TY - JOUR AU - Hoque, M Mahfuzul AU - Rattila, Shemona AU - Shishir, M Asaduzzaman AU - Bari, M L AU - Inatsu, Y AU - Kawamoto, S PY - 2012/09/05 Y2 - 2024/03/29 TI - Antibacterial Activity of Ethanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens JF - Bangladesh Journal of Microbiology JA - Bangla. J. Microbiol. VL - 28 IS - 2 SE - Articles DO - 10.3329/bjm.v28i2.11817 UR - https://www.banglajol.info/index.php/BJM/article/view/11817 SP - 58-63 AB - <p>The ethanol extract of Betel leaf (<em>Piper betle </em>L.), an indigenous climber plant of Bangladesh was screened for its antibacterial activity against some foodborne pathogens viz. <em>Vibrio cholerae </em>ATCC 6395<em>, E. coli </em>ATCC 25922<em>,  E. coli </em>O157:H7 NCTC 12049, <em>Shigella dysenteriae-</em>1 MJ-84 and <em>Staphylococcus aureus </em>ATCC 25923. Experimental  findings revealed that the ethanol extract of betel leaves potentially inhibit the growth of these foodborne  pathogens. Minimum inhibitory concentration (MIC) was ranged from 0.625% (w/v) to 0.75% (w/v). The  ethanol extract of betel leaf showed the highest MIC values for <em>E. coli </em>ATCC 25922 (0.625 mm), <em>Vibrio cholera </em>ATCC 6395 (0.625 mm), and <em>Staphylococcus aureus </em>ATCC 25923 (0.625 mm). It was revealed that the antibacterial  activity was highest at around neutral pH and at moderate temperature. The highest temperature (100°C)  and the pH (10.0) treated ethanol extract showed a little less activity than the maximum activity at moderate  temperature and at neutral pH, suggesting that the ethanol extract of the betel leaf was to some extent high  temperature and high pH tolerant. The ethanol extract of betel leaf might have a potential application as  natural food preservative to improve the microbiological safety of the foods.</p> <p>DOI: <a href="http://dx.doi.org/10.3329/bjm.v28i2.11817">http://dx.doi.org/10.3329/bjm.v28i2.11817</a></p> <p>Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 58-63</p> ER -