Bangladesh Journal of Agricultural Research The official journal of the Bangladesh Agricultural Research Unit (BARI). <br />Full text articles available. Bangladesh Agricultural Research Institute (BARI) en-US Bangladesh Journal of Agricultural Research 0258-7122 Molecular assessment of maize inbred lines (Zea mays L.) Using microsatellite markers <p>Genetic diversity analysis and germplasm characterization are essential steps in plant breeding and molecular markers are proved tool to accomplish. The present study was undertaken at the Molecular Breeding Lab of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI) to determine the genetic relatedness and molecular characterization of 15 maize inbred lines of BARI. In present study, genetic diversity analysis was performed by using 10 SSR primers to evaluate the polymorphisms, among them six primers showed distinct polymorphism between the maize inbred lines. The maize genotypes E81, E144, E08, E167, E102, E142 and E121 were found more diverged (0.9003) compared to other inbred lines. On the other hand, the lowest genetic distance values (0.1501) were found between the genotype E140 and genotype E80 followed by genotype E126 and genotype E140; genotype E140 and genotype E65; genotype E65 and genotype E80 values were identical (0.4502). The genotypes viz. E81, E144, E08, E167, E102, E142 and E121 were found far away from centroid of the cluster and rest of the genotypes were placed around the centroid. The Principal Coordinate Analysis (PCO) helped to visualize four major clusters and showed that seven maize inbred lines (E81, E58, E08, E167, E102, E142 and E121) were far away from the other genotypes. In conclusion, SSR markers enabled discrimination among accessions and provided valuable information for future use in improvement of these genomic resources.</p><p>Bangladesh J. Agril. Res. 43(4): 533-542, December 2018</p> - Maniruzzaman MG Azam S Islam MG Hossain MM Rohman ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 533 542 10.3329/bjar.v43i4.39151 Response of chickpea varieties to boron application in calcareous and terrace soils of Bangladesh <p>An experiment was conducted at Madaripur and Gazipur during <em>rabi </em>(winter) season of 2012-13 and 2013-14 to determine the optimum dose of B for different varieties of chickpea (<em>Cicer arietinum </em>L.). There were 12 treatment combinations comprising three varieties (BARI Chola-5, BARI Chola-8 and BARI Chola-9) and four levels of boron (0, 1, 1.5 and 2 kg ha<sup>-1</sup>) along with a blanket dose of N<sub>20</sub>P<sub>20</sub>K<sub>25</sub>S<sub>10</sub>Zn<sub>2</sub> kg ha<sup>-1</sup>. Boron was applied as H<sub>3</sub>BO<sub>3</sub>. Results showed BARI Chola-9 with 1.5 kg B ha<sup>-1</sup> produced the highest seed yield of 1338 kg ha<sup>- 1</sup> at Madaripur and 2218 kg ha<sup>-1</sup> at Gazipur. Nodulation, nitrogen (N) and protein contents were also found highest for the same variety and B treatment. The other two varieties (BARI Chola-5 and BARI Chola-8) also performed higher yield in the plot receiving 1.5 kg B ha<sup>-1</sup> compared to 1 kg B ha<sup>-1</sup> or 2 kg B ha<sup>-1</sup> at both locations. The results suggest that BARI Chola-9 and 1.5 kg B ha<sup>-1</sup> along with N<sub>20</sub>P<sub>20</sub>K<sub>25</sub>S<sub>10</sub>Zn<sub>2</sub> kg ha<sup>-1</sup> could be used for achieving higher yield of chickpea in calcareous and terrace soils of Bangladesh.</p><p>Bangladesh J. Agril. Res. 43(4): 543-556, December 2018</p> MA Quddus MA Hossain HM Naser N Naher F Khatun ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 543 556 10.3329/bjar.v43i4.39152 Response of broccoli to USG and prilled urea in shallow red-brown terrace soil under Madhupur tract <p>A field experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during the period from 2012-13 to verify the effectiveness of urea super granule (USG) and prilled urea (PU) on the yield and quality of broccoli, to assess the comparative performance of USG and PU on nutrient uptake and nitrogen use efficiency and to evaluate the effect of USG and PU on post-harvest nutrient status in Shallow Red-Brown Terrace Soil of Madhupur Tract (AEZ-28). The experiment was designed in a randomized complete block with three replications having 5 treatments as T<sub>1</sub>: Control, T<sub>2</sub>: USG-N<sub>140</sub>, T<sub>3</sub>: USG-N<sub>160</sub>, T<sub>4</sub>: USG-N<sub>180</sub> and T<sub>5</sub>: PU-N<sub>180</sub>. Result showed that USG performed better than PU. The