Evaluation of a poly-herbal preparation for the treatment of peptic ulcer

Zaman Hayat, Mueen Ahmad Chaudhry, . Alamgeer


The aim of this study was to validate the traditional uses of ulcerene, a poly-herbal formulation in ethanol, aspirin and stress-induced gastric ulcer model of rat. The extent of gastric ulcer formation was studied, using ulcer score, ulcer index, percentage cure through gross examination and histopathological evaluation. A significant (p<0.001) dose-dependent anti-ulcerant effect was observed in ulcerene (50 and 100 mg/kg)-treated group with highest effectiveness against ethanol-induced ulcer. The concentration-dependent spasmolytic effect was seen in spontaneously contracting, high K+ (80 mM) and carbachol (1 µM)-induced jejunum contractions (10, 0.3 and 1 mg/mL), similar to dicyclomine (10, 1 and 3 µM), indicated non-specific spasmolytic mechanism behind the effect. By considering these results, ulcerene can be suggested for the treatment of peptic ulcer.


Peptic ulcer; Poly-herbal

Full Text:

Download Read


Al Batran R, Al-Bayaty F, Al-Obaidi MMJ, Abdualkader AM, Hadi HA, Ali HM, Abdulla MA. In vivo anti-oxidant and antiulcer activity of Parkia speciosa ethanolic leaf extract against ethanol-induced gastric ulcer in rats. PLoS One. 2013; 8: e64751.

Alam S. Evaluation of antiulcer and anti-oxidant activity of polyherbal formulation in Wistar rats. Int J Pharm Phytopharm Res. 2013; 2.

Brodie DA, Marshall RW, Moreno OM. Effect of restraint on gastric acidity in the rat. Am J Physiol Legacy Content. 1962; 202: 812-14.

Caso JR, Leza JC, Menchen L. The effects of physical and psychological stress on the gastrointestinal tract: Lessons from animal models. Curr Mol Med. 2008; 8: 299-312.

Chaudhary MA, Imran I, Bashir S, Mehmood MH, Rehman Nu, Gilani AH. Evaluation of gut modulatory and bronchodilator activities of Amaranthus spinosus Linn. BMC Complement Altern Med. 2012; 12: 166.

Chioma A, Obiora A, Chukwuemeka U. Does the African garden egg offer protection against experimentally induced ulcers? Asian Pac J Trop Med. 2011; 4: 163-66.

Coblijn UK, Goucham AB, Lagarde SM, Kuiken SD, van Wagensveld BA. Development of ulcer disease after Roux-en-Y gastric bypass, incidence, risk factors, and patient presentation: A systematic review. Obes Surg. 2014; 24: 299-309.

Desai JK, Goyal RK, Parmar NS. Characterization of dopamine receptor subtypes involved in experimentally induced gastric and duodenal ulcers in rats. J Pharm Pharmacol. 1999; 51: 187-92.

Downie J, Twiddy D, Awad S. Antimuscarinic and noncompetitive antagonist properties of dicyclomine hydrochloride in isolated human and rabbit bladder muscle. J Pharmacol Exp Ther. 1977; 201: 662-68.

Goel RK, Gupta S, Shankar R, Sanyal AK. Anti-ulcerogenic effect of banana powder (Musa sapientum var. paradisiaca) and its effect on mucosal resistance. J Ethnopharmacol. 1986; 18: 33-44.

Griggs J, Towers G, Taylor R. The effects of storage on the biological activity of medicinal plants from Nepal. J Ethnopharmacol. 2001; 77: 247-52.

Hollander D, Tarnawski A, Krause WJ, Gergely H. Protective effect of sucralfate against alcohol-induced gastric mucosal injury in the rat. Gastroenterol 1985; 88: 366-74.

Jia YT, Wei W, Ma B, Xu Y, Liu WJ, Wang Y, Lv KY, Tang HT, Wei D, Xia ZF. Activation of p38 MAPK by reactive oxygen species is essential in a rat model of stress-induced gastric mucosal injury. J Immunol. 2007; 179: 7808-19.

Mahattanadul S, Ridtitid W, Nima S, Phdoongsombut N, Ratanasuwon P, Kasiwong S. Effects of Morinda citrifolia aqueous fruit extract and its biomarker scopoletin on reflux esophagitis and gastric ulcer in rats. J Ethnopharmacol. 2011; 134: 243-50.

Mann A, Banso A, Clifford L. An antifungal property of crude plant extracts from Anogeissus leiocarpus and Terminalia avicennioides. Tanzan J Health Res. 2008; 10: 34-38.

Massignani JJ, Lemos M, Maistro EL, Schaphauser HP, Jorge RF, Sousa JPB, Bastos JK, de Andrade SF. Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on different experimental models in rats. Phytother Res. 2009; 23: 1355-60.

Mushtaq S, Chaudhry MA, Rahman HMA. Calcium channels blocked activity: Providing the basis for medicinal use of Abies pindrow in diarrhea and bronchitis. Bangladesh J Pharmacol. 2015; 10: 430-35.

Naz SB, Chaudhary MA, Ul Rehman MS. Dual receptor blocker mechanism arbitrates smooth muscle relaxant effect of Polypodium vulgare Linn. Bangladesh J Pharmacol. 2016; 11: 414-20.

Panda V, Sonkamble M. Anti-ulcer activity of Ipomoea batatas tubers (sweet potato). Functional Foods Health Dis. 2012; 2: 48-61.

Sener G, Paskaloglu K, Ayanoglu-Dülger G. Protective effect of increasing doses of famotidine, omeprazole, lansoprazole, and melatonin against ethanol-induced gastric damage in rats. Indian J Pharmacol. 2004; 36: 171.

Takeuchi K. Pathogenesis of NSAID-induced gastric damage: Importance of cyclooxygenase inhibition and gastric hypermotility. World J Gastroenterol. 2012; 18: 2147-60.

Ukwuani A, Ihebunna O, Samuel R, Peni I. Acute oral toxicity and antiulcer activity of Piliostigma thonningii leaf fraction in albino rats. Bull Env Pharmacol Life Sci. 2012; 2: 41-45.

ur Rahaman MS, Chaudhary MA, Bashir A, Alamgeer A. Rationalization of traditional uses of Berberis lycium in gastrointestinal disorders. Br J Med Med Res. 2013; 3: 868-79.

Wallace JL, McKnight W, Reuter BK, Vergnolle N. NSAID-induced gastric damage in rats: Requirement for inhibition of both cyclooxygenase 1 and 2. Gastroenterol 2000; 119: 706-14.

Yedgar S, Krimsky M, Cohen Y, Flower RJ. Treatment of inflammatory diseases by selective eicosanoid inhibition: A double-edged sword?. Trends Pharmacol Sci. 2007; 28: 459-64.

DOI: http://dx.doi.org/10.3329/bjp.v12i2.29980

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Copyright (c) 2017 Zaman Hayat, Mueen Ahmad Chaudhry, Alamgeer

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is a single-blinded peer-reviewed, video component, open access official publication of the Bangladesh Pharmacological Society (BDPS). eISSN: 1991-0088. For contact: Editor-In-Chief