Bangladesh Journal of Animal Science Official journal of the Bangladesh Animal Husbandry Association, Bangladesh Agricultural University, Mymensingh Bangladesh Animal Husbandry Association en-US Bangladesh Journal of Animal Science 0003-3588 <p>© Bangladesh Journal of Animal Science.</p><p>Authors are required to transfer their copyright to the Bangladesh Journal of Animal Sciences.</p><p>All rights reserved. No parts of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise without the prior permission of the Bangladesh Journal of Animal Science.</p> Effect of garlic powder (Allium sativum) on growth, dressing parameters, serum biochemical contents and profitability of broiler <p>This study was conducted to evaluate the effect of garlic an alternative feed additive in broiler diet.  The experiment was carried out for a period of 32 days with a number of 340 day-old straight run broiler chicks. Birds were divided into five dietary treatment groups with 4 replications each having 17 birds. The dietary groups were; control (basal diet; no additives), antibiotic (basal diet + antibiotic), garlic 0.25% (basal diet + 0.25% garlic powder), garlic 0.50% (basal diet + 0.50% garlic powder) and garlic 0.75% (basal diet + 0.75% garlic powder). Results showed that the body weight, body weight gain and total FCR were not differ significantly (P&gt;0.05) among the treatment groups. Total feed intake in different dietary levels showed significant (P&lt;0.05) difference. Control group showed significantly (P&lt;0.05) higher feed intake than the other dietary groups. The results also showed thatthe serum glucose concentration was reduced (P&lt;0.05) in all garlic supplemented groups compared to the control and antibiotic groups. Total cholesterol level was significantly (P&lt;0.05) lower in the 0.75% garlic group compared to the control and antibiotic groups. In case of dressing parameter, 0.75% garlic group showed lowest (P&lt;0.05) abdominal fat compared to the other groups. Cost of production per kg live broiler was lower in antibiotic and control groups compared to the garlic group. With regards to profit, antibiotic groups showed higher profitability than the other groups. It can be concluded that the addition of garlic powder to broiler diet had positive effect on cholesterol and glucose levels and had no effect on feed intake, body weight gain, meat yield, bone development, carcass parameters. It can be suggested that the garlic could be a potential feed additive in broiler diet.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>215-224</em></strong></p> MB Karim ME Hossain MS Ali A Hossain ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 215 224 10.3329/bjas.v46i4.36961 Effect on blood cholesterol level with increase in concentration of probiotics to Giriraja and Sakini chicken in Nepal <p>The chicken population is increasing and the meat is acceptable by most ethnic groups, these species are remarkable for the meat production in the country. Probiotic microorganisms have played significant role in lowering cholesterol which have been documented over the past two decades via various clinical trials. This Cholesterol lowering properties of microorganism have been proposed to deconjugate bile via production of bile salt hydrolase (BSH) associated with their cholesterol lowering potentials in prevention of hypercholesterolemia. Deconjugated bile salts are more hydrophobic than their conjugated counterparts, thus are less reabsorbed through the intestines resulting in higher excretion into the feces. Of total 16 replicate, T1R1-T1R4 were without probiotics whereas T2R1-T2R4, T3R1-T3R4, T4R1-T4R4 were with probiotics 5%, 10% and 15% concentration Probiotics for Sakini male and female. Similary for Giriraja breed T5R1-T5R4, T6R1-T6R4, T7R1-T7R4 and T8R1-T8R4 were 0%, 5%, 10% and 15% concentration of with Probiotics. There was comparatively degradation of Cholesterol in blood with increase in probiotics concentration via Cell Net Analyzer.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>225-229</em></strong></p> D Neupane DB Nepali NR Devkota MP Sharma IP Kadariya ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 225 229 10.3329/bjas.v46i4.36962 Effect of chitosan on quality and shelf life of beef at refrigerated storage <p>In this study, the microbiological quality and shelf life of beef treated with different concentrations of chitosan (CHI) was investigated. Beef samples obtained from a local market were dipped into 1%, 1.5% and 2% chitosan solutions prepared with 1% acetic acid. The samples were drained, vacuum packed and stored at 4°C for a period of 12 days. The samples were evaluated for sensorial properties (color, odor and overall acceptability) and microbial counts (TVC, TCC and TYMC) on 0, 4, 8 and 12 days of storage. Chitosan treated samples having 1%, 1.5%, 2% chitosan solution and control which were expressed as T1, T2, T3 and T0 respectively. The obtained results showed that addition of chitosan solution, significantly (p&lt;0.05) affected on physicochemical (pH, CP, POV, Cooking Loss), microbiological (TVC, TCC, TYMC) and sensory attributes (color, odor, overall acceptance) compared to control samples at refrigerated temperature. The pH and POV of all the treatment groups increase significantly (p&lt;0.05) compared to control group at different days of interval during storage. The CP and cooking loss of different treatment groups decrease significantly (p&lt;0.05) compared to control group at different days of interval during storage. The results also revealed that the samples were dipped in chitosan solution (1%, 1.5%, 2%) significantly (p&lt;0.05) improved the microbiological quality, sensory attributes and reduced lipid oxidation in beef samples compared to the control samples at different days interval. However, abnormal changes were not determined on the samples treated with chitosan, even on the last day of storage. In beef, storage at 4°C for 12 days, chitosan inhibited the growth of spoilage bacteria, reduce lipid oxidation, putrefaction and resulted in better sensory test. The results indicated that the application of chitosan on the beef samples improve the microbiological quality and extends the shelf life usually 5-8 days, which could an alternative to chemical protective additives.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>230-238</em></strong></p> J Alam HM Murshed SME Rahman DH Oh ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 230 238 10.3329/bjas.v46i4.36963 Effect of feeding whole cottonseed as a supplement on digestibility and rumen fermentation characteristics of sheep <p>An experiment was conducted in vivo to investigate the effects on the apparent feed digestibility and rumen fermentation of feeding whole cottonseed (WCS) as a supplement for sheep fed grass hay (GH). Four different diets (T1, T2, T3 and T4) were used as GH alone, GH plus 150, 300 and 500 g WCS d-1 on fresh basis. The main objectives of the in vivo experiment were to examine digestibility and rumen fermentation characteristics (pH, ammonia and VFA concentration) of sheep fed four diets. The results of this experiment indicated that supplementation of WCS at a level of 500 g d-1 (0.37 of the diet) with GH reduced DM, ADF and NDF digestibility. There was no statistically significant effect on total rumen VFA, but the molar proportions of acetate, propionate and butyrate were altered by the addition of highest rate of WCS. These results suggested that WCS might have reduced the number or activity of cellulolytic rumen microorganisms.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>239-243</em></strong></p> Mr Ismartoyo ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 239 243 10.3329/bjas.v46i4.36964 Microbial assessment of beef in selected areas of Mymensingh district in Bangladesh <p>This study was conducted to evaluate microbial load of beef meat during handling and selling in market. Total 12 samples were collected from <em>K.R</em> market, <em>Sheshmore</em> market, <em>Kewatkhali bazaar</em> and Mymensingh <em>Sadar</em> market. These samples were subjected to determine Total Viable Count (TVC), Total Coliform Count (TCC) and Total Yeast and Mould Count (TYMC) by using standard protocol at 0 hr, 2hrs and 5hrs time intarvel. The microbial counts of beef at different markets were high but there were no significant differences (p&gt;0.05) among the markets. In every cases with the increased of time interval all types of microbial count increased significantly (p&lt;o.o1) than the initial time. Microbial contamination of beef occurs as plant workers and machinery repeatedly touch contaminated surfaces and the carcass. Thoughtful design of operating procedures, especially for flaying and evisceration, can greatly reduce this problem. Frequent washing and sanitizing of hands, tools, and machinery is required in order to remove bacteria before they reach the exposed surface of the carcass.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>244-248</em></strong></p> S Afrin MM Hossain M Khan MD Hossain ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 244 248 10.3329/bjas.v46i4.36965 Biomass and nutritional evaluation of German grass (Echinochloa polystachya) cultivated on floating bed <p>German and Napier grasses were cultivated on floating agricultural bed at the valley of Sylhet Agricultural University (SAU) to find out a suitable fodder for floating bed fodder cultivation. Later, German grass was cultivated on five floating beds at Hemupara of Jaintapur, Sylhet and also produced at land of SAU campus. German grass samples from both floating beds and land, as well as local Bermuda grass from Hemupara were evaluated for assessment of biomass production, proximate and fiber composition. There was no significant difference in length and production of German grass cultivated on floating bed and land cultivation. German grass production cost on floating bed was 2.3 Tk/kg. German grass from floating bed contained more ether extract, Ash, Neutral detergent fiber and cellulose than that from land cultivation. DM, Ash, CP, EE, CF and NFE of German grass on floating beds at Hemupara were 199.7g/kg, 20.1g/kg, 57.2g/kg, 37.2g/kg, 411.8gm/kg and 273.9g/kg respectively. The fiber components of German grass on floating beds were NDF (696.4g/kg), ADF (452.2g/kg), ADL (100.1g/kg), cellulose (244.1g/kg) and hemicellulose (352.1g/kg). German grass can be considered as a suitable fodder for floating bed fodder cultivation when there is long term water logging as an alternative fodder production practice in Bangladesh.