comparative performance of USG in relation to yield, head quality (ascorbic acid, β-carotene and chlorophyll content), SPAD value, nutrient (NPKS) uptake and N use efficiency was found higher as compared to PU. USG treated broccoli plants gave significantly higher yield where the highest yield (13.49 ton ha-1) was recorded with USG-N<sub>160</sub> kg ha<sup>-1</sup>. Moreover, USG showed higher β-carotene and chlorophyll content over PU and those were increased with increasing levels of N. However, ascorbic acid content was slightly decreased with increasing rate of N fertilizer. Nitrogen, phosphorus and potassium uptake increased with increasing N rate upto USGN<sub>180</sub> but sulphur uptake was increased upto USG-N<sub>160</sub>. Nitrogen use efficiency was higher in USG treated plots than that of PU having the highest value of 111.71% with USG-N<sub>160</sub> kg ha<sup>-1</sup>. Post-harvest soil nutrient status was not significantly influenced by the treatments although it was slightly higher in USG as compared to PU. Considering all, USG @ 160 kg N ha<sup>-1</sup> (USG-N<sub>160</sub>) with other recommended fertilizers (@ 53 kg P, 83 kg K, 20 kg S, 2.0 kg Zn, 1 kg B and 0.8 kg Mo ha<sup>-1</sup>) could be the best USG based fertilizer dose for quality broccoli production in Shallow Red-Brown Terrace Soil of Madhupur Tract.</p><p>Bangladesh J. Agril. Res. 43(4): 557-574, December 2018</p> MJ Hussain AJMS Karim ARM Solaiman MS Islam M Rahman ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 557 574 10.3329/bjar.v43i4.39153 Adoption and profitability of summer tomato cultivation in Jashore district of Bangladesh <p>The study assessed the level of adoption and profitability of summer tomato varieties at farm level. Data were collected from 90 randomly selected tomato farmers of Bagherpara, Jashore Sadar and Jhikorgacha upazila of Jashore district. The results indicated that BARI Hybrid Tomato-4 was highly adopted summer tomato variety (75%) followed by BARI Hybrid Tomato-8 (16%) and ACI summer king tomato variety (9%). The adoption level of ploughing, manure and fertilizer use were low, whereas planting time and irrigation were high. Total cost of production of summer tomato was Tk 584822 per hectare whereas Tk 507355 per hectare was variable cost and fixed cost was Tk 77467 per hectare. Among the cost items mancha preparation cost was the highest (26.89 %) and 26.10 % cost was for labor. The average yield of summer tomato was 50.41 t/ha and gross return was 1542300 tk/ha. On the average, benefit cost ratio was found to be 2.64 on full cost basis and 3.04 on cash cost basis. MoP, zipsum and manure were significant effect on summer tomato cultivation. Attack by pest and disease, lack of seed at proper time, lack of agricultural credit and high cost of production were the major constraints for the adoption of summer tomato.</p><p>Bangladesh J. Agril. Res. 43(4): 575-585, December 2018</p> P Hajong B Sikder S Mondal MA Islam ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 575 585 10.3329/bjar.v43i4.39154 Productivity and profitability of improved versus existing cropping pattern in Kushtia region <p>The trial was conducted at Multi Location Testing (MLT) site under On-Farm Research Division, BARI, Kushtia during the last week of February, 2015 to second week of February, 2017 at farmers’ field condition to cover two cropping cycle of four crops. The main objectives of the trial were to verify the feasibility of growing improved cropping pattern Mustard-Mungbean-T.Aus-T.Aman rice and to compare its productivity and profitability with existing cropping pattern Lentil-Sesame-T.Aman rice. The varieties BARI Sarisha-15, BARI Mung-6, BRRI dhan48 and Binadhan-7 were used for the crop Mustard, Mungbean, T.Aus and T.Aman rice, respectively in the improved cropping pattern, while in case of existing pattern, the varieties were BARI Masur-6, BARI Till-3 and Binadhan-7 for Lentil, Sesame and T.Aman rice, respectively. Findings revealed that the mean crop duration of 340 days were required for one cycle in a year in improved cropping pattern which implied that four crop based cropping pattern was agronomically feasible to replace existing cropping pattern. Total seed/grain yield in terms of REY of improved cropping pattern was 14.85 t ha<sup>-1</sup> year<sup>-1</sup> which was 44% higher than that of existing pattern (10.30 t ha-1 year-1). Mean production efficiency (35.78 kg ha<sup>-1</sup> day<sup>-1</sup>), land use efficiency (93.15%) and labour