Bangladesh Journal of Botany <p><em>Bangladesh Journal of Botany</em> is the official organ of the Bangladesh Botanical Society established in 1972. Full text articles available.<br>The Bangladesh Journal of Botany is <strong>NOT</strong> accepting online submissions so even if you register with the journal, you will not be able to submit online.</p> en-US <p>© Bangladesh Botanical Society</p><p>Authors are required to transfer the copyright of their articles to the journal. The Declaration form is available here<a href="/index.php/BJB/manager/setup/%20http:/" target="_blank"></a></p> (Prof. Dr. Z N Tahmida Begum) (Md Fahmid Uddin Khondoker) Sun, 28 Oct 2018 17:00:16 +0000 OJS 60 Species diversity, change in forest cover and area of the Sundarbans, Bangladesh <p>The phytosociological analysis, species diversity and types, fidelity, presence, constancy, Sørensen similarity index and changes in forest cover, land and water areas since 1972 were analyzed. Forty six species were recorded both in quadrats and growing outside which belong to 26 families and 41 genera, 19 were distinguished as true mangrove species and the rest were mangrove associates. Leguminosae and Rhizophoraceae were the dominant families represented by 5 spp. each. Three species were found to be invasive, namely <em>Blumea lacera </em>(Burm. F.) DC., <em>Catharanthus roseus </em>(L.) G. Don., <em>Wedelia chinensis </em>(Osbeck) Merr. The maximum importance value index was found in <em>Heritiera fomes </em>Buch.-Ham (48.08). The overall species diversity (H) of SMF was found 3.81, species richness (d) was 9.10 and evenness (e) was 0.47. The forest area was 385,237 ha in 2015. The satellite images showed that the forest and water area of SMF decreased gradually till 2015 in comparison to 1972. The bare land of Sundarbans showed a significantly increasing trend till 2015 since 1972.</p> Ashfaque Ahmed, Md Ataullah, Parveen Rashid, Ashit Ranjan Paul, Sukumar Dutta, Md Shahjahan Ali ##submission.copyrightStatement## Sun, 28 Oct 2018 16:57:42 +0000 Response of guava (Psidium guajava L.) softwood cuttings to paclobutrazol application in different rooting media <p>The scope of clonal propagation of guava (<em>Psidium guajava </em>L.) by soft wood cuttings collected from mature plant is described. The most successful media among those traditionally used and to identify promising alternatives was determined. Soft wood cuttings were done to determine the most successful medium and Paclobutrazol (PBZ) hormone concentration. Guava soft wood cuttings were treated with 0, 200, 400 and 600 mg/l PBZ solution and planted in three different rooting media (sand, silt and top soil) and rooted in low-tunnel. The cuttings were allowed to grow under low-tunnel for three months to assess the rooting capability and shooting competency. The study revealed that the species has a great potential for clonal propagation through soft wood cuttings.</p> Rashad Qadri, M Tahir Akram, Imran Khan, Muhammad Azam, Numrah Nisar, M Awais Ghani, Mohsin Tanveer, MM Khan ##submission.copyrightStatement## Sun, 28 Oct 2018 16:57:51 +0000 In vitro slow-growth conservation for two genotypes of Solanum tuberosum L. <p>To find out the most suitable culture medium formulation to induce slow-growth and reduce the frequency of sub-culturing of the <em>in vitro </em>conserved microplants of the two potato genotypes at 24 ± 1ºC was conducted. Growth was controlled by using different concentrations of sucrose, mannitol, sorbitol (30 g/l) alone or in combination with either mannitol (15, 20 and 25 g/l) or sorbitol (15, 20 and 25 g/l) in murashige and Skoog medium. The results showed that single treatment (Sucrose or mannitol or sorbitol alone) was not feasible for long-term conservation. Combined treatment was responding better and maximum microplant survived (80.82 - 83.15%) after 12 months of storage on (T-8) medium supplemented with 10 g/l sucrose and 20 g/l sorbitol. In this formulation microplants were in very good condition, without phenotypic abnormalities and had enough nodes for sub-culturing up to 12 months. Microplant survival and condition were closely associated with each other but not with root growth.