Transfection optimisation in HEK-293 cell line

  • Jay Prakash Sah Department of Medical Laboratory Science, School of Health and Allied Sciences, Pokhara University, Lekhnath- 12, Kaski
  • M Salahuddin Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202
  • M Abu Sayed Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200
  • Abu Saim Al Salauddin Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
  • Md Sayfullah College of Medicine, Shaheed Ziaur Rahman Medical College Hospital, Bogra
  • Mahjabin Rashid College of Medicine, Mymensingh Medical College and Hospital, Mymensingh
  • Saidin Saclain Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202
  • Mizanur Rahman Washim Bangladesh Fisheries Research Institute, Mymensingh
  • Muhammad Aurang Zeb Department of Biochemistry, Hazara University Mansehra, Khyber Pakhtunkhwa, Islamic Republic of Pakistan
  • Deluwer Hossain Department of Pharmacology, Bangladesh Agricultural University, Mymensingh-2202
  • Md Rayhan Chowdhury Department of Pharmacy, Stamford University Bangladesh, Dhaka 1217
  • MA Momin Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202
Keywords: cell line, DNA, protein expression, transfection

Abstract

Transfection is the most popular experiments among biological scientist to study the gene expression. But it is really tedious to find out the optimum conditions for protein expression for transfection while working with plasmid DNA. In our present study, the main objective was to setup suitable conditions to study the protein expression. From different conditions, protein expression was observed for 2?g of DNA. We tried different cell number and time frame and got 6.25×105cell/ well of 6 well and 24 hour incubation were the best condition for maximum expression of protein. In our experiment 1:3 ratio of DNA: Lipfectamine was the best for optimum expression of protein in mammalian cell line.

Asian J. Med. Biol. Res. June 2015, 1(1): 121-129

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Author Biography

Jay Prakash Sah, Department of Medical Laboratory Science, School of Health and Allied Sciences, Pokhara University, Lekhnath- 12, Kaski


Published
2015-11-04
How to Cite
Sah, J., Salahuddin, M., Sayed, M., Salauddin, A. S., Sayfullah, M., Rashid, M., Saclain, S., Washim, M., Zeb, M. A., Hossain, D., Chowdhury, M. R., & Momin, M. (2015). Transfection optimisation in HEK-293 cell line. Asian Journal of Medical and Biological Research, 1(1), 121-129. https://doi.org/10.3329/ajmbr.v1i1.25508
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Articles