALP and GGT. The mean(±SD) serum ALT, AST, ALP and GGT concentration (U/L) in cases were 46.28±.81, 28.17±10.08, 118.26±16.08 and 36.98±10.08 respectively and mean(±SD) serum ALT, AST, ALP and GGT concentration(U/L) in controls were 29.54±8.56, 24.54±6.89, 96.68±14.36 and 23.82±6.98 respectively. The present study showed that higher levels of serum hepatic enzymes (ALT, AST, ALP and GGT) were present in subjects with type 2 diabetes mellitus. Among these parameters, ALT, ALP and GGT showed positive correlation with FPG and HbA1c in both groups but AST showed no correlation with FPG and HbA<sub>1c</sub>.</p><p>Bangladesh J Med Biochem 2017; 10(2): 58-63</p> 2018-05-17T12:29:25+00:00 ##submission.copyrightStatement## Status of Serum Calcium and Magnesium in Women Taking Oral Contraceptive 2018-05-17T12:29:35+00:00 F Hasanat PK Chakroborty DN Nahar S Bishwash KN Hena R Mollika <p>The oral contraceptive pill fulfills the great human need for birth control with unrivalled effectiveness. The pill can effectively prevent pregnancy and alleviate menstrual disorder when used correctly. Many biochemical profiles of women taking oral contraceptives are disturbed due to metabolic alterations induced by its hormone content. The study was carried out in the Department of Biochemistry, Mymensingh Medical College, Mymensingh, during the period of July 2016 to June 2017 to evaluate the status of serum calcium and magnesium in oral contraceptive user women. For this study, 120 age-matched women were selected and grouped as 60 oral contraceptive user women and 60 non-oral contraceptive user women. Data were analyzed with the help of SPSS version 21. Mean±SD level of serum calcium and magnesium were 9.50±0.70 mg/dl and 1.81±0.13 mg/dl in oral contraceptive user women, while in normal healthy women the levels were 9.03±1.69 mg/dl and 2.10±0.17 mg/dl respectively. No significant difference of serum calcium between two groups was observed (p&gt;0.05). Serum magnesium significantly reduced in oral contraceptive users when compared with non-oral contraceptive users (p&lt;0.001). The study showed no significant difference in serum calcium level in contraceptive group when compared with noncontraceptive user group and serum magnesium level was significantly reduced in oral contraceptive users when compared to normal healthy group.</p><p>Bangladesh J Med Biochem 2017; 10(2): 64-68</p> 2018-05-17T12:29:35+00:00 ##submission.copyrightStatement## Evaluation of LDH and Gamma Interferon as Biochemical Markers for Diagnosis of Pulmonary and Extra-Pulmonary Tuberculosis 2018-05-17T12:29:43+00:00 SK Baul SMR Hossain D Parvin M Hadiuzzaman MS Islam K Fatema <p>Tuberculosis is a common disease throughout the world, especially in developing countries. The most common form of the disease is pulmonary tuberculosis (PTB). Extra pulmonary tuberculosis (EPTB) is also a growing problem worldwide. The diagnosis of PTB and EPTB is even harder when smears and cultures are negative. Due to its nature, the diversity of clinical pictures, the diagnosis of EPTB is often difficult and late. Advances in rapid diagnostic techniques are urgently required both for the early management of the new cases of TB and for the individuals already infected with <em>Mycobacterium tuberculosis </em>and are at risk of developing disease. In addition to standard TB diagnostic techniques, use of new biochemical surrogate markers like LDH and gamma interferon are new suggested. This study was undertaken to evaluate the diagnostic value and accuracy of serum LDH and gamma interferon for the diagnosis of PTB and EPTB. This crosssectional study was carried out in the Department of Pathology and Microbiology, NIDCH, Dhaka and by convenient and purposive sampling, 131 patients (male 98, female 33) having age range of 20-90 years were enrolled for the study. Serum LDH and QuantiFERON-TB gold (QFT-G i.e. gamma interferon) were estimated and the result was compared with the culture and histopathology results. Performance tests were done. Prevalence was measured at 95% CI. Statistical significance was set at p&lt;0.05. Of 131 patients 89 (67.9%) had pulmonary tuberculosis (PTB) and the rest 42 (32.1%) had extra-pulmonary tuberculosis (EPTB). Level of LDH and gamma interferon in serum of all patients was found significantly high. TB pleural effusion was detected in 64% cases. The sensitivity, specificity, positive predictive values, negative predictive values and accuracy of LDH and gamma interferon wre calculated both in PTB and EPTB. For LDH it was (SEN 89.22%, SPE 85.66%, PPV 88.82%, NPV 86.11%) and for gamma interferon (SEN 93.01%, SPE 91.64%, PPV 93.73%, NPV 91.18%) in PTB; and for LDH (SEN 89.27%, SPE 87.18%, PPV 84.35%, NPV 90.36%) and for gamma interferon (SEN 94.88%, SPE 93.65%, PPV 90.04%, NPV 95.82%) in EPTB respectively. When calculated in combination, the sensitivity and specificity was 100% and 50% respectively in both PTB and EPTB, and positive and negative predictive values of LDH and gamma interferon in combination were 94.00% and 91.58% in PTB and 90.43% and 96.71% in EPTB respectively. The study concludes that the diagnostic accuracy of LDH and gamma interferon is low when single test is done but it is significantly high when done in combination.</p><p>Bangladesh J Med Biochem 2017; 10(2): 69-74</p> 2018-05-17T12:29:43+00:00 ##submission.copyrightStatement##