TY - JOUR AU - Ghasemian, Abdolmajid AU - Peerayeh, Shahin Najar AU - Bakhshi, Bita AU - Mirzaee, Mohsen PY - 2015/09/06 Y2 - 2024/03/29 TI - Biofilm Formation in Multi-Drug Resistant Staphylococcus Aureus Isolates from Hospitalized Patients JF - Bangladesh Journal of Infectious Diseases JA - Bangladesh J. Infect. Dis. VL - 1 IS - 2 SE - Original Articles DO - 10.3329/bjid.v1i2.24902 UR - https://www.banglajol.info/index.php/BJID/article/view/24902 SP - 27-31 AB - <p><span><strong>Background: </strong><span>The biofilm production is an important phenomenon by bacteria such as <span><em>Staphylococcus aureus </em><span>that contribute to the multiple drug resistance. Moreover, biofilm formation by multidrug-resistant <span><em>Staphylococcus aureus </em><span>causes evading from immune responses. </span></span></span></span></span></span></p><p><span><span><span><span><span><span><span><strong>Objective: </strong><span>The aim of this study was to detect <span>biofilm formation and presence of several related genes among multidrug-resistant (MDR) isolates of <span><em>Staphylococcus aureus</em><span>. </span></span></span></span></span></span></span></span></span></span></span></p><p><span><span><span><span><span><span><span><span><span><span><span><span><strong>Methods: </strong><span>This cross sectional study was conducted at a hospital in Tehran, Iran from <span>July 2012 to January 2013. Patients admitted with the infections of the different sites of the body were selected <span>as study population. <span><em>Staphylococcus aureus </em><span>isolates were collected from hospitalized patients and identified by <span>conventional diagnostic tests. The multidrug-resistant MRSA isolates were detected by antibiotic susceptibility <span>test. The phenotypic biofilm formation was detected by micro-titre tissue plate assay. The polymerase chain <span>reaction (PCR) was performed to detect the <span><em>mecA</em><span>, Staphylococcal Cassette Chromosome <span><em>mec </em><span>(SCC<span><em>mec</em><span>) types, <span>accessory gene regulatory (<span><em>agr</em><span>) genes, the <span><em>icaADBC </em><span>and several genes encoding staphylococcal surface <span>proteins including <span><em>clfAB, fnbAB, fib, eno, can, ebps </em><span>and <span><em>bbp </em><span>genes with specific primers. </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><strong>Results: </strong><span>A total <span>number of 209 <span><em>Staphylococcus aureus </em><span>were isolated of which 64 (30.6%) isolates were methicillin-resistant; <span>among which 36(56.2%) isolates were MDR. These isolates were resistant to amoxicillin, tetracycline, <span>ciprofloxacin, gentamicin, erythromycin and trimethoprim-sulfamethoxazole. All the isolates were susceptible <span>to vancomycin and linezolid. All the MDR-MRSA harbored SCC<span><em>mec </em><span>type III. All the MDR- MRSA isolates <span>were strong biofilm producers in the phenotypic test. </span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><span><strong>Conclusions: </strong><span>Multidrug-resistant MRSA isolates <span>produced biofilm strongly and the majority of these isolates harbored most of biofilm related genes.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span><br /><br class="Apple-interchange-newline" /></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>Bangladesh Journal of Infectious Diseases 2014;1(2):27-31</p> ER -