@article{Khan_Goswami_Akter_Islam_Noon_Habib_Banu_2019, title={In vitro mass propagation of Piper betle L}, volume={48}, url={https://www.banglajol.info/index.php/BJB/article/view/47917}, DOI={10.3329/bjb.v48i3.47917}, abstractNote={<p>An efficient <em>in vitro </em>regeneration system was developed for <em>Piper betle </em>L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best response on shoot/explants 13.2 ± 4.5 after 8 weeks of callus culture on MS medium with 0.5 mg/l BAP. The best response towards root induction was observed on half strength of MS with 0.25 mg/l IBA. The well rooted plants were successfully acclimatized and transferred to soil.</p>}, number={3}, journal={Bangladesh Journal of Botany}, author={Khan, Salim and Goswami, Barna and Akter, Shahina and Islam, Mousona and Noon, Afsana Huq and Habib, Ahashan and Banu, Tanjina Akhtar}, year={2019}, month={Sep.}, pages={559–566} }