TY - JOUR AU - Banu, Tanjina Akhtar AU - Khan, Salim AU - Goswami, Barna AU - Afrin, Sadia AU - Habib, Ahashan AU - Akter, Shahina PY - 2020/12/31 Y2 - 2024/03/28 TI - Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants JF - Bangladesh Journal of Botany JA - Bangladesh J. Bot. VL - 49 IS - 4 SE - Articles DO - 10.3329/bjb.v49i4.52534 UR - https://www.banglajol.info/index.php/BJB/article/view/52534 SP - 1021-1027 AB - <p>Highest frequencies (75%) of friable embryogenic nodular calli were recorded from the root explants in the presence of MS + 4.0 mg/l BAP and 4.0 mg/l NAA. In combinations of BAP and NAA 45.4% callus produced direct plantlets formation and 36.3% only from aerial part of shoots. MS medium with 1.0 mg/l BAP and 0.5 mg/l Kn produced compact callus where 13.3% produced direct plantlets formation and 45.7% produced only shoot formation. Well-developed globular somatic embryos were found to form when the callus were cultured more than 6 - 8 weeks on MS with 1.0 mg/l BAP and 0.5 mg/l Kn. After acclimatization the well rooted plantlets were transferred to pot and grown successfully in soil.</p> ER -