</p> M Nasiruddin, AKM Rafiul Islam ##submission.copyrightStatement## Sun, 28 Oct 2018 16:57:59 +0000 Screening of wheat genotypes against salinity at early vegetative stage in pot culture <p>Experiment was carried out to investigate intraspecific variation for salt tolerance and to classify the wheat genotypes into different salt tolerant groups. Considerable variations among the genotypes in response to salinity were observed for shoot length under 16 dS/m NaCl salinity in the both seasons. Salt tolerant genotype was found to be less affected at high salinity and could be produced better total dry matter compared to other genotypes. Five and seven genotypes appeared as tolerance during 2008 - 09 and 2009 - 10, respectively based on relative total dry matter (RTDM). On the other hand, six and three genotypes exhibited tolerant during 2008 - 09 and 2009 - 10, respectively based on visual scoring. The distribution pattern of the genotypes into various salinity tolerant groups remained fairly constant under two methods. Three genotypes G24, G33 and G40 exhibited tolerant category. RTDM compared to the control would be very useful trait in salinity tolerant improvement programme. However, visual scoring provide guidelines for mass screening of salt tolerant genotypes.</p> MS Uddin, KMW Hossain ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:04 +0000 Mycoflora associated with post-harvest disease of papaya (Carica papaya L.) and their pathogenic potentiality <p>Nineteen species of fungi namely, <em>Alternaria alternata </em>(Fr.) Keissler, <em>Aspergillus flavus </em>Link, <em>A. fumigatus </em>Fresineus, <em>A. niger </em>Van. Tieghhm, <em>Colletotrichum dematium </em>(Pers.) <em>ex</em>. Fr., <em>C. gloeosporioides </em>(Penz.) Sacc., <em>Corynespora citricola </em>M.B. Ellis, <em>Curvularia lunata </em>Wakker, <em>Fusarium flocciferum </em>Corda, <em>F. nivale </em>(Fr.) Ces., <em>Fusarium </em>Link, <em>Lasiodiplodia theobromae </em>(Pat.) Griff &amp; Moubl, <em>Monilia </em>Pers., <em>Mucor </em>Fresen, <em>Penicillium </em>Link<em>, Pestalotiopsis guepinii </em>(Desm.) Stay., <em>Rhizoctonia solani </em>J.G. Kuhn, <em>Rhizopus stolonifer </em>Bull. and <em>Syncephalastrum </em>Schroet were found to be associated with the diseased fruits of <em>Carica papaya </em>L. Among the isolated fungi <em>C. gloeosporioides, F. nivale </em>and <em>Fusarium </em>sp. were found to be pathogenic for both red and yellow cultivar Shahi papaya. Association of <em>C. lunata</em>, <em>F. nivale</em>, <em>P. guepinii </em>and <em>Syncephalastrum </em>sp. with papaya is a new record. The present report is the first record of <em>Corynespora citricola </em>and <em>Fusarium flocciferum </em>from Bangladesh.</p> Resuana Binte Helal, Sarowar Hosen, Shamim Shamsi ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:10 +0000 Simulation of the penetration range distribution for low energy ions injected into plant seeds <p>In the present experiment on low-energy ions implanted into seeds, factors such as the unique microstructure of the seeds, the energy and injection mode of the ions, the step diameter and scattering angle of the ions after collision, and the collision energy loss between ions and atomic nuclei or electrons are considered. A modified model for ions implanted into seeds is established. The range distribution is simulated for vanadium, titanium, and iron ions (V<sup>+</sup>, Ti<sup>+</sup>, and Fe<sup>+</sup>) with different energies and injection modes implanted into peanut, cotton, and wheat seeds, using the FORTRAN programming language and Monte-Carlo method; the results are in good agreement with the experimental data. In addition, the model is used to estimate the range distribution for nitrogen, hydrogen, and argon ions (N<sup>+</sup>, H<sup>+</sup>, Ar<sup>+</sup>) implanted into peanut, cotton and wheat seeds.