TY - JOUR AU - Moniruzzaman, Mohammad AU - Rahman, Alamgir AU - Hoq, M Mozammel PY - 2008/10/08 Y2 - 2024/03/28 TI - Optimization of Medium Ingredients for Keratinolytic Protease Production by Bacillus licheniformis MZK-03 using Statistical Experimental Designs JF - Bangladesh Journal of Microbiology JA - Bangla. J. Microbiol. VL - 24 IS - 1 SE - Articles DO - 10.3329/bjm.v24i1.1238 UR - https://www.banglajol.info/index.php/BJM/article/view/1238 SP - 52-56 AB - <!--[if gte mso 9]><xml> Normal 0 false false false MicrosoftInternetExplorer4 </xml><![endif]--><!--[if gte mso 9]><xml> </xml><![endif]--><!--[if !mso]><object classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui></object> <mce:style><! st1\:*{behavior:url(#ieooui) } --> <!--[endif] --> <!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} --> <!--[endif] --> <p>A culture medium was optimized for the production of keratinolytic protease by a newly isolated strain of <em>Bacillus licheniformis </em>MZK-03 in shake-flask culture. Based on the results of preliminary experiments, feather mill, molasses and trace elements were found to be major variables in keratinolytic protease production. The concentrations of these ingredients were optimized by using two statistical approaches, namely Box-Wilson method and central composite design. The optimized culture medium, finally determined by using the statistical approaches, composed of 0.95% feather mill, 0.12% molasses and 1.44% trace elements. The keratinolytic protease production was increased by approximately 2-fold when the strain was grown in the optimized medium (95.2 U/ml) compared to the un-optimized medium (56.05 U/ml).</p> <p><strong>Keywords:</strong> Keratinolytic protease, Optimization, <em>Bacillus licheniformis </em>MZK-03, Statistical designs</p><p>DOI: <a href="http://dx.doi.org/10.3329/bjm.v24i1.1238">http://dx.doi.org/10.3329/bjm.v24i1.1238</a> <em></em></p> <p>Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 52-56</p> ER -