Bangladesh Journal of Infectious Diseases <p>Published by the Bangladesh Infection Research Association. Full text articles will be available.</p><p>This new journal invites authors and reviewers to register with the journal. The online submission process is available for new submissions.</p> Bangladesh Infection Research Association en-US Bangladesh Journal of Infectious Diseases 2411-4820 <p class="default"><strong>Copyright</strong></p><p>Copyright on any research article in the Bangladesh Journal of Infectious Diseases is retained by the author(s).</p><p>The authors grant the Bangladesh Infection Research Association a license to publish the article and identify itself as the original publisher.</p><p>Articles in the Bangladesh Journal of Infectious Diseases are Open Access articles published under the Creative Commons CC BY-NC License (<a href=""></a>)</p><p>This license permits use, distribution and reproduction in any medium, provided the original work is properly cited, and it is not used for commercial purposes.</p> Antimicrobial Stewardship: Bangladesh Perspective <p>Abstract Not Available</p><p><span class="fontstyle0">Bangladesh Journal of Infectious Diseases 2018;5(1):1-2</span> <br /><br /></p> Mohammad Abdullah Yusuf ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 1 2 10.3329/bjid.v5i1.37708 Association of HLA DRB1*15 Gene among Acute and Chronic Hepatitis B Infected Bangladeshi Patients <p><strong>Background: </strong>Elucidating differences in HLA DRB1* genes distribution may be useful in understanding the molecular pathogenesis of viral hepatitis B.</p><p><strong>Objective:</strong> The aim of the study was to find out the HLA DRB1*15 gene susceptibility among acute and chronic Hepatitis B infected Bangladeshi patients.</p><p><strong>Methodology:</strong> This cross-sectional study was carried out in the Department of Virology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of June 2012 to June 2013 for a period of one year. Evaluation HLA DRB15*gene distribution was performed among acute hepatitis B and chronic hepatitis B infected (HBV) Bangladeshi patients. HLA DRB15*gene distribution was detected by conventional PCR followed by agarose gel electrophoresis.</p><p><strong>Result:</strong> A total of 60 serologically pre-diagnosed 30 acute hepatitis B and 30 chronic hepatitis B infected (HBV) Bangladeshi patients were evaluated. The study revealed a significant increase of DRB1*15 allele among chronic hepatitis B infected patients compared to acute hepatitis B (46.7% vs 13.3%; RR=5.8, X<sup>2</sup> test=7.2; P&lt; 0.05). This is the first report to investigate HLA DRB1* gene associations among acute and chronic HBV infected Bangladeshi patients.</p><p><strong>Conclusion: </strong>In conclusion HLA DRB1*15 is more frequent in chronic hepatitis B infected Bangladeshi patients compared to acute hepatitis B.</p><p><em>Bangladesh Journal of Infectious Diseases, June 2018;5(1):3-9</em></p> Rokshana Akhter Afzalunnessa Shirin Shahina Tabassum Murad Hossen ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 3 9 10.3329/bjid.v5i1.37709 Bacteriological Profiles of Pus with Antimicrobial Sensitivity Pattern at a Teaching Hospital in Dhaka City <p><strong>Background: </strong>The human skin and soft tissue infections (SSTIs) caused by microbial pathogens during or after trauma, burn injuries, and surgical procedures result in the production of pus, a white to yellow fluid comprised of dead WBCs, cellular debris, and necrotic tissues.</p><p><strong>Objective: </strong>The purpose of the present study was to see the<strong> </strong>frequency and distribution of bacteria isolated from pus and sensitivity pattern.</p><p><strong>Methodology: </strong>This retrospective study was conducted in the Department of Microbiology at Shaheed Suhrawardy Medical College, Dhaka from January 2016 to December 2016 for a period of one (01) year. The pus samples were collected from the patients who were visited in outpatient department and were admitted at IPD in Shaheed Suhrawardy Medical College Hospital (ShSMCH), Dhaka with skin and soft tissue infection. Bacteria were detected by culture and biochemical test and antibiotic susceptibility test done by disc diffusion method.