</p> Wang Linxiang ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:17 +0000 Intervarietal variation in salt tolerance of lentil (Lens culinaris Medik.) in pot experiments <p>Seven lentil (<em>Lens culinaris </em>Medik.) varieties were grown in pots irrigated with NaCl solution of different concentrations (0, 100, 200 and 300 mM) to assess their salinity tolerance potentials. Data revealed that the highest and the lowest salt tolerance were shown by BARI masur-5 (168.50%) and BARI masur-2 (56.32%), respectively. Cluster analysis based on the salt tolerance indices also showed grouping of the varieties into 4 clusters where BARI masur-5 was found highly salt tolerant, BARI masur-6 was moderately salt tolerant and BARI masur-1 and BARI masur-2 were least tolerant. Although shoot height, fresh weight and water content decreased, root length and root to shoot ratio increased significantly with the increase of salt concentrations in the varieties tested.</p> Mohammad Zabed Hossain, Md Mehedi Hasan, Md Abul Kashem ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:22 +0000 Insecticidal activity of essential oil from seeds of Poncirus trifoliata (L.) Raf. <p>The essential oil from the seeds of oriental medicinal plant <em>Poncirus trifoliata </em>(L.) Raf. was tested for repellent and fumigant efficacy on fruit fly (<em>Drosophila melanogaster</em>) and mosquito (<em>Anopheles culicifacies</em>). The oil showed potent activity with 76.2 and 93.2% mortality in fumigant assay, and 77.1 and 93.2% repellent efficacy on fruit fly at doses of 100 and 200 μg/ml, respectively. The application of oil against mosquito showed 80.0% mortality in fumigant assay and 70.3% repellency at 100 μg/ml dose. Thus, essential oil of <em>P. trifoliata </em>seeds can be considered as a potential source of biologically active compounds for pest control.</p> Atiqur Rahman, Shah Alam Siddiqui, M Oliur Rahman, Sun Chul Kang ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:28 +0000 Effects of drought stress on growth, grain filling duration, yield and quality attributes of barley (Hordeum vulgare L.) <p>A pot experiment was conducted to investigate the effect of drought stress at start of anthesis (applied by adjusting the field capacities at 100, 50 and 30%) on barley growth, grain filling duration, grain shape, yield and quality attributes. The effect of drought stress was more prominent on plant fresh biomass accumulation, grain yield and grain filling duration. However, it produced non-significant effect on total number of tillers and grain protein contents. With the increasing intensity of drought stress, barley growth and yield traits significantly diminished. Water stress gradually shortened the plant height and biomass accumulation but the difference was more prominent in fresh biomass accumulation (– 45%) over dry biomass accumulation. The field capacity of 30% caused 29 - 41% reduction in leaf chlorophyll content and 10 - 27% in grain quality traits. Root fresh and dry biomass accumulation decreased by drought stress while root length increased. Drought stress produced uneven grain size that resulted in lower grain yield (42%) specially at 30% field capacity. This reduction in yield was also due to the decreased grain filling duration (38 d) at 30% field capacity as compared to 100% field capacity. So, it may be concluded that drought stress affected barley yield through impaired grain development and grain filling duration. The results of present study are satisfactory and needed further exploration about the physiological mechanism and management strategies to overcome drought stress related yield losses in barley crop.</p> Fahad Alghabari, Muhammad Zahid Ihsan ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:34 +0000 Antioxidant, anti-urease and anti-elastase activities of Usnea longissima Ach. <p>Lichens are complex associations composed of mycobiont and one or more algae or cyanobacteria which are living in symbiosis. Lichens are thought to possess therapeutic effects on many illnesses in worldwide. This study was performed to investigate antioxidant, anti-elastase and anti-urease activities of <em>Usnea longissima </em>Ach lichen. <em>U. longissima </em>was extracted with ethanol and ethyl acetate solvents. The antioxidant activities of the extracts of <em>U. longissima </em>were determined with methods such as 1,1-diphenyl-2- picrylhydrazyl <strong>(</strong>DPPH) radical scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, copper reducing antioxidant capacity (CUPRAC), total antioxidant capacity, determination of total phenolic content and total flavonoid contents. The binding action of the DPPH and ABTS radicals, CUPRAC activity increases with concentration of the extracts. Ethanol extracts exhibited higher anti-urease and anti-elastase activity. Highest inhibition was found as 49.86% for elastase and 18.38% for urease. Results of the present study suggest that <em>U. longissima </em>extracts can be an alternative to synthetic antioxidant, anti-elastase and anti-urease agents.