employment (589 mandays ha<sup>-1</sup> year<sup>-1</sup>) of improved cropping pattern was 51%, 16% and 62%, higher, respectively than that of existing cropping pattern. The mean net economic advangaes of improved cropping pattern was Tk 12677 ha<sup>-1</sup> year<sup>-1</sup> which implied that the improved cropping pattern was economically viable. Moreover, the improved cropping pattern increased cropping intensity, farmers knowledge, skill, and income as well as employment. It also maintained soil health by incorporating mungbean stover and T.Aus rice straw in the soil. Therefore, farmers in Kushtia region of Bangladesh could follow the improved cropping pattern in their high and medium high land for higher productivity and profitability as well as employment generation over existing cropping pattern.</p><p>Bangladesh J. Agril. Res. 43(4): 587-598, December 2018</p> MS Rahman MT Islam MZH Prodhan MK Hasan ASMMR Khan ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 587 598 10.3329/bjar.v43i4.39157 Combining ability and heterosis studyin maize inbreds throughout diallel mating design <p>Combining ability effects were estimated for grain yield and some other important agronomic traits of maize in a 7×7 diallel analysis excluding reciprocals. The variances for general combining ability (GCA) were found significant for yield, days to pollen shedding, days to silking and ear height while it was found non-significant for plant height and number of kernels/ear. Non-significant general combining ability (GCA) variance for plant height and number of kernels/ear indicates that these two traits were predominantly controlled by non- additive type of gene action. Specific combining ability (SCA) was significant for all the characters except yield and days to silking. Non-significant specific combining ability (SCA) variance for yield and days to silking suggests that these two traits were predominantly controlled by additive type of gene action. Both GCA and SCA variances were found significant only in days to pollen shedding and ear height indicated the presence of additive as well as non additive gene effects for controlling the traits. However, relative magnitude of these variances indicated that additive gene effects were more prominent for all the characters studied except days to silking. Parent BIL95 was the best general combiner for both high yield and number of kernels/ear and parent BML4 for dwarf plant type. Two crosses (BML4× BML36 and BIL114× BIL31) exhibited significant and positive SCA effects for grain yield involved low × average and average × average general combining parents. The range of heterosis expressed by different crosses for grain yield and days to silking was from -65.83 to 21.26 percent and -17.85 to 8.22 percent, respectively.. The better performing three crosses (BIL114×BIL31, BIL138×BIL95 and BIL31×BIL95) can be utilized for developing high yielding hybrid varieties as well as for exploiting hybrid vigour.</p><p>Bangladesh J. Agril. Res. 43(4): 599-609, December 2018</p> ANMS Karim S Ahmed AH Akhi MZA Talukder A Karim ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 599 609 10.3329/bjar.v43i4.39158 Infestation level and population dynamics of aphid on mustard <p>Mustard <em>(Brassica juncea) </em>variety BARI Shorisha-11 was cultivated during September 2016 to March 2017 in Gazipur, Bangladesh to find out the population dynamics of aphid and its infestation level on mustard. The aphid <em>Lipaphis erysimi </em>(Homoptera: Aphididae) was abundant in the field and caused infestation. The population of aphid in the mustard field showed fluctuation and reached to the peak at 22 December which was 289 per plant. Inflorescence had significantly higher level of infestation compared to individual siliqua and leaf. The daily mean temperature had significant negative, light intensity had insignificant negative and relative humidity had insignificant positive correlation with the abundance of aphid. The multiple linear regression analysis showed that the weather parameters namely temperature, light intensity and relative humidity individually as well as combindly contributed on the abundance of aphid, but the temperature had greater effect than other factors.