</p><p><strong>Result:</strong> A total number of 212 patients presented with wound infection or pus were recruited for this study. Among 212 patients majority were in the age group of 20 to 40 years which was 89(42.0%) cases. Interestingly male was predominant than female which was 119(56.1%) cases and 93(43.9%) cases respectively. aerobic culture was positive in majority cases which were 131(61.8%) cases.</p><p><strong>Conclusion:</strong> In conclusion the most common isolated bacteria after aerobic culture of pus is the <em>Staphylococcus aureus</em>. However the gram positive cocci is less in number than gram negative bacilli.</p><p><em>Bangladesh Journal of Infectious Diseases 2018;5(1):10-14</em></p> Rashida Akter Khanam Md Rafiqul Islam Ahmed Sharif Rezina Parveen Ishrat Sharmin Md Abdullah Yusuf ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 10 14 10.3329/bjid.v5i1.37710 Evaluation of the Operative Treatment in Chronic Haematogenous Pyogenic Osteomyelitis by Sequestrectomy and Saucerisation in Children <p><strong>Background:</strong> Complete resolution occurs in many other types of infections with the use of antibiotics but not always with chronic pyogenic osteomyelitis.</p><p><strong>Objective:</strong> The aim of the present study was to evaluate the effectiveness of most conventional method of surgical treatment of chronic pyogenic osteomyelitis by sequestrectomy and saucerisation in Children.</p><p><strong>Methodology: </strong>This present study was a prospective study conducted at Bangabandhu Shekh Mujib Medical University (BSMMU), Dhaka, Bangladesh from January 2015 to December 2016 for a period of two (2) years. Patients aged up to 16 years were included in the study. Purposive sampling was done.  Only chronic osteomyelitis of haematogenous origin were included after proper selection of the patients. Sequestrectomy and saucerisation was done. Wound was kept open and allowed to heal from bottom and sides of the wound.</p><p><strong>Result:</strong> A total 36 patients aged up to 16 years were included in the study. Among the cases two third were male 26(72.23%) and rest were female 10(27.77%). The male female ratio was 2.6:1.Therefore the incident was significantly high in males (p&lt;0.01). Most of the patients 28(77.77%) were in 6 to 16 age group, which was significantly high (p&lt;0.01) and mean age was 9.83years. Average time taken for wound healing was 3.75 weeks. Regarding antibiotics, Cap Flucloxacillin was the maximum choice.  Patients were followed up to maximum 2 years and evaluated clinically and radiologically. Maximum patients 31(86.10%) had satisfactory (good and fair) outcome, but only 5(13.88%) patients had poor outcome. There was a significant association among sequestrectomy and saucerisation with final outcome (p&lt;0.001).</p><p><strong>Conclusion:</strong> It was concluded that describing operative technique is still method of choice for surgical treatment of chronic haematogenous pyogenic osteomyelitis.</p><p><em>Bangladesh Journal of Infectious Diseases 2018;5(1):15-20</em></p> Md Mamun or Rashid Md Aminur Rahman AKM Anisuzzaman Choudhury Khursheda Akhtar Khodeza Akhtar ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 15 20 10.3329/bjid.v5i1.37711 Status of Extended Spectrum Beta-Lactamase (ESBL) Producing Bacteria Isolated from Surgical and Burn Wound at Tertiary Care Hospital in Dhaka City <p><strong>Background: </strong>Surgical and burn wound infection are the most common infection in the hospital settings.<strong> </strong></p><p><strong>Objective: </strong>The aim of the present study was to see the status of extended spectrum beta-lactamase (ESBL) producing bacteria isolated from patients presented with surgical and burn wound infection.</p><p><strong>Methodology:</strong> This cross-sectional study was conducted in the Department of Microbiology, BSMMU from January to December 2006, at a period of one year. This study was carried out to detect extended spectrum β-lactamase producing Gram negative bacteria rapidly by using a kit containing chromogenic cephalosporin directly from primary culture by comparison with phenotypic confirmatory method.