</p> Sinem Aydin, Kadir Kinalioğlu, Bahar Bilgin Sökmen ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:41 +0000 Genetic variabilities and relationships among some egyptian Aspergillus spp. based on microscopic characters, isozyme profiles and random amplification of polymorphic DNA <p>Twelve <em>Aspergillus </em>spp. were isolated from the Egyptian soils and were studied morphologically and microscopically. Isozyme profiles and random amplified polymorphic DNA (RAPD) were also employed for determination of the genetic relationship among the tested <em>Aspergillus </em>spp. Results of RAPD revealed genetic similarity level above 80% between <em>A. terreus </em>and <em>A. aculeatus</em>. The species <em>A. niger</em>, <em>A. candidus</em>, <em>A. terreus </em>and <em>A. aculeatus </em>were joined with a similarity level of 65%. Unique bands were displayed by certain fungi and can be taken as a positive marker for isolate identification. Five isozyme systems; peroxidase, alcohol dehydrogenase, α esterase, β esterase and superoxide dismutase were studied to detect the genetic variabilities among the tested isolates. Subsequently, comparing the three dendrograms which belong to morphological characters, isoymes and RAPD revealed a great relationship between <em>A. terreus </em>and <em>A. niveus </em>from one side and <em>A. candidus </em>and <em>A. terreus </em>from the other side<strong>.</strong></p> Elham Gaber, Manal M Hosseiny, Heba I Abo-Elmagd ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:48 +0000 Seed mucilage content in Aethionema W.T. aiton species and their significance in systematic and ecological aspects <p>The seeds of the seven <em>Aethionema </em>taxa, namely <em>A. syriacum </em>(Boiss.) Bornm., <em>A. froedinii </em>Rech., <em>A. arabicum </em>(L.) Andrz. ex DC<em>.</em>, <em>A. speciosum </em>Boiss. et Huet<em>. </em>subsp. <em>speciosum, A. saxatile </em>(L.) R. Br.<em>, A. armenum </em>Boiss. and <em>A. grandiflorum </em>Boiss. et Hohen. were found to have mucilage cells on the surface, and they produce a slippery liquid during hydration. The mucilage in the examined taxa consisted of the pectin or cellulose. Mucilage cells were found to have different anatomical layers in seeds. Moreover, there were differences in columellae shapes in <em>Aethionema </em>taxa, which are prominent, flattened or reduced shapes. In addition, soil adhesion capacities of the taxa ranged from 28 to 356 mg. The presence of mucilage can play a key role in seed dispersion and colonization for the new habitat in <em>Aethionema </em>taxa.</p> Mehmet Cengiz Karaismailoglu ##submission.copyrightStatement## Sun, 28 Oct 2018 16:58:54 +0000 Determination of correlation between plant distribution and ecological factors in Narowal district Punjab, Pakistan <p>In order to study correlation between ecological factors and vegetation distribution, multivariate analysis was undertaken. Thirty four plant families represented by 59 species were recorded. For classifying the plant communities TWINSPAN (Two Way Indicator Species Analysis) software program was used. After analysis plants were categorized into two large (major) and six small (sub) communities. Water and soil samples were analyzed for pH, EC, soil colour and soil water content. Canonical correspondence analysis (CCA) was employed to correlate species distribution pattern to environmental factors. Results showed that despite many variables being strongly correlated, they were of little value in grouping together of species. However, soil pH and electrical conductivity (EC) had effects on distribution of vegetation.