Antihyperglycemic and anti-oxidant activity of various fraction of Parmotrema hababianum in streptozotocin-induced diabetic rat

Ayyappadasan Ganesan, Amutha Mahesh, Jeya Prakash Sundararaj, Kalaiselvi Mani, Ponmurugan Ponnusamy


The core objective of this study was to investigate the in vitro anti-oxidant and antihyperglycemic effect of the ethanol extract of Parmotrema hababianum in streptozocin-induced diabetic rats for 42 days. The extract showed nearly all anti-oxidant activities with maximum presence. The treatment with extract in diabetic rats at the dosage level of 100 and 200 mg/kg, which is compared with diabetic control and glibenclamide at a dosage level of 4 mg/kg and the biochemical parameters such as blood glucose, total cholesterol, triglycerides, HDL, LDL, insulin, total protein, urea and creatinine were assessed. The extract showed positive correlation (p<0.001) in reducing blood glucose level as compared to the control. Moreover, there was a significant (p<0.01) decrease in TC, TG, LDL, SGPT, SGOT, urea and creatinine level and significant (p<0.01) increase in HDL and insulin level. Thus, the results of this study show considerable efficacy in curing diabetes with potent anti-oxidant and anti-hyperglycemic activity.

Video Clip of Methodology:

6 min 12 sec  Full Screen   Alternative 


Anti-hyperglycemic; Anti-oxidant; Diabetic; Parmotrema hababianum; Rat; Streptozotocin

Full Text:

Download Read


Atalay F, Halici MB, Mavi A, Cakir A, Odabasoglu F, Kazaz C, Aslan A, Kufrevioglu OI. Anti-oxidant phenolics from Lobaria pulmonaria (L.) Hoffm and Usnea longissima Ach. Lichen species. Turk J Chem. 2011; 35: 64761.

Brown JE, Rice-Evans CA. Luteolin-Rich artichoke extract protects low density lipoprotein from oxidation in vitro. Free Radic Res. 1998; 29: 247-55.

Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, Remberg SF. Systematic screening of total anti-oxidants in dietary plants. J Nutr. 2002; 132: 46171.

Jeya PS, Selvambigai K, Ayyappadasan G, Ponmurugan P, Sanjeeva N. In vitro assessment of anti-oxidant and antimicrobial activities of different solvent extracts from lichen Ramalina nervulosa. Int J pharm and Pharm Sci. 2015; 7: 200-04.

Kesavalu M, Kemeshwara M, Rao B, Giri R. Lipid peroxidation and anti-oxidant enzyme status in type II diabetics with coronary heart disease. Diabetes Res Clin Pract. 2001; 53: 33-39.

Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H. Anti-oxidant activity of some common plants. Turk J Biol. 2008; 32: 51-55.

Ku G, Kegels G. The performance of the Finnish diabetes risk score: A modified Finnish diabetes risk score and a simplified Finnish diabetes risk score in community-based cross-sectional screening of undiagnosed type 2 diabetes in the Philippines. Prim Care Diabetes. 2013; 7: 249-59.

Liaqat H, Muhammad IQ, Shafiq-ur-Rehman. Antihyperglycemic and hypolipidemic potential of caesalpinia decapetala in alloxan-induced diabetic rabbits. Bangladesh J Pharmacol. 2014; 9: 529-32.

Maruthapandian A, Mohan VR. Antidiabetic, antihyperlipidaemic and anti-oxidant activity of Pterocarpus marsupium Roxb in alloxan-induced diabetic rats. Int J Pharm Tech Res. 2011; 3: 1681-87.

Muller K. Pharmaceutically relevant metabolites from lichens. Appl Microbiol and Biotech. 2001; 56: 9-16.

Oboh G, Puntel RL, Rocha JBT. Hot pepper (Capsicum annuum, Tepin and Capsicum Chinese, Habanero) prevents Fe2+ induced lipid peroxidation in brain in vitro. Food Chem. 2007; 102: 178-85.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr. 1986; 44: 307-15.

Ponnusamy R, Thangaraj P. Antidiabetic analysis and insulin expression study of a wild leafy vegetable Acalypha alnifolia Klein ex wild. on streptozotocin-induced diabetic rats. Bangladesh J Pharmacol. 2015; 10: 461-66.

Ruch RJ, Cheng, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by anti-oxidant catechins isolated from Chinese green tea. Carcinogenesis 1989; 10: 1003-08.

Sangameswaran B, Balakrishnan BR, Chumbhale D, Jayakar B. In vitro anti-oxidant activity of roots of Thespesia lampas. Pakistan J Pharm Sci. 2009; 22: 368-72.

Shih PW, Lai PL, William JH. Anti-oxidant activities of aqueous extracts of selected plants. Food Chem. 2006; 99: 775-83.

Slinkard K, Slingleton VL. Total phenolic analyses: Automation and comparison with manual method. Am J Enol Vitic. 1997; 28: 49-55.

Soxhlet F. Die gewichtsanalytische Bestimmung des Milchfettes. Polytech J (Dingler's). 1879; 232: 461-65.

Tung YT, Wu JH, Huang CY, Ku YH, Chang ST. Anti-oxidant activities and phytochemical characteristics of extracts from Acacia confusa bark. Bioresour Technol. 2009; 100: 509-14.

Walker FJ, James PW. A revised guide to microchemical technique for the identification of lichen products. Bull Bri Lic Soc. 1980; 46: 13-29.

Xu Y, He Z, King GL. Introduction of hyperglycemia and dyslipidemia in the pathogenesis of diabetic vascular complications. Curr Diab Rep. 2005; 5: 91-97.


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Copyright (c) 2016 Ayyappadasan Ganesan, Amutha Mahesh, Jeya Prakash Sundararaj, Kalaiselvi Mani, Ponmurugan Ponnusamy

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is a single-blinded peer-reviewed, video component, open access official publication of the Bangladesh Pharmacological Society (BDPS). eISSN: 1991-0088. For contact: Editor-In-Chief