</p><p><strong>Result: </strong>Total 181 samples were collected from patients with wound infections of which 170(93.9%) bacteria were isolated. Among individual samples ESBLs positive strains were highest in surgical wound which was 22(31.42%) and 24(28.24%) isolates respectively. From surgical wound swab ESBL was found<strong> </strong>3(42.9%) isolates from <em>Klebsiella</em> species. ESBL producing<em> E. coli </em>was found in 12(35.3%) isolates. <em>Pseudomonas</em> species showed in 2(22.2%) isolates and 1(33.3%) isolate of <em>Acinetobactor </em>species. ESBL positive <em>E. coli</em> was found in 5(45.45%) isolates from burn wound. ESBL positive<strong><em> </em></strong><em>Proteus</em> species was detected in 11(28.94%) isolates from burn wound.</p><p><strong>Conclusion:</strong> Most common bacteria isolated from the infected surgical and burn wound are <em>E. coli </em>and <em>Proteus </em>species, though <em>Klebsiella</em> species is the most common ESBL producing bacteria isolated from both infected surgical and burn wound.</p><p><em>Bangladesh Journal of Infectious Diseases 2018;5(1):21-26</em></p> Mostaqimur Rahman Hafiza Sultana Md Abdullahil Mosawuir Latifa Akhter Md Abdullah Yusuf ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 21 26 10.3329/bjid.v5i1.37712 Knowledge of Pulmonary Tuberculosis among the Patients under Anti- Tubercular Therapy <p><strong>Background: </strong>Knowledge on tuberculosis is very important among the patients under anti-tubercular therapy for proper management.</p><p><strong>Objective: </strong>The purpose of the present study was to assess the knowledge about tuberculosis among patients attending the DOTS (directly observed treatment, short course) corner.</p><p><strong>Methodology: </strong>This cross sectional study was conducted in the DOTs corner of Sylhet MAG Osmani Medical College, Sylhet, Bangladesh and Sylhet Chest Disease Hospital, Sylhet, Bangladesh during the period from June 2011 to November 2011 for a period of six (6) months. All the patients who were diagnosed as case of pulmonary tuberculosis and were treated with anti-tubercular drugs at DOTs corner who attended in the both Hospitals, Sylhet were selected as study population.</p><p><strong>Result: </strong>The age of the patients ranged from 18 to 70 years with the mean age of 41.2 (SD<span style="text-decoration: underline;">+</span> 12.4) years. The age of the male patients were ranging from 18 to 70 years with the mean age of 42.1 (SD: 12.8) years. Among the 194 patients, 90(46.4%) patients had good knowledge and 104(53.6%) patients had poor knowledge about tuberculosis.</p><p><strong>Conclusion: </strong>In conclusion, greater efforts therefore need to be undertaken to improve TB control among TB patients through appropriate and sustainable health education.</p><p><em>Bangladesh Journal of Infectious Diseases 2018;5(1):27-31</em></p> Golam Sagir Rafiqul Islam Md Mamnur Rashid Mohammad Akter Hossain Mohammad Ashraful Haque ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 27 31 10.3329/bjid.v5i1.37713 Occult Hepatitis B Virus Infection: A Review Update <p>Occult HBV infection (OBI) is defined as HBV DNA detection in serum or in the liver by sensitive diagnostic tests in HbsAg negative individuals with or without serologic markers of previous viral exposure. Since OBI was first described in the late 1970s, there has been increasing concern in this topic. OBI can be both a source of virus contamination in blood and organ donations and the reservoir for full blown hepatitis after reactivation. HBV reactivation depends on viral and host factors but these associations have not been analyzed thoroughly. Although the exact mechanism of OBI yet not proved, intrahepatic persistence of viral covalently closed circular DNA under the host’s strong immune suppression of HBV replication and gene expression seems to be a cause. Current evidence suggests that OBI can favour the progression of fibrosis, cirrhosis, hepatocellular carcinoma and post transfusion hepatitis (PTH). Epidemiological data regarding the global prevalence of OBI vary due to the use of detection methods of different sensitivity and specificity. Appropriate diagnostic techniques must be adopted. Sensitive HBV DNA amplification assay is the gold standard assay for detection of OBI.</p><p><em>Bangladesh Journal of Infectious Diseases 2018;5(1):32-38</em></p> Arifa Akram ##submission.copyrightStatement## 2018-08-04 2018-08-04 5 1 32 38 10.3329/bjid.v5i1.37714