</p> Arifa Zereen, Sheik Saeed Ahmad, Zaheer-Ud-Din Khan, Alman Jahan ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:00 +0000 Fruit, seed and pollen morphology of Chorispora DC. species (Brassicaceae) of Turkey <p>Detailed description of fruit, seed and pollen macro- and micromorphological characters of Turkish <em>Chorispora </em>species are provided with illustrations. Typical fruits are linear, straight or strongly curved upward. Nonglandular and glandular trichomes are present or absent. Seeds varied in shape from oblong, oblong-broadly elliptic to subglobose and winged at the apex and base or not. The pollen grains are tricolpate and the basic shape of the pollen grains in species studied is perprolate. The surface sculpturing type is reticulate. Among the studied characters, fruit, seed size and colour, seed shape, fruit trichome structure and pollen size were of taxonomic importance and useful in separating taxa.</p> Fatih Satil, Ayla Kaya, Murat Ünal ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:05 +0000 Effects of organic formulations and synthetic fertilizer on the performance of pigeonpea in eastern region of Uttar Pradesh <p>A field experiment involving five organic formulations <em>viz</em>., cow urine, vermiwash, neem seed extract, fish wash normal water (control) and three levels of NPK <em>viz</em>., 50% recommended dose of fertilizer (RDF), 75% RDF and 100% RDF with total 15 treatment combinations in randomized complete block design with three replications was conducted at Varanasi during <em>Kharif </em>season (July-April) 2014-15 to evaluate the effect of different organic formulations and NPK fertilization on pigeonpea. Results showed that increasing level of NPK up to 100% RDF significantly improved growth parameters, yield attributes, grain and straw yield. Among the treatment combinations, combined application of 100% RDF + vermiwash proved superior over other treatments, these recorded the highest growth yield attributes, gross returns, and net returns while B : C ratio was the highest under 100% RDF in combination with cow urine.</p> Sudhanshu Verma, Abhishek Singh, Swati Swayamprabha Pradhan, JP Singh, SK Verma ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:11 +0000 Pectinase production from Aspergillus niger IBT-7 using solid state fermentation <p>Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as <em>Aspergillus niger </em>IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).</p> Roheena Abdullah, Iqra Farooq, Afshan Kaleem, Mehwish Iqtedar, Tehreema Iftikhar ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:18 +0000 Effects of smoke-water and smoke-derived butenolide on accumulation of phenolic acids in cultured hairy roots of Salvia miltiorrhiza Bung <p>Smoke-water (SW) and biologically active compound butenolide (KAR1) isolated from smoke show stimulating effects on the plant growth of agricultural and horticultural crops, while little has been known on their effects on the secondary metabolism of medicinal plants. Hairy root culture of <em>Salvia miltiorrhiza</em>, an important medicinal plant, was treated with SW and butenolide. The results showed that the contents of salvianolic acid (SAB) and rosmarinic acid (RA) were enhanced with the treatments of SW and KAR1. The expressions of genes (PAL, C4H, 4CL, TAT and HPPR) involved in phenolic acids biosynthesis were upregulated by treatments of SW and KAR1. Responses of C4H and 4CL to SW and KAR1 treatment were later than PAL, TAT and HPPR. It suggests that SW and KAR1have the potential to be used as elicitor to increase the accumulation of secondary metabolites in <em>S. miltiorrhiza </em>hairy root.