Asian-Australasian Journal of Bioscience and Biotechnology https://www.banglajol.info/index.php/AAJBB <p><a href="https://www.ebupress.com/journal/aajbb/">Asian-Australasian Journal of Bioscience and Biotechnology</a> is an open access, peer-reviewed, international journal. This journal publishes high-quality original scientific papers and short communications. Review articles of current interest and high standard may be considered.</p> <p style="text-align: justify;">AAJBB is now accepting online submissions through <a href="https://www.banglajol.info/index.php/AAJBB/about/submissions">BanglaJOL’s online journal management system</a>. Authors should register by clicking on the “Register” link at the top of the page. If you have already registered, then just log in using your username and password. To submit a paper, click on the “New Submission” button to start the online procedure.</p> <p><strong>Indexing &amp; Abstracting: </strong>BanglaJOL; Crossref; Google Scholar; JournalSeek</p> Ebu Press Ltd en-US Asian-Australasian Journal of Bioscience and Biotechnology 2414-1283 Occurrence of Clostridium perfringens in layer flocks of selected districts in Bangladesh: molecular typing, antimicrobial susceptibility https://www.banglajol.info/index.php/AAJBB/article/view/60995 <p>Necrotic enteritis (NE) is one of the important enteric diseases in poultry caused by <em>Clostridium perfringens </em>which leads to considerable economic losses for poultry raisers. The present research was carried out to estimate prevalence, risk factors and to confirm molecular pattern, antimicrobial susceptibility status of <em>Clostridium perfringens </em>in layer flocks in Mymensingh and Gazipur districts of Bangladesh, where a total of 287 samples were collected that comprised of water (30), workers’ hand washing (30), and feed (30) as environmental pooled samples; cloacal swab (150) and post mortem sample (intestine) (47) as individual samples, and evaluated by basic (culture, biochemical test), and finally toxinotyping multiplex PCR assays. The flock-level data on potential risk factors were collected through semi-structured interviews with the farm owners and evaluated via univariable and multivariable logistic regression analyses where a <em>p</em>-value of &lt;0.05 was considered as statistically significant. Overall flock-level prevalence of <em>Clostridium perfringens </em>was confirmed to be 6.3% (95% confidence interval [CI]: 3.8-9.7%). The occurrence of <em>C. perfringens </em>was found to be higher in the intestine samples (12.8%, 95% CI: 4.8- 25.7%) followed by feed (6.7%, 95% CI: 0.8-22.1%), cloacal swabs (6.0%, 95% CI: 2.8-11.1%) and workers’ hand washing (3.3%, 95% CI: 0.1- 12.2%). History of immune-suppressive disease (like New castle disease/egg drop syndrome/infectious bursal disease) [Odds Ratio (OR) = 22.16, 95% CI: 1.40-349.36, P = 0.027] and flock size more than 3000 birds (OR = 11.16, 95% CI: 1.26-98.25, P = 0.029) were found to be associated with C<em>. perfringen</em>s infection status in the layer flocks. In this study, <em>C. perfringens </em>toxinotype A is circulating in layer flocks with 72.2% of isolates were established as multidrug-resistant as they demonstrated resistance between 3 to 6 antimicrobial agents. Evidence-based control measures to be taken namely use of probiotics, prebiotic, organic acids, and plant extracts as natural alternatives including control of immune-suppressive diseases are needed to minimize the NE infection and antimicrobial resistance in low biosecurity layer flocks.</p> <p>Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 36-49</p> Mohammad Arif Nigar Sultana Sk Shaheenur Islam Arunima Oarin Tresha Sayed Abdullah-Al-Mamun Md Ashiquen Nobi Mohammad Ferdousur Rahman Khan SM Lutful Kabir Copyright (c) 2022 Mohammad Arif, Nigar Sultana, Sk Shaheenur Islam, Arunima Oarin Tresha, Sayed Abdullah-Al-Mamun, Md Ashiquen Nobi, Mohammad Ferdousur Rahman Khan, SM Lutful Kabir http://creativecommons.org/licenses/by/4.0 2022-08-16 2022-08-16 7 2 36 49 10.3329/aajbb.v7i2.60995 Surveillance and prevalence of gastrointestinal parasite of domestic animals in different abattoirs in Bangladesh https://www.banglajol.info/index.php/AAJBB/article/view/60674 <p>The study was conducted to examine the gastro-intestinal parasite infestation of animal at Gopalganj district in Bangladesh during July 2020 to June 2021. Total collected sample were 151, among them 32 cattle and goat 119. Parasites were collected directly from viscera then preserve for further analysis. Age, sex and species of the examined animals were recorded as far as practicable. Overall prevalence of nematodes, cestodes and trematodes were 62.25%, 9.27% and 50.33% respectively. The prevalence of parasites was variable with <em>Paramphistomum </em>spp. (58.29%),<em> Fasciola </em>spp 62.91%, <em>Moniezia </em>spp. (5.3%), <em>Haemonchus </em>spp<em>. </em>(41.06%), <em>Trichuris </em>spp. (3.31%), <em>Oesophagostomum </em>spp. (10.59%), <em>Strongyloides </em>spp<em>. </em>(2.64%), <em>Trichostrongylus </em>spp. (5.29%), <em>Bunostomum </em>spp. (5.96%), Toxocara spp. (25.17%). Parasitic in male and female exhibited no significant variations (p&gt;0.05) between them. The infection was significantly higher in young than in adult. No significant (p&gt;0.05) variation was observed in infection rates between cattle and goats.