</p><p>Bangladesh J. Agril. Res. 43(4): 611-618, December 2018</p> E Mandal MR Amin H Rahman AM Akanda ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 611 618 10.3329/bjar.v43i4.39160 Integrated management of tobacco caterpillar and cabbage butterfly with host plant resistant and organic amendment <p>Field study was conducted at Patuakhali Science and Technology University, Patuakhali during 2013-2014 to know the effect of cabbage variety and organic agriculture on the damage potential of <em>Spodoptera litura </em>F. and <em>Pieris brassicae </em>L. Two cabbage varieties viz., Atlas-70 (V<sub>1</sub>) and Super tropic (V<sub>2</sub>), and organic agriculture like, M<sub>0 </sub>= control, M<sub>1</sub> = cowdung @ 6 kgplot<sup>-1</sup>, M<sub>2</sub> = mustard oilcake @ 750gplot<sup>-1</sup>, M<sub>3</sub> = Vermicompost @ 3 kgplot<sup>-1</sup> and M<sub>4</sub> = Trichoderma @ 3 kgplot<sup>-1</sup> were included in this study. Treatment combinations were V<sub>1</sub>M<sub>0</sub>, V<sub>1</sub>M<sub>1</sub>, V<sub>1</sub>M<sub>2</sub>, V<sub>1</sub>M<sub>3</sub>, V<sub>1</sub>M<sub>4</sub>, V<sub>2</sub>M<sub>0</sub>, V<sub>2</sub>M<sub>1</sub>, V<sub>2</sub>M<sub>2</sub>, V<sub>2</sub>M<sub>3</sub> and V<sub>2</sub>M<sub>4</sub>. Results revealed that the lowest number of infested plants/plot and percent infested leavesplant<sup>-1</sup> were found in variety Super tropic and trichoderma (V<sub>2</sub>M<sub>4</sub>) applied plots followed by variety Atlas-70 and vermicompost (V<sub>1</sub>M<sub>3</sub>) applied plots on different dates of observation while the highest number of infested plantsplot-1 and percent infested leaves plant<sup>-1</sup> were in variety Super tropic and mustard oil cake (V<sub>2</sub>M<sub>2</sub>) applied plots followed by variety Atlas-70 and control (V<sub>1</sub>M<sub>0</sub>) applied plots. The highest number and percent (4.00 plot<sup>-1</sup>) and (20.00% plot<sup>-1</sup>) of infested heads were found in variety Super tropic and mustard oil cake (V<sub>2</sub>M<sub>2</sub>) applied plots followed by variety Atlas-70 and control plots (V<sub>1</sub>M<sub>0</sub>) (16.65%). The highest number of healthy heads plot-1 (19.67), healthy head yield (71.47 t ha<sup>-1</sup>) and total head yield (72.14 t ha<sup>-1</sup>) were recorded in variety Super tropic and Tricoderma (V<sub>2</sub>M<sub>4</sub>) applied plots followed by variety Atlas-70 and vermicompost (V<sub>1</sub>M<sub>3</sub>) (70.53 t ha<sup>-1</sup>) applied plots while the lowest number of healthy heads per plot, healthy head yield and total head yield were recorded in in variety Super tropic and mustard oil cake (V<sub>2</sub>M<sub>2</sub>) applied plots followed by variety Atlas-70 and control plots (V<sub>1</sub>M<sub>0</sub>).</p><p>Bangladesh J. Agril. Res. 43(4): 619-630, December 2018</p> S Talukder MMH Khan J Ferdous MO Faruq ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 619 630 10.3329/bjar.v43i4.39161 Design and development of a power groundnut sheller <p>In Bangladesh groundnut shelling is done manually which is laborious, time consuming and costly. Shelling of groundnut pod with the help of mechanical power can be a probable solution of this problem. A power groundnut sheller was designed and fabricated in Farm Machinery and Postharvest Process Engineering (FMPE) Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during 2011-13. The sheller was made of Mild Steel (MS) angle bar, MS flat bar, MS rod, MS sheet, MS sieve, rubber pad etc. The shelling capacities of power groundnut sheller were 110 and 115 kg/h for Dhaka-1 and BARI Badam-8, respectively. Average breakage of groundnut kernel was 2% at 7.5% moisture content (wb). The maximum and minimum unshelled pods were about12.4% and 9.18% for Dhaka -1 and BARI Badam-8, respectively. The shelling efficiency of the power groundnut sheller for Dhaka-1 and BARI Badam-8 were 86.6 and 88.82% respectively at 11.5% moisture content (wb). Winnowing efficiency was found to be 99% in the power groundnut sheller. The use of power groundnut sheller can reduce the cost of shelling by 76% over the manual groundnut sheller. This power groundnut sheller is recommended for shelling of groundnut at farm level and small industry level in Bangladesh.