</p> Jie Zhou, Zixin Xu, Zhifang Ran, Lei Fang, Lanping Guo ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:25 +0000 Bromatological and sensory analyses of a snack based corn flour and cassava root fortified with moringa to combat the malnutrition <p>This research details an effort to develop a nutritious snack that can alleviate, and combat problems associated with undernourishment product was developed using corn, cassava, and moringa (or teberinto). Two samples of the product were made, with 1 and 2% moringa, along with corn (60%) and cassava (39%) and were tested through a sensory analysis with 15 panelists. The better product was determined by applying the ANOVA to the results of the sensory analysis. The selected sample was the one containing 1% moringa. The product was subjected to microbiological tests for quality and bromatological tests and to ascertain its chemical composition. Results from both tests met the requirement of established standards. Three physicochemical determination (protein, moisture and ash) also provided favorable results. Thus combining these foods into a snack may contribute to mitigation of undernourishment.</p> Juan Carlos Calderon Lopez, Kampanad Bhaktikul ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:32 +0000 Effects of soil enhancer (XXL) on yield attributes of BRRI dhan29 and hybrid dhan TAJ1 cultivars of rice in boro season <p>The present study used two varieties (BRRI dhan29 and Hybrid dhan Taj1) of rice and (control) and six different concentrations of soil enhancer XXL [125% (1.88/1.5 g/l), 100% (1.5/1.5 g/l), 75% (1.125/1.5 g/l), 50% (0.75/1.5 g/l), 33% (0.50/1.5 g/l) and 25% (0.38/1.5 g/l)] with recommended doses of (N, P, K, S and Zn) fertilizers. Significant variation was observed in different yield contributing characters with soil enhancer (XXL) application. Among the different soil enhancer (XXL) concentrations, 75% XXL provided the highest yield (10.58 t/ha). With 75% XXL, BRRI dhan29 and Hybrid dhan Taj1 provided their highest grain yield (11.09 t/ha and 10.07 t/ha, respectively). However, BRRI dhan29 performed well due to yield attributes (fertile tillers/hill, panicle length and shoot dry matter/hill which were 11.94, 23.95 cm and 21.59 g, respectively) than that of Hybrid dhan Taj1 with 75% XXL applied.</p> Md Saidur Rahman, Md Moinul Haque, Kamal Uddin Ahamed ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:37 +0000 Estimation of broad-sense heritability and variance components for seed yield and agronomic traits in native and exotic safflower (Carthamus tinctorius L.) genotypes <p>This study was conducted to estimate broad-sense heritability and variance components for seed yield and some agronomic traits in 100 safflower genotypes. The experiments were carried out in two years at two locations in Iran. The number of seeds per capitol and harvest index had the highest genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV), respectively. The estimated broad-sense heritability for plant height, days to physiological maturity, 1000-seed weight, days to flowering, number of seeds per capitol, number of capitols per plant and seed yield were 86, 76, 74, 70, 68, 44 and 16%, respectively. It was found that plant height, days to physiological maturity, 1000-seed weight, days to flowering and number of seeds per capitol were the least influenced by the environment. The number of capitols per plant and seed yield were most affected by the environment.</p> Zahra Tahernezhad, Jalal Saba, Mehrshad Zeinalabedini, Seyyed Safid Pourdad, Mohammad Reza Ghaffari ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:43 +0000 Effects of ga3 and shoot pruning on flowering and yield in Assam lemon (Citrus limon Burm. f.) <p>Effect of GA3 and pruning on flowering and yield in Assam lemon (<em>Citrus limon </em>Burm. F.) were studied. Ten treatments were imposed for two seasons (summer and winter) in which significant among the treatments were found for profuse flowering and its yield. Among the different treatments, the pooled data of two seasons showed that G<sub>1</sub>P<sub>1</sub> (GA<sub>3</sub> 50 ppm single spray plus 15 cm shoot pruning) recorded the highest flower per branch (29.75.), fruitlets per branch (6.4) and yield (10.45 t/ha) as compared to the control plants 26.5 flowers per branch, 5.8 fruitlets per branch and 9.43 mt/ha, respectively. Therefore, combination of GA3 50 ppm single spray + 15 cm shoot pruning is most promising which can be recommended for improvement of yield in lemon cv. Assam lemon.  </p> M Mahesha, SR Singh ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:50 +0000 High cadmium uptake ability of Bacillus cereus strains isolated from rhizosphere of Tagetes minuta L. growing in cadmium-polluted soil <p>Microbes resistant to heavy metals develop mechanisms to accumulate Cd(II) in their cells. Two bacterial strains, <em>Bacillus cereus </em>AVP12 and <em>B. cereus </em>NC7401 which grew at high Cd(II) concentration were isolated from roots of <em>Tagetes minuta </em>L. growing in Cd(II) contaminated and uncontaminated soil. Minimum inhibitory concentration (MIC) and percent removal capacity were determined as function of pH, contact time and initial Cd(II) concentration. Bioaccumulation capacity was determined to observe possible effect of two different rhizospheres on Cd(II) removal capacity of both strains. Both strains were resistant up to 300 mg/l Cd(II) concentration. The percent removal capacity of both strains was maximum at pH 7 and incubation time of 24 hrs. High bioaccumulation capacity was observed with increasing Cd(II) concentration. Both Langmuir and Freundlich models fitted well to data of Cd(II) bioaccumulation. Though, maximum adsorption capacity (Q<sup>o</sup>) was observed for strains isolated from both types of rhizospheres, however remarkable Q<sup>o</sup> values of 434.0 and 212.7 mg/g were observed for <em>Bacillus cereus </em>AVP12 and NC7401, respectively isolated from polluted rhizosphere. <em>Bacillus cereus </em>strains growing in polluted rhizosphere can develop high Cd(II) uptake ability in comparison to non-polluted rhizosphere.</p> Kulsoom Akhter, Tahseen Ghous, Muhammad Siddique Awan, Zain Ul-Abdin, Basharat Hussain ##submission.copyrightStatement## Sun, 28 Oct 2018 16:59:55 +0000 Use of algae from an oasis in Saudi Arabia in production of biofuel and bio-fertilizer <p>AlAhsa oasis in Saudi Arabia is one of the largest oases in the world. Algae, from this region have been under-explored in the past decades. A study was conducted with <em>Chlorococcum </em>strain to produce biofuel alongside the seaweed <em>Hormophysa cuneiformis</em>. Gas Chromatography/Mass Spectrometry of fatty acid composition showed that the biodiesel obtained had limited number of unsaturated fatty acids as compared to the number of saturated fatty acids present, which indicates the stability of the produced biodiesel. Thereby the use of algal biomass for the production of biofuel is feasible. Moreover, the biomass may serve other different biotechnological applications. To further test this hypothesis, the aqueous extract of two different algae; one derived from the blue green alga (cyanobacterium) <em>Phormidium </em>sp. and the other from brown alga <em>Hormophysa cuneiformis </em>was used as liquid biofertiliser at concentrations of 50 and 10% of both algae. Sterilized <em>Vigna </em>seeds were soaked in the extracts for two days. Seeds were sown in sterilized soil and the germination percentage as well as shoot and root lengths were recorded for developing seedlings. The results showed that there was a significant increase in seed germination rate compared to control. Similarly, there was a significant increment in the length of root and root system compared to control with the 50% aqueous extract concentration being highest in growth parameters for brown alga followed by blue-green alga possibly due to the presence of growth stimulants in these extracts.</p> Nermin Adel El Semary, Howrah Mahdi, Abrar Alnoaim, Kawther Heji Alsofan, Sarah Ibrahim Almsthi, Wed Saleh Albader ##submission.copyrightStatement## Sun, 28 Oct 2018 17:00:03 +0000 Use of UV-C radiation for reducing storage losses of potato tubers <p>Ultraviolet radiation in the C band to limit storage losses of potato was applied. Six edible potato varieties, <em>viz</em>., Lord, Vineta, Owacja, Ditta, Finezja and Tajfun were used as test materials. UV-C radiation was emitted from a 15 W radiator. The wavelength of the light used to irradiate potato tubers was 253,7 nm and the power density from 80 to 100 μW/cm<sup>2</sup>. The method of reduction of storage losses of potato tubers, based on their exposure to UV-C irradiation, can complement the presently used methods of potato crop protection during long-term storage.</p> Tomasz Jakubowski ##submission.copyrightStatement## Sun, 28 Oct 2018 17:00:11 +0000