</p> <p>Asian Australas. J. Biosci. Biotechnol. 2022, 7 (2), 50-56</p> Md Murshed Hasan Mustafa Md Rafiul Islam Md Abul Hashem Md Abdul Alim Md Mukhlesur Rahman Copyright (c) 2022 Md Murshed Hasan Mustafa, Md Rafiul Islam, Md Abul Hashem, Md Abdul Alim, Md Mukhlesur Rahman http://creativecommons.org/licenses/by/4.0 2022-08-28 2022-08-28 7 2 50 56 10.3329/aajbb.v7i2.60674 A cross-sectional study: farmer’s perceptions, practices, and knowledge (PPK) on antimicrobial drug (AMD) and antimicrobial drug resistance (AMDR) at rural households in selected areas of Cumilla district, Bangladesh https://www.banglajol.info/index.php/AAJBB/article/view/60977 <p>In response to unwise and inappropriate antimicrobial drug (AMD) use, antimicrobial drug resistance (AMDR) is rising globally. The present research was performed to find out and evaluate the perceptions, practices, and knowledge (PPK) of the farmers regarding AMD use and AMDR propagation in rural households. The study was conducted using a pre-tested questionnaire upon 200 respondents with a duration of 5 months in selected areas of Cumilla district, Bangladesh. The correlation analysis showed a significant (<em>p</em>&lt;.01) relationship between farmers’ knowledge and practicing proper administration of drugs in the animal. The sex of the respondents was also found to be correlated with the understanding of Antibiotic Resistance (AR) (<em>p&lt;.</em>01), AR transmission (<em>p</em>&lt;.05), and propagation from livestock to humans (<em>p</em>&lt;.01). Additionally, through the qualitative analysis, we found the powerful impacts of media availability and accessibility, participation in the training program, and good quality education on the improvement of farmers' PPK. Therefore, the government must need to emphasize this topic and educate the farmers about AR to control the spread of AR in order to secure both animal and public health.</p> <p>Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 57-74</p> Abu Sayed Sabiha Akter Ali Hossain Roni Wahedul Karim Ansari Copyright (c) 2022 Abu Sayed, Sabiha Akter, Ali Hossain Roni, Wahedul Karim Ansari http://creativecommons.org/licenses/by/4.0 2022-08-29 2022-08-29 7 2 57 74 10.3329/aajbb.v7i2.60977 Motion characteristics and plasma integrity evaluation of Murrah buffalo semen https://www.banglajol.info/index.php/AAJBB/article/view/61097 <p>Buffalo semen collected from Murrah bull were cryopreserved and evaluated for different motility parameter, kinematics and plasma membrane integrity. Buffalo bulls were maintained uniform standard management and nutritional practices. Semen was collected regularly twice a week semen collection schedule from four (04) Murrah bull. Collected semen was immediately transported to laboratory and evaluated for different macroscopic parameter (color, volume and thickness). Fresh semen was then diluted with saline solution and evaluated for sperm concentration, motility, sperm kinematics and morphology. Semen samples that fill all the standard were selected for freezing and diluted with Tris-egg yolk citrate diluter. Diluted semen was equilibrated, cryopreserved and finally evaluated for post thaw sperm quality. Different motility parameter (total, progressive, static and slow motility) varied significantly (p&lt;0.01) irrespective of different freezing stages. Significantly higher progressive sperm motility and viability of buffalo spermatozoa were observed at fresh semen whereas lower progressive sperm motility and viability was found at post thaw stage. Total and progressive motility reduced by 2.5 and 2.12% following equilibration, whereas following cryopreservation, total and progressive motility reduced by 35.7 and 28.51% and static motility increases accordingly (35.4%). Significantly higher plasma membrane integrity of sperm was observed at fresh semen followed by pre freeze and post thaw semen. Following freezing, integrity of plasma membrane reduces at the rate of 10.81% and 26.7% at pre freezing and post thaw stages. Significantly higher average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were found for fresh semen followed by pre-freeze and post-thaw semen. Frozen buffalo semen with higher progressive motility and motion characteristics may be produced if motility losses can be reduced during freezing stage as this stage results higher motility losses.</p> <p>Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 75-81</p> Md Faizul Hossain Miraz Gautam Kumar Deb SM Jahangir Hossain Shahrina Akter Copyright (c) 2022 Md Faizul Hossain Miraz, Gautam Kumar Deb, Sheikh Mohammad Jahangir Hossain, Shahrina Akter http://creativecommons.org/licenses/by/4.0 2022-08-29 2022-08-29 7 2 75 81 10.3329/aajbb.v7i2.61097