</p><p>Bangladesh J. Agril. Res. 43(4): 631-645, December 2018</p> MA Hoque MZ Hossain MA Hossain ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 631 645 10.3329/bjar.v43i4.39162 Functional and group abundance of insects on eggplant <p>The eggplants (<em>Solanum melongena </em>L.) were cultivated in the field to investigate the abundance and diversity of insects. In total 488 insects were collected from the eggplant field during May to August 2016, which belonged to 20 species in 21 families and 10 orders. Among the taxonomic orders, Hemiptera was the most dominant followed by Coleoptera, Hymenoptera, and Diptera. The order Thysanoptera revealed the lowest abundance. The abundance, richness and diversity of pest, predator, pollinator and other categories of insects differed significantly and the pest revealed the highest abundance and richness compared to others. In total 9 species of insects belonged to 7 families of 4 orders were found as pest and their abundance varied from 0.1±0.1 to 4.6±0.9 /30 sweeps. In total 8 species of insects belonged to 8 families of 7 orders were found as predator and their abundance varied from 0.3±0.1 to 2.0±0.3/30 sweeps. Among the predator insects, lady bird beetle showed the highest abundance. In eggplant field, insects were highest and lowest abundant at 11.00 and 13.00 h of the day, respectively.</p><p>Bangladesh J. Agril. Res. 43(4): 647-653, December 2018</p> MR Amin MS Miah H Rahman NP Nancy MKA Bhuiyan ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 647 653 10.3329/bjar.v43i4.39163 Soil salinity management for increasing potato yield in the coastal area of southern Bangladesh <p>The study was conducted at farmers’ field of Charfashion upazila in Bhola district under AEZ-18 during the <em>Rabi </em>season of 2015-2016 and 2016-2017 to find out suitable soil salinity management practice(s) for maximizing potato yield as well as farmers’ income. Two planting systems viz. raised bed and flat land, and four management systems with fertilizers and mulching were considered in a factorial experiment. The rate of increase in soil salinity in raised beds was significantly lower than that of flat land. On an average, raised bed planting produced 13.04% higher tuber yield than flat land planting. The combination of raised bed + recommended fertilizer (RF) with cowdung @ 5 t ha<sup>-1</sup> as IPNS+ straw mulch (@ 3.5 t ha<sup>-1</sup>) produced the highest tuber yield in both the years (average 21.66 t ha<sup>-1</sup>) and it was 75.10% higher than flat planting system with no mulch. This treatment combination also provided the highest average gross margin (Tk. 115945 ha<sup>-1</sup>) and BCR (2.26). Besides, combination of raised bed + RF+25% K+ straw mulch provided average tuber yield (20.91 t ha<sup>-1</sup>), gross margin (Tk. 110969 ha<sup>-1</sup>) and BCR (2.25), which were very close to above treatment combination. The lowest average potato yield (12.51 t ha<sup>-1</sup>), gross margin (Tk. 39835 ha<sup>-1</sup>) and BCR (1.50) were obtained from combination of flat land planting + RF +no mulching. So, combination of raised bed + mulching+ RF along with IPNS basis cowdung @ 5 tha<sup>-1</sup> or 25% extra K can help minimize soil salinity and produce significantly higher potato yield in the coastal areas.</p><p>Bangladesh J. Agril. Res. 43(4): 655-668, December 2018</p> MS Islam H Saif MR Islam Q Naher ASMMR Khan ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 655 668 10.3329/bjar.v43i4.39164 Assessment of training needs on crop production for farmers in some selected areas of Bangladesh <p>The study attempts to determine the training needs of the farmers emphasizing nine selected major thematic areas. Under each major component, specific and relevant training needs item were collected and systematically incorporated into an interview schedule and administered in terms of frequency of training imparted. Four districts were purposively selected for the study and a total of eighty farmers were randomly selected from four districts. Primary data were analyzed using descriptive statistics. The study revealed that more male was involved in farming and 45% farmers were middle age category (30-39 years). Majority of the farmers completed primary level of education compared to other categories and family size of more than half (60 %) of the respondents was three. Majority number of respondents (57%) had more than 10 years farming experience. A small number of farmers (8.75%) had owned agricultural land and 45% had land between 0.50- 1 hectare. More than 75% of annual gross income of 57.50% farmers came from agricultural activities. More than half (55%) of the respondents collected information on crop and its varieties by own attempt while about 34% was informed from seed seller or dealer. Farmers in Chattogram district had first priority to get training on integrated pest and diseases management, production of bio control agents and bio pesticides, marketing and transportation. Water management, integrated pest and disease management, vermi-compost