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>249-257</em></strong></p> MM Islam MMH Khan MJ Uddin MJ Islam ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 249 257 10.3329/bjas.v46i4.36966 Chaperone-like activities of the two pre-incubated small heat shock proteins of Bombyx mori <p>The important small heat shock proteins (sHSPs) of <em>Bombyx mori </em>sHSP19.9, sHSP20.1, sHSP20.4, sHSP20.8, sHSP21.4 and sHSP23.7 are known to display distinct chaperone like activity (CLA) against a range of non-native protein aggregation during environmental stress. The small heat shock proteins sHSP19.9 and sHSP20.8 are identical polypeptides containing a single Cys residue: Cys-43 and Cys-123 respectively. The current information proposes that sHSPs function to prevent irreversible aggregation sometimes pre-incubation is pre-requisite to enhance their chaperone activities. In an attempt to determine their function, we have examined whether these two proteins have CLA using pre-incubation chaperone assay. The assay was conducted against the aggregation of a non-native protein, bovine liver catalase (BLC), which is readily aggregated at 60° C. Heat induced aggregation of BLC was decreased from 100 to 17% in the presence of pre-incubated sHSP20.8, which was 60% for without pre-incubation at a 1:0.5 molar ratio of BLC to sHSP. Whereas the aggregation was decreased from 100 to 33% in the presence of sHSP19.9 with dithiothreitol (DTT) , which was 67% for without pre-incubation at a 1:0.25 molar ratio of BLC to sHSP. The functional reason for such variation might be due to the position of Cys residue in the amino acid sequence.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>258-265</em></strong></p> MT Hossain Y Aso ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 258 265 10.3329/bjas.v46i4.36967 Effects of Lupinus angustifolius (cv. Arabella and Kalya) on the growth and serum profiles in broilers <p>This work examined the effects of incorporation of exogenous enzyme preparation on growth and performance traits in broilers fed diet containing 400 g/Kg of <em>L. angustifolius</em>(cv. Arabella) of Europe and <em>L. angustifolius (</em>cv. Kalya) obtained from Australia. A maize- soy (MS) based diet served as a control diet and acted as positive, while non-protein control diet acted as a negative control (NPC) and fed ad <em>libitum</em> for 18 days. Growth rate, apparent digestibility of N, fat and excretion of essential minerals were assessed. In comparison to broilers fed maize-soy (MS) diet, broilers fed the <em>L. angustifolius </em> of European origin (EL) diet, consumed less feed, had considerably lower body weight gain, as well as lower apparent digestibility. Addition of Rena phytase to the EL (ELPH) and <em>L. angustifolius of Australian origin</em> (ALPH) increased feed intake, however, the feed gain ratio was found to be decreased. . This study indicates that a diet containing high level of lupin meal is detrimental to feed intake broilers and thus affected their performance. However, when lupin meal diet was supplemented with Rena phytase, performance parameters were not different from those obtained from raw meal. The inclusion of lupin meal with or without exogenous enzyme had significant effect on the mineral content of the faeces and on mineral balance. The results suggest that lupin protrein is involved in the elevated loss of minerals associated with long-term effects of this seed as protein source in poultry. Compared to those of control, the N excretion was in the range of 14.9% (EL), 15.6% (ELPH) and 13.9% (ALPH). On the other hand, digestibility was found to be in the range of 78% (EL) and 73% (ELPH) than the corresponding control fed on (MS) diet. The fat excretion was found to be highest in (ALPH) fed diet 19.1% as compared to those of control. True fat digestibility was found to be at the lowest level n ALPH group. The present work has revealed a very remarkable property of <em>L. angustifolius </em>of Australian lupin, that has caused high level of fat excretion in the droppings of broilers fed this as compared to those of its European counterpart. It is highly likely that Australian lupin due to its gel forming character may have caused this kind of response in fecal lipid excretion.</p><p><strong><em>Bang. J. Anim. Sci. 2017. 46 (4): </em></strong><strong><em>266-272</em></strong></p> M Hassan S Datta S Rahman O Islam M Hossain I Hossain MH Rahman ##submission.copyrightStatement## 2018-06-11 2018-06-11 46 4 266 272 10.3329/bjas.v46i4.36968