production, marketing and transportation ranked first in Khagrachori district. The areas of priority for training in Rajshahi district were production and management technology, processing and value addition, marketing and transportation, integrated pest and disease management, water management and vermi-compost production. Training on integrated pest and disease management, bio-control of pests and diseases, production of bio control agents and bio pesticides, production of off-season vegetables, vermi-compost production, marketing and transportation were most emphasized by the respondents in Rangpur district. Respondents defined identification of adulterated fertilizer, insecticide and pesticide application, disease and insects of mango varieties and fruit bagging system of mango as very good type of training. The study concluded that there is an urgent need to design regular training programs in identified thematic areas to fulfill the knowledge gap among the farmers of Bangladesh.</p><p>Bangladesh J. Agril. Res. 43(4): 669-690, December 2018</p> MS Rahman M Khatun ML Rahman SR Haque ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 669 690 10.3329/bjar.v43i4.39165 Intercropping gardenpea (Pisium sativum) with Maize (Zea mays) at farmers’ field <p>An experiment was conducted at the farmers’ field of Phulpur MLT site of On- Farm Research Division, Bangladesh Agricultural Research Institute (BARI), Mymensingh during 2015-16 and 2016-17 to find out a suitable intercrop combination of garden pea with maize for higher productivity and profitability. Five treatments, viz. T<sub>1</sub>= Maize (100%) + one row garden pea (33%) in between maize lines, T<sub>2</sub>= Maize (100%) + two row garden pea (66%) in between maize lines, T<sub>3</sub>= Maize (100%) + garden pea broadcast (100%) in between maize lines, T<sub>4</sub>= Sole maize and T<sub>5</sub>= Sole garden pea were tested following RCB design with six dispersed replications. Maize var. BARI Hybrid Maize-9 and garden pea var. BARI Motorshuti-3 were used in monoculture as well as in intercropping situations. Intercropping of garden pea improved the yield components of maize and offered some additional yield. The highest maize grain yield (8.62 t ha<sup>-1</sup>) and maize equivalent yield (20.22 t ha<sup>-1</sup> yr<sup>-1</sup>) were recorded with maize (100 %) + two rows of garden pea (66 %) in between maize lines (T<sub>2</sub>). The values of all the competition functions were greater than unity and maize (100 %)+ two rows of garden pea (66 %) in between maize lines (T<sub>2</sub>) showed higher values of land equivalent ratio (1.56), gross return (Tk. 311920 ha<sup>-1</sup>), gross margin (Tk. 175697 ha<sup>-1</sup>) and BCR (2.29) as compared to other treatments.</p><p>Bangladesh J. Agril. Res. 43(4): 691-702, December 2018</p> MAH Khan N Sultana N Akter MS Zaman MR Islam ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 691 702 10.3329/bjar.v43i4.39166 In vitro shoot regeneration of mint (Mentha sp. L.) Using different types of explants and levels of benzylaminopurine <p>An experiment was conducted in the Tissue Culture Laboratory of the Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706 from March 2013 to February 2014 for <em>in vitro </em>shoot regeneration of mint using different <em>explant</em>s and levels of benzylaminopurine (BAP) in full strength MS media. Three different types of <em>explant</em>s <em>viz</em>. nodal segment, shoot tip and leaf were evaluated using three levels of BAP (1.0, 2.0 and 3.0 mg/l) along with control for shoot regeneration. Results revealed that shoot tip and nodal segments performed better than leaf as <em>explant</em>s in almost all the characters studied. Shoot tip and nodal segments initiated shoot within the shortest time of 9.6 and 10.6 days, respectively with 1.0 mg/l of BAP. Regarding number of shoot per <em>explant </em>and number of node/shoot, shoot tip and nodal segments along with 1.0 mg/l of BAP performed superior at almost all days after inoculation. In case of interaction of <em>explants </em>and BAP, better performance was recorded in most of the studied parameters from shoot tip and nodal segments alongwith 1.0 mg/l BAP. Therefore, for <em>in vitro </em>shoot regeneration of mint, shoot tip or nodal segment may be used with 1.0 mg/l of BAP.</p><p>Bangladesh J. Agril. Res. 43(4): 703-716, December 2018</p> KT Akter MA Hoque ##submission.copyrightStatement## 2018-12-04 2018-12-04 43 4 703 716 10.3329/bjar.v43i4.39167